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Serum and plasma can be used for analyte studies, including proteins, lipids, and small molecules, and as a source of cell-free nucleic acids. Cell concentrates are used in functional studies, flow cytometry, culture experiments, or as a source for cellular nucleic acids. Before initiating studies on blood, a thorough evaluation of practices that may influence analyte and\/or cellular integrity is required. Thus, it is imperative that child health researchers working with human blood are aware of how experimental results can be altered by blood sampling methods, times to processing, container tubes, presence or absence of additives, shipping and storage variables, and freeze-thaw cycles. The authors of this review, in an effort to encourage and optimize translational research using blood from pediatric patients, outline best practices for blood collection, processing, shipment, and storage.<\/jats:p>","DOI":"10.1542\/peds.2012-1181","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T01:50:45Z","timestamp":1363657845000},"page":"754-766","source":"Crossref","is-referenced-by-count":69,"title":["Translational Research in Pediatrics II: Blood Collection, Processing, Shipping, and Storage"],"prefix":"10.1542","volume":"131","author":[{"given":"Carolina","family":"Gillio-Meina","sequence":"first","affiliation":[{"name":"Translational Research Centre, London, Ontario, Canada;"},{"name":"Children\u2019s Health Research Institute, London, Ontario, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gediminas","family":"Cepinskas","sequence":"additional","affiliation":[{"name":"Centre for Critical Illness Research,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin L.","family":"Cecchini","sequence":"additional","affiliation":[{"name":"Translational Research Centre, London, Ontario, Canada;"},{"name":"Children\u2019s Health Research Institute, London, Ontario, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas D.","family":"Fraser","sequence":"additional","affiliation":[{"name":"Translational Research Centre, London, Ontario, Canada;"},{"name":"Children\u2019s Health Research Institute, London, Ontario, Canada;"},{"name":"Centre for Critical Illness Research,"},{"name":"Critical Care Medicine and Pediatrics,"},{"name":"Clinical Neurologic Sciences, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"417","published-online":{"date-parts":[[2013,4,1]]},"reference":[{"issue":"1","key":"2021120903224364300_B1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1542\/peds.2011-0134","article-title":"Translational research in pediatrics: tissue sampling and biobanking.","volume":"129","author":"Brisson","year":"2012","journal-title":"Pediatrics"},{"key":"2021120903224364300_B2","unstructured":"International Society for Biological and Environmental Repositories. 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