{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:19:10Z","timestamp":1780075150028,"version":"3.54.0"},"reference-count":14,"publisher":"American Academy of Pediatrics (AAP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>Antidense fine speckles 70 (anti-DFS70) antibodies, a peculiar antinuclear antibody (ANA) pattern by indirect immunofluorescence, is frequently observed in ANA-positive individuals with no evidence of systemic autoimmune rheumatic disease. They may be found in many different inflammatory conditions and in healthy individuals. We herein report a case of an 8-year-old girl presenting with generalized edema, hypertension, hepatomegaly, and a history of pharyngitis, which occurred 3 weeks earlier. Laboratory analysis revealed low complement C3 (6 mg\/dL), microhematuria, and proteinuria. A diagnosis of acute glomerulonephritis was made. Anti-dsDNA, antiextractable nuclear antigens, and antineutrophil cytoplasmic antibodies were negative. However, a highly positive (1:640) ANA immunofluorescence test with dense fine speckles pattern was found. The presence of anti-DFS70 immunoglobulin G antibodies was confirmed by a specific immunoassay. In conclusion, the presence of isolated anti-DFS70 antibodies may be useful to exclude an autoimmune pathogenesis in those children with a positive ANA test and a clinical picture possibly attributable to systemic autoimmune rheumatic disease. This will avoid further unnecessary investigation with the potential for incorrect diagnosis and possibly harmful treatment.<\/jats:p>","DOI":"10.1542\/peds.2013-3914","type":"journal-article","created":{"date-parts":[[2014,11,11]],"date-time":"2014-11-11T01:34:00Z","timestamp":1415669640000},"page":"e1706-e1708","source":"Crossref","is-referenced-by-count":22,"title":["Anti-DFS70 Antibodies: A Useful Biomarker in a Pediatric Case With Suspected Autoimmune Disease"],"prefix":"10.1542","volume":"134","author":[{"given":"Martina","family":"Fabris","sequence":"first","affiliation":[{"name":"Institute of Clinical Pathology,"},{"name":"Institute of Clinical Pathology, DSMB, University of Udine, Udine, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Zago","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pathology, DSMB, University of Udine, Udine, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaello","family":"Tosolini","sequence":"additional","affiliation":[{"name":"Pediatric Clinic, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Melli","sequence":"additional","affiliation":[{"name":"Pediatric Clinic, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Bizzaro","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pathology, San Antonio Hospital, Tolmezzo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elio","family":"Tonutti","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology and Allergy, University Hospital S. Maria Misericordia, Udine, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"417","published-online":{"date-parts":[[2014,12,1]]},"reference":[{"key":"2021120906262965700_B1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1749-6632.2009.05127.x","article-title":"Epitope specificity and significance in systemic autoimmune diseases.","volume":"1183","author":"Mahler","year":"2010","journal-title":"Ann N Y Acad Sci"},{"issue":"1","key":"2021120906262965700_B2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1136\/annrheumdis-2013-203863","article-title":"International recommendations for the assessment of autoantibodies to cellular antigens referred to as anti-nuclear antibodies.","volume":"73","author":"Agmon-Levin","year":"2014","journal-title":"Ann Rheum Dis"},{"issue":"3","key":"2021120906262965700_B3","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0022-5347(17)35023-1","article-title":"Autoantibodies in interstitial cystitis.","volume":"151","author":"Ochs","year":"1994","journal-title":"J Urol"},{"issue":"6 pt 1","key":"2021120906262965700_B4","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1067\/mai.2000.107039","article-title":"Autoantibodies to DFS 70 kd\/transcription coactivator p75 in atopic dermatitis and other conditions.","volume":"105","author":"Ochs","year":"2000","journal-title":"J Allergy Clin Immunol"},{"issue":"9","key":"2021120906262965700_B5","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.autrev.2011.11.005","article-title":"Importance of the dense fine speckled pattern on HEp-2 cells and anti-DFS70 antibodies for the diagnosis of systemic autoimmune diseases.","volume":"11","author":"Mahler","year":"2012","journal-title":"Autoimmun Rev"},{"issue":"11","key":"2021120906262965700_B6","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.3899\/jrheum.120598","article-title":"Anti-DFS70\/LEDGF antibodies are more prevalent in healthy individuals compared to patients with systemic autoimmune rheumatic diseases.","volume":"39","author":"Mahler","year":"2012","journal-title":"J Rheumatol"},{"issue":"1","key":"2021120906262965700_B7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jaut.2005.09.001","article-title":"Anti-p80 coilin autoantibodies react with a conserved epitope and are associated with anti-DFS70\/LEDGF autoantibodies.","volume":"26","author":"Goto","year":"2006","journal-title":"J Autoimmun"},{"key":"2021120906262965700_B8","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1196\/annals.1381.019","article-title":"Antibodies to the lens and cornea in anti-DFS70-positive subjects.","volume":"1107","author":"Bizzaro","year":"2007","journal-title":"Ann N Y Acad Sci"},{"issue":"1","key":"2021120906262965700_B9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/art.30084","article-title":"Pattern on the antinuclear antibody-HEp-2 test is a critical parameter for discriminating antinuclear antibody-positive healthy individuals and patients with autoimmune rheumatic diseases.","volume":"63","author":"Mariz","year":"2011","journal-title":"Arthritis Rheum"},{"key":"2021120906262965700_B10","first-page":"494356","article-title":"The clinical significance of the dense fine speckled immunofluorescence pattern on HEp-2 cells for the diagnosis of systemic autoimmune diseases.","volume-title":"Clin Dev Immunol","author":"Mahler","year":"2012"},{"issue":"2","key":"2021120906262965700_B11","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1089\/jop.2000.16.181","article-title":"Review: Age-related cataract: immunity and lens epithelium-derived growth factor (LEDGF).","volume":"16","author":"Shinohara","year":"2000","journal-title":"J Ocul Pharmacol Ther"},{"issue":"3","key":"2021120906262965700_B12","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1002\/art.20095","article-title":"Autoimmunity to the nuclear autoantigen DFS70 (LEDGF): what exactly are the autoantibodies trying to tell us?","volume":"50","author":"Ganapathy","year":"2004","journal-title":"Arthritis Rheum"},{"key":"2021120906262965700_B13","first-page":"703759","article-title":"Clinical phenotypes of patients with anti-DFS70\/LEDGF antibodies in a routine ANA referral cohort.","volume-title":"Clin Dev Immunol","author":"Miyara","year":"2013"},{"issue":"12","key":"2021120906262965700_B14","doi-asserted-by":"crossref","first-page":"4036","DOI":"10.1002\/art.30621","article-title":"Recognizing the dense fine speckled\/lens epithelium-derived growth factor\/p75 pattern on HEP-2 cells: not an easy task! Comment on the article by Mariz et al.","volume":"63","author":"Bizzaro","year":"2011","journal-title":"Arthritis Rheum"}],"container-title":["Pediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/publications.aap.org\/pediatrics\/article-pdf\/134\/6\/e1706\/1092808\/peds_2013-3914.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/publications.aap.org\/pediatrics\/article-pdf\/134\/6\/e1706\/1092808\/peds_2013-3914.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T00:48:22Z","timestamp":1639097302000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.aap.org\/pediatrics\/article\/134\/6\/e1706\/33176\/Anti-DFS70-Antibodies-A-Useful-Biomarker-in-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":14,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,12,1]]},"published-print":{"date-parts":[[2014,12,1]]}},"URL":"https:\/\/doi.org\/10.1542\/peds.2013-3914","relation":{},"ISSN":["0031-4005","1098-4275"],"issn-type":[{"value":"0031-4005","type":"print"},{"value":"1098-4275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}