{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T02:16:43Z","timestamp":1787969803697,"version":"build-2784847793"},"reference-count":19,"publisher":"American Academy of Pediatrics (AAP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:p>Isolation of Ureaplasma spp. from preterm neonates and the association with development of bronchopulmonary dysplasia has been previously investigated. However, few studies have contrasted the nature of infection in twins. In this article, we report that dizygotic twins (1 girl, 1 boy) born at 24 weeks gestation both yielded culturable Ureaplasma from endotracheal secretions. The samples were part of a serial blind collection cohort of ventilated premature neonates, and analysis of repeat cultures showed stable, separate infections over a period of 17 and 21 days, respectively. Immunoblot and probe-specific quantitative polymerase chain reaction analysis determined that Twin 1 was solely infected with Ureaplasma parvum (specifically, serovar 6 by gene sequencing), whereas Twin 2 was solely infected with Ureaplasma urealyticum (specifically, genotype A- serovars 2, 5, and 8 by gene sequencing). Immunoblot analysis found that the major surface antigen (multiple-banded antigen) altered relative mass for both strains during the course of infection. Quantitative polymerase chain reaction analysis of extracted endotracheal aspirates confirmed no evidence of mixed infection for either twin. Failure of sentinel ventilated preterm infants on the same ward to acquire Ureaplasma infection after the first week of birth suggests no cot-to-cot transfer of Ureaplasma infection occurred. This study demonstrated not only a contrasting clinical outcome for a set of twins infected with 2 separate species of Ureaplasma, but also the first real-time demonstration of multiple-banded antigen alteration and evolution of Ureaplasma over the course of a clinical infection.<\/jats:p>","DOI":"10.1542\/peds.2016-0565","type":"journal-article","created":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T11:09:00Z","timestamp":1468494540000},"source":"Crossref","is-referenced-by-count":6,"title":["Isolation of Separate <i>Ureaplasma<\/i> Species From Endotracheal Secretions of Twin Patients"],"prefix":"10.1542","volume":"138","author":[{"given":"Michael L.","family":"Beeton","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola C.","family":"Maxwell","sequence":"additional","affiliation":[{"name":"Neonatal ICU, Derriford Hospital, Plymouth, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria J.","family":"Chalker","sequence":"additional","affiliation":[{"name":"Respiratory & Vaccine Preventable Bacteria Reference Unit, Public Health England, London, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca J.","family":"Brown","sequence":"additional","affiliation":[{"name":"Respiratory & Vaccine Preventable Bacteria Reference Unit, Public Health England, London, United Kingdom; and"},{"name":"Division of Infection and Immunity, School of Medicine, Cardiff University, University Hospital of Wales, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali F.","family":"Aboklaish","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, School of Medicine, Cardiff University, University Hospital of Wales, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. 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