{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:11:10Z","timestamp":1710288670423},"reference-count":40,"publisher":"Universidad Nacional de Colombia","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. salud p\u00fablica"],"abstract":"<jats:p>&lt;p&gt;Objetivo Comparar secuencias de nucle\u00f3tidos y de amino\u00e1cidos de la prote\u00edna no estructural 1-NS1 de cepas DENV-2, aisladas de pacientes febriles de diferentes paises suramericanos, que cursaron cuadros cl\u00ednicos con severidad o sin ella.Materiales y M\u00e9todos El an\u00e1lisis filogen\u00e9tico fue realizado a partir de 28 secuencias moleculares completas (1\u00a0056 pb) del gen NS1 del serotipo DENV-2. Se realiz\u00f3 un an\u00e1lisis filogen\u00e9tico bayesiano utilizando el software MrBayes v.3.2.0, con el modelo SYM+G y un an\u00e1lisis filogen\u00e9tico con el m\u00e9todo Neighbor-Joining con el modelo Jukes-Cantor. Adem\u00e1s, las secuencias de amino\u00e1cidos fueron alineadas y comparadas entre s\u00ed, mediante el programa Clustal W incluido en el software MEGA v. 5.2.Resultados En las secuencias de amino\u00e1cidos asociadas a sangrado, la sustituci\u00f3n m\u00e1s frecuente fue isoleucina\u00ae treonina, en la posici\u00f3n 93. Estas secuencias presentaron un mayor porcentaje (94,6\u00a0%) de homolog\u00eda de amino\u00e1cidos de la proteina NS1 en comparaci\u00f3n con el porcentaje de homolog\u00eda (74\u00a0%) de los aislamientos DENV-2 no asociados a sangrado. Se identificaron cinco clados que agrupan la mayor\u00eda de las secuencias analizadas (19\/24; 79,2\u00a0%) con valores de probabilidad posterior mayores o iguales al 58\u00a0%. Siete (87,5\u00a0%) secuencias asociadas a sangrado se relacionan filogen\u00e9ticamente dentro de los clados 4 y 5, con valores de probabilidad posterior del 58\u00a0% y 97\u00a0%, respectivamente.Conclusion No se encontraron caracter\u00edsticas filogen\u00e9ticas ni tampoco diferencias entre las secuencias de amino\u00e1cidos de la prote\u00edna NS1-DENV-2 estudiadas, que pudieran ser relacionadas, de manera directa, con la severidad de la enfermedad.&lt;\/p&gt;<\/jats:p>","DOI":"10.15446\/rsap.v18n3.44078","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T09:58:39Z","timestamp":1467194319000},"page":"459","source":"Crossref","is-referenced-by-count":0,"title":["An\u00e1lisis filogen\u00e9tico de secuencias suramericanas del gen ns1 del serotipo dengue-2, relacionadas con sangrado cl\u00ednico severo"],"prefix":"10.15446","volume":"18","author":[{"given":"Raimundo","family":"Castro Orozco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyda Raquel","family":"Castro Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris Esther","family":"G\u00f3mez Camargo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6146","published-online":{"date-parts":[[2016,6,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1110265"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0074"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0346"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis351"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.06.018"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/PEC.0b013e31828ed30e"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1590\/S1726-46342011000100011"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002095"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/1471-2180-10-127","article-title":"Serotype infectivity and phylogenetic of dengue virus cause of dengue fever (DF), dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS) in surabaya-Indonesia.","volume":"1","author":"Rantam","year":"2013","unstructured":"9. 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