none 10.15446/rsap.v19n5.52317 Universidad Nacional de Colombia Universidad Nacional de Colombia 6146 98493575 64710 1526500862 10.15446 2024-03-12T17:19:13Z 2018-05-16T22:27:15Z 3 Revista de Salud Pública Rev. salud pública 2539-3596 0124-0064 09 01 2017 19 5 Elkin Enrique Hernández Porras Liliana Elisa Rosero Torres Eliana Liseth Parra Barrera Jaime Alberto Guerrero Montilla Adriana Leonor Gómez Rubio Jaime Enrique Moreno Castañeda Objetivo Aplicar una técnica de reacción en cadena de la polimerasa (PCR) múltiple en tiempo real para la detección de Salmonella spp., Listeria monocytogenes y Yersinia enterocolitica, como herramienta de apoyo diagnóstico en la vigilancia de brotes de enfermedad transmitida por alimentos.Materiales y Métodos Se aplicó la metodología molecular en muestras clínicas provenientes de individuos que estaban asociados a brotes de enfermedad transmitida por alimentos de dos departamentos de Colombia. Los resultados se compararon con los datos arrojados por la metodología convencional de cultivo. Adicionalmente a los aislamientos obtenidos se les evaluó relación clonal mediante la técnica de electroforesis de campo pulsado (PFGE).Resultados Se determinó un total de 123 casos de enfermedad transmitida por alimentos de los cuales 45 muestras biológicas fueron confirmadas por laboratorio y 88 mediante nexo epidemiológico. La metodología molecular detectó 35/45 muestras positivas frente a 17/45 muestras positivas detectadas mediante la metodología convencional. La PFGE demostró relación clonal en cada brote.Conclusión Los resultados del estudio demuestran la aplicabilidad de la técnica molecular como herramienta útil de apoyo diagnóstico en la caracterización de brotes de enfermedad transmitida por alimentos, permitiendo una respuesta oportuna y confiable. 09 01 2017 671 678 http://creativecommons.org/licenses/by/4.0 10.15446/rsap.v19n5.52317 https://revistas.unal.edu.co/index.php/revsaludpublica/article/view/52317 https://revistas.unal.edu.co/index.php/revsaludpublica/article/viewFile/52317/65947 https://revistas.unal.edu.co/index.php/revsaludpublica/article/viewFile/52317/65947 10.1111/j.1753-4887.2010.00286.x Nyachuba DG. Foodborne illness: is it on the rise? Nutr Rev. 2010; 68(5):257-69. 10.1186/1471-2458-14-509 McLinden T, Sargeant JM, Thomas MK, Papadopoulos A, Fazil A. Component costs of foodborne illness: a scoping review. BMC Public Health. 2014; 14:509. 10.1016/j.tmaid.2011.11.001 Sánchez-Vargas FM, Abu-El-Haija MA, Gómez-Duarte OG. Salmonella infections: an update on epidemiology, management, and prevention. Travel Med Infect Dis. 2011; 9(6):263-77. 10.1093/eurpub/ckt126.200 Maertens de Noordhout C, Devleesschauwer B, Angulo FJ, Verbeke G, Haagsma J, Kirk M, Havelaar A, Speybroeck N. The global burden of listeriosis: a systematic review and meta-analysis. Lancet Infect Dis. 2014; 14 (sup. 1) 70870-9. 10.3201/eid1901.120393 Cartwright EJ, Jackson KA, Johnson SD, Graves LM, Silk BJ, Mahon BE. Listeriosis outbreaks and associated food vehicles, United States, 1998-2008.Emerg Infect Dis. 2013; 19(1):1-9 10.4061/2011/182051 Galindo CL, Rosenzweig JA, Kirtley ML, Chopra AK. Pathogenesis of Y. enterocolitica and Y. pseudotuberculosis in Human Yersiniosis. J Pathog. 2011;182051. 10.1111/j.1600-0463.2011.02767.x Hoorfar J. Rapid detection, characterization, and enumeration of foodborne pathogens. APMIS Suppl. 2011; (133):1-24. Instituto Nacional de Salud. Informe final del evento enfermedades transmitidas por alimentos, año 2013. SIVIGILA. 10.2353/jmoldx.2010.090036 Muñoz N, Diaz-Osorio M, Moreno J, Sanchez-Jimenez M, Cardona-Castro N. Development and Evaluation of a Multiplex Real-Time Polymerase Chain Reaction Procedure to Clinically Type Prevalent Salmonella enterica Serovars. Journal of Molecular Diagnostics. 2010; 12 (2):220-225. 10.1016/j.diagmicrobio.2008.08.012 Long F, Zhu XN, Zhang ZM, Shi XM. Development of a quantitative polymerase chain reaction method using a live bacterium as internal control for the detection of Listeria monocytogenes. Diagn Microbiol Infect Dis. 2008; 62(4):374-81. 10.1016/j.ijfoodmicro.2007.11.006 Rantsiou K, Alessandria V, Urso R, Dolci P, Cocolin L. Detection, quantification and vitality of Listeria monocytogenes in food as determined by quantitative PCR. Int J Food Microbiol 2008; 121:99-105. 10.1016/j.idc.2013.05.001 Donnenberg MS, Narayanan S. How to Diagnose a Foodborne Illness. Infect Dis Clin N Am. 2013; (27):535–554. Realpe ME, Montaño LA. Manual de procedimientos para el diagnóstico bacteriológico de enfermedad diarreica bacteriana aguda, identificación de Salmonella spp., Shigella spp., Vibrio cholerae. Instituto Nacional de Salud MNL-R01.001.5030-002. 2011. Disponible en https://goo.gl/twXmuw. Consultado diciembre de 2014. Poppof, M.Y., 8ª Ed, WHO Centre for Reference and Research on Salmonella, Instituto Pasteur, París, Francia, 2007. Instituto Nacional de Vigilancia de Medicamentos y Alimentos INVIMA. Manual de técnicas de análisis para control de calidad microbiológico de alimentos para consumo humano. Ministerio de Salud, Santa fe de Bogotá D.C.; 1998. 10.1089/fpd.2006.3.59 Ribot EM, Fair MA, Gautom R, Cameron DN, Hunter SB, Swaminathan B, Barrett TJ. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis. 2006; 3(1):59-67. 10.1089/fpd.2010.0787 Hendriksen RS, Vieira AR, Karlsmose S, Lo Fo Wong DM, Jensen AB, Wegener HC, Aarestrup FM, et al Global monitoring of Salmonella serovar distribution from the World Health Organization Global Foodborne Infections Network Country Data Bank: results of quality assured laboratories from 2001 to 2007. Foodborne Pathog Dis. 2011; 8(8):887-900. 10.3201/eid1903.111866 Painter JA, Hoekstra RM, Ayers T, Tauxe RV, Braden CR, Angulo FJ, Griffin PM, et al. Attribution of foodborne illnesses, hospitalizations, and deaths to food commodities by using outbreak data, United States, 1998-2008. Emerg Infect Dis. 2013; 19(3):407-15. 10.1016/j.ijfoodmicro.2011.04.018 Pires SM, Vieira AR, Perez E, Lo Fo Wong D, Hald T. Attributing human foodborne illness to food sources and water in Latin America and the Caribbean using data from outbreak investigations. Int J Food Microbiol. 2012; 152(3):129-38. 10.1017/S095026881300037X Miller T, Braun PG, Fehlhaber K, Prager R, Pfeifer Y, Rabsch W, et al. Typing of Salmonella enterica serovar Infantis isolates from 51 outbreaks in Germany between 1974 and 2009 by a novel phage-typing scheme. Epidemiol Infect. 2014; 142(1):75-83. 10.1016/j.puhe.2014.02.002 Chironna M, Tafuri S, Gallone MS, Sallustio A, Martinelli D, Prato R, Germinario C, et al. Outbreak of Salmonella Infantis gastroenteritis among people who had eaten at a hash house in southern Italy. Public Health. 2014; 128(5):438-43. 10.1016/j.meegid.2013.07.004 Almeida F, Pitondo-Silva A, Oliveira MA, Falcão JP. Molecular epidemiology and virulence markers of Salmonella Infantis isolated over 25 years in São Paulo State, Brazil. Infect Genet Evol. 2013; 19:145-51. 10.1017/S095026881100197X Bassal R, Reisfeld A, Andorn N, Yishai R, Nissan I, Agmon V, Peled N, Block C, Keller N, Kenes Y, Taran D, Schemberg B, Ken-Dror S, Rouach T, Citron B, Berman E, Green MS, Shohat T, Cohen D, et al. Recent trends in the epidemiology of non-typhoidal Salmonella in Israel, 1999-2009. Epidemiol Infect. 2012; 140(8):1446-53. Instituto Nacional de Salud. EDA microbiología Salm junio 2014. [Internet]. Disponible en: https://goo.gl/DzNBL4. Consultado noviembre de 2014. 10.1111/zph.12081 Velhner M, Kozoderović G, Grego E, Galić N, Stojanov I, Jelesić Z, Kehrenberg C, et al,Clonal spread of Salmonella enterica serovar Infantis in Serbia: acquisition of mutations in the topoisomerase genes gyr A and par C leads to increased resistance to fluoroquinolones. Zoonoses Public Health. 2014; 61(5):364-70. 10.1089/fpd.2011.1038 Hauser E, Tietze E, Helmuth R, Junker E, Prager R, Schroeter A, Rabsch W, Fruth A, Toboldt A, Malorny B, etal. Clonal dissemination of Salmonella enterica serovar Infantis in Germany. Foodborne Pathog Dis. 2012; 9(4):352-60. 10.4315/0362-028X.JFP-13-150 Ferreira V, Wiedmann M, Teixeira P, Stasiewicz MJ. Listeria monocytogenes persistence in food-associated environments: epidemiology, strain characteristics, and implications for public health. J Food Prot. 2014; 77(1):150-70. Muñoz AB, Chaves JA, Rodríguez EC, Realpe ME. Listeria monocytogenes in food handlers: a new approach to address the dangers in food industry.Biomédica. 2013; 33: 283-291. 10.1016/j.foodcont.2012.03.031 GómezD., AriñoA, Carramiñana JJ, Rota C, Yangüela J. Sponge versus mini-roller for the surface microbiological control of Listeria monocytogenes, total aerobic mesophiles and Enterobacteriaceae in the meat industry. Food Control. 2012; 27(1):242–247. 10.1016/j.foodcont.2012.05.013 Neal, J. Comparative analysis of training delivery methods for new employees cleaning and sanitizing retail deli slicers: An exploratory study. Food Control. 2013; 29(1):149–155. 10.1086/429324 Ooi ST, Lorber B. Gastroenteritis due to Listeria monocytogenes. Clin Infect Dis. 2005; 40(9):1327-32. 10.1016/S1473-3099(14)70903-X Leclercq A, Charlier C, Lecuit M. Global burden of listeriosis: the tip of the iceberg. Lancet Infect Dis. 2014; (14):1473-3099 Frye DM, Zweig R, Sturgeon J, Tormey M, LeCavalier M, Lee I, Lawani L, Mascola L et al. An outbreak of febrile gastroenteritis associated with delicatessen meat contaminated with 10.1086/342582 Listeria monocytogenes. Clin Infect Dis. 2002; 35(8):943-9. Carrique-Mas JJ, Hökeberg I, Andersson Y, Arneborn M, Tham W, Danielsson-Tham ML, Osterman B, Leffler M, Steen M, Eriksson E, Hedin G, Giesecke J, et al.Febrile gastroenteritis 10.1017/S0950268802008014 after eating on-farm manufactured fresh cheese--an outbreak of listeriosis? Epidemiol Infect. 2003; 130(1):79-86. Protocolo de vigilancia y control de enfermedades transmitidas por alimentos. Grupo de Factores de Riesgo Ambiental. Instituto Nacional de Salud. 2011. 10.4061/2011/239391 Rahman A, Bonny TS, Stonsaovapak S, Ananchaipattana C. Yersinia enterocolitica: Epidemiological Studies and Outbreaks.J Pathog. 2011; 239391. Park YS, Lee SR, Kim YG. Detection of Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus and Listeria monocytogenes in kimchi by multiplex polymerase chain reaction (mPCR). J Microbiol. 2006; 44(1):92-7. Hayashi M, Natori T, Kubota-Hayashi S, Miyata M, Ohkusu K, Kawamoto K, Kurazono H, Makino S, Ezaki T., et al. A new protocol to detect multiple foodborne pathogens with PCR 10.1155/2013/295050 dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth. Biomed Res Int. 2013; 295050. 10.3310/hta11360 Abubakar I, Irvine L, Aldus CF, Wyatt GM, Fordham R, Schelenz S, et al. A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food. Health Technol Assess 2007; (36):1-216.