{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:21:50Z","timestamp":1767831710677,"version":"3.49.0"},"reference-count":31,"publisher":"Universidad Nacional de Colombia","issue":"6","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. salud p\u00fablica"],"abstract":"<jats:p>Objetivo Caracterizar la microbiota bacteriana presente en los bios\u00f3lidos generados en una de las plantas de tratamiento de aguas residuales m\u00e1s grande de Colombia.Materiales y M\u00e9todos Se utiliz\u00f3 la plataforma de secuenciamiento 454 de la compa\u00f1\u00eda Roche para secuenciar las regiones variables V1-V3 y V6-V9 del marcador molecular 16S rRNA y caracterizar la microbiota. Adicionalmente, se aplicaron estrategias filogen\u00e9ticas para la identificaci\u00f3n de especies bacterianas de importancia.Resultados Nuestros an\u00e1lisis muestran que los Phyla m\u00e1s abundantes son Chloroflexi, Proteobacteria, Bacteroidetes, Actinobacteria y Firmicutes. Los g\u00e9neros clasificados m\u00e1s abundantes fueron Pseudomonas, Dysgonomonas y Proteiniphilum. Sin embargo, el grupo dominante seg\u00fan la regi\u00f3n variable V1-V3 es una Anaerolineaceae que no se ajusta a las especies descritas para esta familia.Conclusiones En las muestras de bios\u00f3lido analizadas predominan bacterias ambientales que participan en los procesos de estabilizaci\u00f3n de la materia org\u00e1nica durante los tratamientos biol\u00f3gicos de tipo secundario y la digesti\u00f3n anaerobia. Se detectaron secuencias de especies dentro de la familia Anaerolineaceae, los an\u00e1lisis filogen\u00e9ticos muestran que probablemente se trata de especies no descritas. En el momento del estudio, se encontr\u00f3 que en el sistema de digesti\u00f3n anaerobia se genera biosolido con una baja carga de bacterias potencialmente pat\u00f3genas.<\/jats:p>","DOI":"10.15446\/rsap.v19n6.67950","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T20:16:32Z","timestamp":1529698592000},"page":"806-813","source":"Crossref","is-referenced-by-count":1,"title":["Descripci\u00f3n de la microbiota bacteriana residente en el bios\u00f3lido generado en la planta de tratamiento de aguas residuales San Fernando. Itag\u00fc\u00ed, Colombia"],"prefix":"10.15446","volume":"19","author":[{"given":"\u00c1ngela J.","family":"Ar\u00e9valo-Arbel\u00e1ez","sequence":"first","affiliation":[]},{"given":"Katherine","family":"Bedoya-Urrego","sequence":"additional","affiliation":[]},{"given":"Felipe","family":"Cabarcas-Jaramillo","sequence":"additional","affiliation":[]},{"given":"Juan Fernando","family":"Alzate-Restrepo","sequence":"additional","affiliation":[]}],"member":"6146","published-online":{"date-parts":[[2017,11,1]]},"reference":[{"key":"ref1","unstructured":"EPM. Plantas de agua EPM [Internet]. Plantas de agua EPM. 2016. Available from: https:\/\/goo.gl\/JZa7JL."},{"key":"ref2","unstructured":"Lozada PT, Escobar JC, P\u00e9rez Vidal A, Imery V R, Nates P, S\u00e1nchez G, et al. Influencia del material de enmienda en el compostaje de lodos de Plantas de Tratamiento de Aguas"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Residuales - PTAR. 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Utilization of sewage sludge in EU application of old and new methods-A review. Renew Sustain Energy Rev. 2008;12(1): 116-40.","DOI":"10.1016\/j.rser.2006.05.014"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Sidhu JPS, Toze SG. Human pathogens and their indicators in biosolids: A literature review. Environ Int. 2009;35(1): 187-201.","DOI":"10.1016\/j.envint.2008.07.006"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Bibby K, Viau E, Peccia J. Pyrosequencing of the 16S rRNA gene to reveal bacterial pathogen diversity in biosolids. Water Res. 2010; 44(14): 4252\u201360.","DOI":"10.1016\/j.watres.2010.05.039"},{"key":"ref10","unstructured":"W\u00e9ry N, Lhoutellier C, Ducray F, Delgen\u00e8s JP, Godon JJ. Behaviour of pathogenic and indicator bacteria during urban wastewater treatment and sludge composting, as revealed"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"by quantitative PCR. Water Res. 2008; 42(1\u20132): 53\u201362.","DOI":"10.1016\/j.watres.2007.06.048"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Yu Z, Garc\u00eda-Gonz\u00e1lez R, Schanbacher FL, Morrison M. Evaluations of different hypervariable regions of archaeal 16S rRNA genes in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis. Appl Environ Microbiol. 2008; 74(3): 889\u201393.","DOI":"10.1128\/AEM.00684-07"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Zhang T, Shao M, Ye L. 454 Pyrosequencing reveals bacterial diversity of avtivated sludge from 14 sewage treatment plants. ISME J. 2012; 6: 1137\u201347.","DOI":"10.1038\/ismej.2011.188"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Jimenez B, Maya C, S\u00e1nchez E, Romero A, Lira L, Barrios JA. Comparison of the quantity and quality of the microbiological content of sludge in countries with low and high content of pathogens. Water Sci Technol. 2002.","DOI":"10.2166\/wst.2002.0278"},{"key":"ref15","unstructured":"Bedoya-Urrego K, Acevedo-Ru\u00edz JM, Pel\u00e1ez-Jaramillo CA, Agudelo-L\u00f3pez S del P. Caracterizaci\u00f3n de bios\u00f3lidos generados en la planta de tratamiento de agua residual San"},{"key":"ref16","unstructured":"Fernando, Itag\u00fc\u00ed (Antioquia, Colombia). Rev. Salud Publica. (Bogot\u00e1) 2013; 15(5): 778\u201390."},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Frank JA, Reich CI, Sharma S, Weisbaum JS, Wilson BA, Olsen GJ. Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol. 2008; 74(8): 2461\u201370.","DOI":"10.1128\/AEM.02272-07"},{"key":"ref18","unstructured":"Muyzer G, De Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"genes coding for 16S rRNA. Appl Environ Microbiol. 1993;59(3):695\u2013700.","DOI":"10.1128\/AEM.59.3.695-700.1993"},{"key":"ref20","unstructured":"Engelbrektson A, Kunin V, Wrighton KC, Zvenigorodsky N, Chen F, Ochman H, et al. Experimental factors affecting PCR-based estimates of microbial species richness and"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"evenness. ISME J. 2010;4(5):642\u20137.","DOI":"10.1038\/ismej.2009.153"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Quince C, Lanzen A, Davenport RJ, Turnbaugh PJ. Removing Noise From Pyrosequenced Amplicons. BMC Bioinformatics. 2011; 12(1): 38.","DOI":"10.1186\/1471-2105-12-38"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, et al. Ribosomal Database Project: Data and tools for high throughput rRNA analysis. Nucleic Acids Res. 2014; 42(D1): 1\u201310.","DOI":"10.1093\/nar\/gkt1244"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Lu X, Zhang X, Wang Z, Huang K, Wang Y, Liang W, et al. Bacterial Pathogens and Community Composition in Advanced Sewage Treatment Systems Revealed by Metagenomics Analysis Based on High-Throughput Sequencing. PLoS One. 2015.","DOI":"10.1371\/journal.pone.0125549"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Klang J, Theuerl S, Szewzyk U, Huth M, T\u00f6lle R, Klocke M. Dynamic variation of the microbial community structure during the long-time mono-fermentation of maize and sugar beet silage. Microb Biotechnol. 2015; 8(5): 764\u201375.","DOI":"10.1111\/1751-7915.12263"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Hofstad T, Olsen I, Eribe ER, Falsen E, Collins MD, Lawson P a. Dysgonomonas gen. nov. to accommodate Dysgonomonas gadei sp. nov., an organism isolated from a human gall bladder, and Dysgonomonas capnocytophagoides (formerly CDC group DF-3). Int J Syst Evol Microbiol. 2000;50(6): 2189\u201395.","DOI":"10.1099\/00207713-50-6-2189"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Lawson PA, Carlson P, Wernersson S, Moore ERB, Falsen E. Dysgonomonas hofstadii sp. nov., isolated from a human clinical source. Anaerobe. 2010; 16(2): 161\u20134.","DOI":"10.1016\/j.anaerobe.2009.06.005"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Pramono AK, Sakamoto M, Iino T, Hongoh Y, Ohkuma M. Dysgonomonas termitidis sp. Nov., isolated from the gut of the subterranean termite Reticulitermes speratus. Int J Syst Evol Microbiol. 2015; 65(2): 681\u20135.","DOI":"10.1099\/ijs.0.070391-0"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Krakat N, Schmidt S, Scherer P. Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage. Bioresour Technol. 2011; 102(10): 5692\u2013701.","DOI":"10.1016\/j.biortech.2011.02.108"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Gagliano MC, Braguglia CM, Petruccioli M, Rossetti S. Ecology and biotechnological potential of the thermophilic fermentative Coprothermobacter spp. FEMS Microbiol Ecol. 2015; 91(5): 1\u201312.","DOI":"10.1093\/femsec\/fiv018"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Peix A, Ramirez-Bahena MH, Velazquez E. Historical evolution and current status of the taxonomy of genus Pseudomonas. 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