{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:16:54Z","timestamp":1781011014139,"version":"3.54.1"},"reference-count":25,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Veterinaria Hungarica"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>Consumption of undercooked or raw pork is considered a significant risk factor for human infection with <jats:italic>Toxoplasma gondii.<\/jats:italic> In this study, we investigated the genetic structure of 18 <jats:italic>T. gondii<\/jats:italic> strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable <jats:italic>T. gondii<\/jats:italic> or <jats:italic>T. gondii<\/jats:italic> DNA. Genotyping was attempted from both pig tissues and mouse brains from the bio-assays using a multiplex multilocus nested polymerase chain reaction\u2013restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6, alt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine <jats:italic>T. gondii<\/jats:italic> isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.<\/jats:p>","DOI":"10.1556\/004.2019.022","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T04:17:16Z","timestamp":1561522636000},"page":"204-211","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":9,"title":["Toxoplasma gondii genotypes circulating in domestic pigs in Serbia"],"prefix":"10.1556","volume":"67","author":[{"given":"Ljiljana","family":"Kuruca","sequence":"first","affiliation":[{"name":"1Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovi\u0107a 8, 21 000 Novi Sad, 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