{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T05:08:33Z","timestamp":1747199313782,"version":"3.40.5"},"reference-count":38,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVet"],"published-print":{"date-parts":[[2020,3,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, the occurrence of <jats:italic>Ixodes<\/jats:italic> (<jats:italic>Pholeoixodes<\/jats:italic>) <jats:italic>kaiseri<\/jats:italic> has been reported for the first time in several European countries, but data on the molecular analysis of this hard tick species are still lacking. Therefore, in this study DNA extracts of 28 <jats:italic>I. kaiseri<\/jats:italic> (collected from dogs and red foxes in Germany, Hungary and Romania) were screened with reverse line blot hybridisation (RLB), PCR and sequencing for the presence of 43 tick-borne pathogens or other members of their families from the categories of Anaplasmataceae, piroplasms, rickettsiae and borreliae. <jats:italic>Rickettsia helvetica<\/jats:italic> DNA was detected in one <jats:italic>I. kaiseri<\/jats:italic> female (from a red fox, Romania), for the first time in this tick species. Six ticks (from red foxes, Romania) contained the DNA of <jats:italic>Babesia vulpes<\/jats:italic>, also for the first time in the case of <jats:italic>I. kaiseri<\/jats:italic>. Molecular evidence of <jats:italic>R. helvetica<\/jats:italic> and <jats:italic>B. vulpes<\/jats:italic> in engorged <jats:italic>I. kaiseri<\/jats:italic> does not prove that this tick species is a vector of the above two pathogens, because they might have been taken up by the ticks from the blood of foxes. In addition, one <jats:italic>I. kaiseri<\/jats:italic> female (from a dog, Hungary) harboured <jats:italic>Babesia<\/jats:italic> sp. badger type-B, identified for the first time in Hungary and Central Europe (i.e. it has been reported previously from Western Europe and China). The latter finding can be explained by either the susceptibility of dogs to <jats:italic>Babesia<\/jats:italic> sp. badger type-B, or by transstadial survival of this piroplasm in <jats:italic>I. kaiseri<\/jats:italic>.<\/jats:p>","DOI":"10.1556\/004.2020.00003","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T08:57:33Z","timestamp":1588928253000},"page":"30-33","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":4,"title":["First broad-range molecular screening of tick-borne pathogens in Ixodes (Pholeoixodes) kaiseri, with special emphasis on piroplasms"],"prefix":"10.1556","volume":"68","author":[{"given":"S\u00e1ndor","family":"Hornok","sequence":"first","affiliation":[{"name":"1Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila D.","family":"S\u00e1ndor","sequence":"additional","affiliation":[{"name":"2Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"F\u00f6ldv\u00e1ri","sequence":"additional","affiliation":[{"name":"1Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary"},{"name":"3Evolutionary Systems Research Group, Centre for Ecological Research, Hungarian Academy of Sciences, Tihany, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela M.","family":"Ionic\u0103","sequence":"additional","affiliation":[{"name":"2Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Silaghi","sequence":"additional","affiliation":[{"name":"4Institute of Infectology, Friedrich-Loeffler-Institute, Insel Riems, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00f3ra","family":"Tak\u00e1cs","sequence":"additional","affiliation":[{"name":"1Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna-margarita","family":"Sch\u00f6tta","sequence":"additional","affiliation":[{"name":"5Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, A-1090, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4559-5960","authenticated-orcid":true,"given":"Michiel","family":"Wijnveld","sequence":"additional","affiliation":[{"name":"5Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, A-1090, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref21","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0304-4017(02)00417-X","article-title":"Ixodes hexagonus is the main candidate as vector of Theileria annae in northwest Spain","volume":"112","author":"Camacho","year":"2003","journal-title":"Vet. 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Epidemiol."},{"key":"ref201","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1017\/S0031182017000476","article-title":"Detection of Babesia DNA in blood and spleen samples from Eurasian badgers (Meles meles) in Scotland","volume":"144","author":"Bartley","year":"2017","journal-title":"Parasitology."},{"key":"ref261","doi-asserted-by":"crossref","first-page":"691","DOI":"10.17236\/sat00087","article-title":"Detection of \u2018Candidatus Neoehrlichia mikurensis\u2019 and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries","volume":"158","author":"Hofmann-Lehmann","year":"2016","journal-title":"Schweiz. Arch. 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Parasitol."},{"key":"ref331","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on tick-borne rickettsioses around the world: a geographic approach","volume":"26","author":"Parola","year":"2013","journal-title":"Clin. Microbiol. 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Open."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1017\/S0031182017000476","article-title":"Detection of Babesia DNA in blood and spleen samples from Eurasian badgers (Meles meles) in Scotland","volume":"144","author":"Bartley","year":"2017","journal-title":"Parasitology."},{"key":"ref131","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.ttbdis.2014.01.005","article-title":"A molecular survey of Babesia spp. and Theileria spp. in red foxes (Vulpes vulpes) and their ticks from Thuringia, Germany","volume":"5","author":"Najm","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"ref291","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1186\/s13071-017-2424-x","article-title":"Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females","volume":"10","author":"Hornok","year":"2017","journal-title":"Parasit. 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