{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:31:54Z","timestamp":1759937514084,"version":"3.40.5"},"reference-count":76,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVet"],"published-print":{"date-parts":[[2020,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objectives of this study were to describe the parameters of dromedary camel epididymal spermatozoa collected by retrograde flushing (RF) technique and to evaluate the freezability of the collected sperm, diluted with and without the supplementation of seminal plasma (SP). Two experiments were conducted: in Experiment 1, ES were recovered within 6\u20138 h after castration; selected samples were diluted with a Tris-citrate egg-yolk glycerolated buffer and frozen. In Experiment 2, epididymides were stored for 24 h at 4 \u00b0C before RF and semen samples were frozen after dilution with a Tris-lactose egg-yolk glycerolated extender with and without 15% SP. In Experiment 1, eight semen samples were obtained from ten epididymides with a mean of 500\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> total spermatozoa recovered, per flushed epididymis. Mean post-thaw motility and progressive motility were 75 and 17%, respectively. In Experiment 2, 15 samples were collected, out of the 18 epididymides (mean number of collected spermatozoa: 700\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>), and 13 of these samples were of excellent quality. Post-thaw parameters were not satisfactory but the supplementation of the freezing medium with 15% SP improved the progressive motility and kinematic parameters of the spermatozoa.<\/jats:p>","DOI":"10.1556\/004.2020.00042","type":"journal-article","created":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T03:00:17Z","timestamp":1604718017000},"page":"289-297","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":4,"title":["Retrograde flushing collection and freezing of dromedary camel epididymal spermatozoa with seminal plasma"],"prefix":"10.1556","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8938-9099","authenticated-orcid":true,"given":"Davide","family":"Monaco","sequence":"first","affiliation":[{"name":"1Department of Veterinary Medicine, University of Bari Aldo Moro, Road to Casamassima km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Batista","sequence":"additional","affiliation":[{"name":"2Reproduction Clinic, Universitary Institute of Biomedical Research, University of Las Palmas Gran Canaria (ULPGC), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Amann","sequence":"additional","affiliation":[{"name":"3Museo del Campo Majorero, Oasis Park, Fuerteventura, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Padalino","sequence":"additional","affiliation":[{"name":"4Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter","family":"Pieters","sequence":"additional","affiliation":[{"name":"3Museo del Campo Majorero, Oasis Park, Fuerteventura, Spain"},{"name":"5Parc Animalier d\u2019Auvergne, Ardes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariacristina","family":"Morelli","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Medicine, University of Bari Aldo Moro, Road to Casamassima km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluca","family":"Accogli","sequence":"additional","affiliation":[{"name":"6Department of Emergency and Transplantation of Organs (D.E.T.O.), University of Bari Aldo Moro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Desantis","sequence":"additional","affiliation":[{"name":"6Department of Emergency and Transplantation of Organs (D.E.T.O.), University of Bari Aldo Moro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni Michele","family":"Lacalandra","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Medicine, University of Bari Aldo Moro, Road to Casamassima km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref81","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.anireprosci.2008.02.001","article-title":"Effects of long-term chilled storage of red deer epididymides on DNA integrity and motility of thawed spermatozoa","volume":"111","author":"Fern\u00e1ndez-Santos","year":"2009","journal-title":"Anim. 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