{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:21:23Z","timestamp":1790486483739,"version":"4.1.0"},"reference-count":90,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVet"],"published-print":{"date-parts":[[2021,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study is the first to investigate the effects of tebuconazole (TEB) on the physiological functions of bovine testicular cells and epididymal spermatozoa. Motility and plasma membrane integrity of spermatozoa exposed to TEB (0.001\u2013100 \u00b5M) were evaluated at different incubation times (0\u20136 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from the parenchyma of bovine testes, were seeded at 1.0 \u00d7 10<jats:sup>4<\/jats:sup>and 1.5 \u00d7 10<jats:sup>6<\/jats:sup>cells\/well in 96- and 12-well culture plates and incubated for 48 h in culture media containing TEB (0.001\u2013100 \u00b5M) to evaluate cytotoxicity and hormone release, respectively. TEB did not affect the motility and plasma membrane integrity. However, significant spermiotoxicity occurred at higher TEB (1\u2013100 \u00b5M) concentrations (<jats:italic>P<\/jats:italic>&lt; 0.05) compared to control and lower doses. Although no dose caused cytotoxicity in testicular cells (<jats:italic>P<\/jats:italic>&gt; 0.05), 1 and 100 \u00b5M TEB caused a significant increase in testosterone secretion (<jats:italic>P<\/jats:italic>&lt; 0.05). As a result, high doses of TEB (1\u2013100 \u00b5M) had slightly suppressive effects on spermatozoa; however, these doses had stimulatory effects on testosterone secretion by testicular cells. It appears that the disruption of hormonal homeostasis of testicular cells after TEB exposure may result in metabolic and especially reproductive adverse effects in bulls.<\/jats:p>","DOI":"10.1556\/004.2021.00023","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T18:41:47Z","timestamp":1625164907000},"page":"180-188","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of tebuconazole exposure on bovine testicular cells and epididymal spermatozoa"],"prefix":"10.1556","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-0933","authenticated-orcid":true,"given":"Ruhi","family":"Kabakci","sequence":"first","affiliation":[{"name":"1Department of Physiology, Faculty of Veterinary Medicine, Kirikkale University, 71450, Kirikkale, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7903-4358","authenticated-orcid":true,"given":"Abdulkadir","family":"Kaya","sequence":"additional","affiliation":[{"name":"2Department of Artificial Insemination and Reproduction, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5837-6877","authenticated-orcid":true,"given":"Ayse Arzu","family":"Yigit","sequence":"additional","affiliation":[{"name":"1Department of Physiology, Faculty of Veterinary Medicine, Kirikkale University, 71450, Kirikkale, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2777-3586","authenticated-orcid":true,"given":"Omer","family":"Varisli","sequence":"additional","affiliation":[{"name":"2Department of Artificial Insemination and Reproduction, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref201","doi-asserted-by":"crossref","first-page":"75","DOI":"10.24880\/maeuvfd.648962","article-title":"The effect of aluminium obtained from natural conditions on in vitro spermatological parameters at low doses in Akkaraman rams [in Turkish]","volume":"5","author":"Kaya","year":"2020","journal-title":"MAE Vet. 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