{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:21:50Z","timestamp":1740140510267,"version":"3.37.3"},"reference-count":56,"publisher":"Akademiai Kiado Zrt.","issue":"2","funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVet"],"published-print":{"date-parts":[[2021,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An adult, mixed-breed, feline leukaemia virus (FeLV-) positive female cat was presented with mucosal jaundice and a history of anorexia and constipation for three days. Physical examination revealed splenomegaly, cachexia, and dehydration. Humane euthanasia was conducted, followed by postmortem examination. Grossly, the cat was icteric, and presented hepatomegaly with multifocal white spots and splenomegaly. Histologically, the bone marrow was nearly completely replaced by a proliferation of megakaryocytes and megakaryoblasts, and there was a proliferation of fibrous connective tissue. Similar neoplastic proliferation was observed infiltrating the liver, lymph nodes, spleen, kidney, skeletal muscle, and lungs. Immunohistochemistry was performed for von Willebrand Factor (VWF), CD79\u03b1, CD3, feline immunodeficiency virus, FeLV, and CD61. Marked cytoplasmic labelling was observed in the neoplastic cells for FeLV, VWF and CD61, corroborating the diagnosis of acute megakaryoblastic leukaemia.<\/jats:p>","DOI":"10.1556\/004.2021.00025","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T10:33:31Z","timestamp":1625481211000},"page":"175-179","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":0,"title":["Pathological and immunohistochemical aspects of acute megakaryoblastic leukaemia in a cat \u2013 Short communication"],"prefix":"10.1556","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7864-6173","authenticated-orcid":true,"given":"Franci\u00e9li Adriane","family":"Molossi","sequence":"first","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luan Cleber","family":"Henker","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca Santana De","family":"Cecco","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcele Bettim","family":"Bandinelli","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rochana","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"2Chatterie Centro de Sa\u00fade do Gato, Private Veterinary Clinic, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana","family":"Sonne","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Driemeier","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saulo Petinatti","family":"Pavarini","sequence":"additional","affiliation":[{"name":"1Department of Veterinary Pathology, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref321","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1939-165X.1996.tb00958.x","article-title":"Acute megakaryoblastic leukemia in a cat","volume":"25","author":"Burton","year":"1996","journal-title":"Vet. Clin. Pathol."},{"key":"ref381","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/oxfordjournals.epirev.a036246","article-title":"Feline leukemia: a naturally occurring cancer of infectious origin","volume":"4","author":"Essex","year":"1982","journal-title":"Epidemiol. Rev."},{"key":"ref301","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cimid.2018.12.004","article-title":"Prevalence of and factors associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) in cats of the state of Santa Catarina, Brazil","volume":"63","author":"Biezus","year":"2019","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref551","first-page":"6988","article-title":"Clinical diagnosis of adult patients with acute megakaryocytic leukemia","volume":"16","author":"Zhao","year":"2018","journal-title":"Onc. Lett."},{"key":"ref221","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1177\/104063870601800311","article-title":"Clinical, histopathological and immunohistochemical findings in a case of megakaryoblastic leukemia in a dog","volume":"18","author":"Park","year":"2006","journal-title":"J. Vet. Diagn. Invest."},{"key":"ref461","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1177\/030098589002700314","article-title":"Identification and characterization of megakaryoblasts in acute megakaryoblastic leukemia in a dog","volume":"27","author":"Messick","year":"1990","journal-title":"Vet. Pathol."},{"key":"ref121","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1182\/blood-2015-05-567859","article-title":"The biology of pediatric acute megakaryoblastic leukemia","volume":"126","author":"Gruber","year":"2015","journal-title":"Blood"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1590\/s0100-736x2017001200028","article-title":"Hematological findings and factors associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) positivity in cats from southern Brazil","volume":"37","author":"Costa","year":"2017","journal-title":"Pesqui. Vet. Bras."},{"key":"ref151","first-page":"8","article-title":"Megakaryocytic leukemia in a cat","volume":"13","author":"Holscher","year":"1983","journal-title":"Feline Pract."},{"key":"ref251","first-page":"679","article-title":"Uber einen Fall von akuter Mega- karyocyblasten-Leukamie, zugleich einige Bemerkungen zum Problem der akuten Leukemie [in German]","volume":"118","author":"Von Boros","year":"1931","journal-title":"Zschr. f\u00fcr Klin. Med."},{"key":"ref191","first-page":"1021","article-title":"Megakaryocytic myelosis in a cat","volume":"168","author":"Michel","year":"1976","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref31","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.cvsm.2016.11.015","article-title":"Feline cholangitis","volume":"47","author":"Boland","year":"2017","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"ref161","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1177\/030098587801500416","article-title":"Megakaryocytic leukemia in a dog","volume":"15","author":"Holscher","year":"1978","journal-title":"Vet. Pathol."},{"key":"ref171","first-page":"1","article-title":"Epidemiological, pathological and immunohistochemical aspects of 125 cases of feline lymphoma in Southern Brazil","volume":"18","author":"Leite-Filho","year":"2019","journal-title":"Vet. Comp. Oncol."},{"key":"ref471","first-page":"1021","article-title":"Megakaryocytic myelosis in a cat","volume":"168","author":"Michel","year":"1976","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref211","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1182\/blood-2005-06-2450","article-title":"Adult acute megakaryocytic leukemia: an analysis of 37 patients treated at M. D. Anderson Cancer Center","volume":"107","author":"Oki","year":"2006","journal-title":"Blood"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1177\/030098589303000212","article-title":"Acute megakaryoblastic leukemia in one cat and two dogs","volume":"30","author":"Colbatzky","year":"1993","journal-title":"Vet. Pathol."},{"key":"ref411","first-page":"941","article-title":"Feline leukemia virus non-neoplastic diseases","volume":"17","author":"Hardy","year":"1981","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref61","doi-asserted-by":"crossref","first-page":"327","DOI":"10.5326\/0460327","article-title":"Acute megakaryoblastic leukemia in dogs: a report of three cases and review of the literature","volume":"46","author":"Comazzi","year":"2010","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref331","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1177\/030098589303000212","article-title":"Acute megakaryoblastic leukemia in one cat and two dogs","volume":"30","author":"Colbatzky","year":"1993","journal-title":"Vet. Pathol."},{"key":"ref451","first-page":"1","article-title":"Epidemiological, pathological and immunohistochemical aspects of 125 cases of feline lymphoma in Southern Brazil","volume":"18","author":"Leite-Filho","year":"2019","journal-title":"Vet. Comp. Oncol."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cimid.2018.12.004","article-title":"Prevalence of and factors associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) in cats of the state of Santa Catarina, Brazil","volume":"63","author":"Biezus","year":"2019","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref201","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1292\/jvms.58.177","article-title":"A case of megakaryoblastic leukemia in a dog","volume":"58","author":"Miyamoto","year":"1996","journal-title":"J. Vet. Med. Sci."},{"key":"ref501","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1177\/104063870601800311","article-title":"Clinical, histopathological and immunohistochemical findings in a case of megakaryoblastic leukemia in a dog","volume":"18","author":"Park","year":"2006","journal-title":"J. Vet. Diagn. Invest."},{"key":"ref371","doi-asserted-by":"crossref","first-page":"149","DOI":"10.5326\/JAAHA-MS-5868","article-title":"AAHA\/AAFP fluid therapy guidelines for dogs and cats","volume":"49","author":"Davis","year":"2013","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref361","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jcpa.2019.05.002","article-title":"Feline leukaemia virus associated with leukaemia in cats in Santa Catarina, Brazil","volume":"170","author":"Cristo","year":"2019","journal-title":"J. Comp. Pathol."},{"key":"ref91","doi-asserted-by":"crossref","first-page":"149","DOI":"10.5326\/JAAHA-MS-5868","article-title":"AAHA\/AAFP fluid therapy guidelines for dogs and cats","volume":"49","author":"Davis","year":"2013","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref101","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/oxfordjournals.epirev.a036246","article-title":"Feline leukemia: a naturally occurring cancer of infectious origin","volume":"4","author":"Essex","year":"1982","journal-title":"Epidemiol. Rev."},{"key":"ref01","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1476-5829.2009.00189.x","article-title":"Clinical pathological and epidemiological assessment of morphologically and immunologically confirmed canine leukaemia","volume":"7","author":"Adam","year":"2009","journal-title":"Vet. Comp. Oncol."},{"key":"ref351","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1590\/s0100-736x2017001200028","article-title":"Hematological findings and factors associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) positivity in cats from southern Brazil","volume":"37","author":"Costa","year":"2017","journal-title":"Pesqui. Vet. Bras."},{"key":"ref421","first-page":"267","article-title":"Disorders of bone marrow","volume-title":"Veterinary Hematology: A Diagnostic Guide and Color Atlas","author":"Harvey","year":"2012"},{"key":"ref131","first-page":"941","article-title":"Feline leukemia virus non-neoplastic diseases","volume":"17","author":"Hardy","year":"1981","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref141","first-page":"267","article-title":"Disorders of bone marrow","volume-title":"Veterinary Hematology: A Diagnostic Guide and Color Atlas","author":"Harvey","year":"2012"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jcpa.2019.05.002","article-title":"Feline leukaemia virus associated with leukaemia in cats in Santa Catarina, Brazil","volume":"170","author":"Cristo","year":"2019","journal-title":"J. Comp. Pathol."},{"key":"ref311","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.cvsm.2016.11.015","article-title":"Feline cholangitis","volume":"47","author":"Boland","year":"2017","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"ref481","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1292\/jvms.58.177","article-title":"A case of megakaryoblastic leukemia in a dog","volume":"58","author":"Miyamoto","year":"1996","journal-title":"J. Vet. Med. Sci."},{"key":"ref181","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1177\/030098589002700314","article-title":"Identification and characterization of megakaryoblasts in acute megakaryoblastic leukemia in a dog","volume":"27","author":"Messick","year":"1990","journal-title":"Vet. Pathol."},{"key":"ref391","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetimm.2008.01.019","article-title":"Molecular pathogenesis of feline leukemia virus-induced malignancies: insertional mutagenesis","volume":"123","author":"Fujino","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref521","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1111\/j.1748-5827.1983.tb00364.x","article-title":"Megakaryocytic myelosis in cats: review and case report","volume":"24","author":"Schmidt","year":"1983","journal-title":"J. Small Anim. Pract."},{"key":"ref111","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetimm.2008.01.019","article-title":"Molecular pathogenesis of feline leukemia virus-induced malignancies: insertional mutagenesis","volume":"123","author":"Fujino","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref431","first-page":"8","article-title":"Megakaryocytic leukemia in a cat","volume":"13","author":"Holscher","year":"1983","journal-title":"Feline Pract."},{"key":"ref341","doi-asserted-by":"crossref","first-page":"327","DOI":"10.5326\/0460327","article-title":"Acute megakaryoblastic leukemia in dogs: a report of three cases and review of the literature","volume":"46","author":"Comazzi","year":"2010","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref281","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1476-5829.2009.00189.x","article-title":"Clinical pathological and epidemiological assessment of morphologically and immunologically confirmed canine leukaemia","volume":"7","author":"Adam","year":"2009","journal-title":"Vet. Comp. Oncol."},{"key":"ref231","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/vcp.12642","article-title":"Thrombocytosis and central nervous system involvement in a case of canine acute megakaryoblastic leukemia","volume":"47","author":"Rochel","year":"2018","journal-title":"Vet. Clin. Pathol."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1177\/1098612X12444693","article-title":"Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro","volume":"14","author":"Almeida","year":"2012","journal-title":"J. Feline Med. Surg."},{"key":"ref41","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1939-165X.1996.tb00958.x","article-title":"Acute megakaryoblastic leukemia in a cat","volume":"25","author":"Burton","year":"1996","journal-title":"Vet. Clin. Pathol."},{"key":"ref241","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1111\/j.1748-5827.1983.tb00364.x","article-title":"Megakaryocytic myelosis in cats: review and case report","volume":"24","author":"Schmidt","year":"1983","journal-title":"J. Small Anim. Pract."},{"key":"ref261","first-page":"203","article-title":"Tumors of the hemolymphatic system","volume-title":"Tumors in Domestic Animals","author":"Valli","year":"2017"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1182\/blood-2015-05-567859","article-title":"The biology of pediatric acute megakaryoblastic leukemia","volume":"126","author":"Gruber","year":"2015","journal-title":"Blood"},{"key":"ref271","first-page":"6988","article-title":"Clinical diagnosis of adult patients with acute megakaryocytic leukemia","volume":"16","author":"Zhao","year":"2018","journal-title":"Onc. Lett."},{"key":"ref441","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1177\/030098587801500416","article-title":"Megakaryocytic leukemia in a dog","volume":"15","author":"Holscher","year":"1978","journal-title":"Vet. Pathol."},{"key":"ref531","first-page":"679","article-title":"Uber einen Fall von akuter Mega- karyocyblasten-Leukamie, zugleich einige Bemerkungen zum Problem der akuten Leukemie [in German]","volume":"118","author":"Von Boros","year":"1931","journal-title":"Zschr. f\u00fcr Klin. Med."},{"key":"ref511","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/vcp.12642","article-title":"Thrombocytosis and central nervous system involvement in a case of canine acute megakaryoblastic leukemia","volume":"47","author":"Rochel","year":"2018","journal-title":"Vet. Clin. Pathol."},{"key":"ref291","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1177\/1098612X12444693","article-title":"Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro","volume":"14","author":"Almeida","year":"2012","journal-title":"J. Feline Med. Surg."},{"key":"ref491","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1182\/blood-2005-06-2450","article-title":"Adult acute megakaryocytic leukemia: an analysis of 37 patients treated at M. D. Anderson Cancer Center","volume":"107","author":"Oki","year":"2006","journal-title":"Blood"},{"key":"ref541","first-page":"203","article-title":"Tumors of the hemolymphatic system","volume-title":"Tumors in Domestic Animals","author":"Valli","year":"2017"}],"container-title":["Acta Veterinaria Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/69\/2\/article-p175.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/004\/69\/2\/article-p175.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T23:34:05Z","timestamp":1640043245000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/69\/2\/article-p175.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,31]]},"references-count":56,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/004.2021.00025","relation":{},"ISSN":["0236-6290","1588-2705"],"issn-type":[{"type":"print","value":"0236-6290"},{"type":"electronic","value":"1588-2705"}],"subject":[],"published":{"date-parts":[[2021,7,31]]}}}