{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T06:48:44Z","timestamp":1776494924779,"version":"3.51.2"},"reference-count":58,"publisher":"Akademiai Kiado Zrt.","issue":"2","license":[{"start":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T00:00:00Z","timestamp":1627689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lend\u00fclet program","award":["LP2012-22"],"award-info":[{"award-number":["LP2012-22"]}]},{"DOI":"10.13039\/501100003825","name":"Hungarian Academy of Sciences","doi-asserted-by":"crossref","award":["K16 (119594)"],"award-info":[{"award-number":["K16 (119594)"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003825","name":"Hungarian Academy of Sciences","doi-asserted-by":"crossref","award":["FK17 (124019)"],"award-info":[{"award-number":["FK17 (124019)"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003825","name":"Hungarian Academy of Sciences","doi-asserted-by":"crossref","award":["KKP19 (129751)"],"award-info":[{"award-number":["KKP19 (129751)"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Research, Development and Innovation Office"},{"DOI":"10.13039\/501100003825","name":"Hungarian Academy of Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"crossref"}]},{"name":"New National Excellence Program of the Ministry of Innovation and Technology","award":["\u00daNKP-19-4-\u00c1TE-1"],"award-info":[{"award-number":["\u00daNKP-19-4-\u00c1TE-1"]}]},{"name":"New National Excellence Program of the Ministry of Innovation and Technology","award":["\u00daNKP-20-3-I-\u00c1TE-4"],"award-info":[{"award-number":["\u00daNKP-20-3-I-\u00c1TE-4"]}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVet"],"published-print":{"date-parts":[[2021,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The control of <jats:italic>Mycoplasma hyorhinis<\/jats:italic> infection relies mainly on antimicrobial therapy. However, the antibiotic susceptibility testing of the bacteria is usually not performed before applying the treatment, and thus therapeutic failures are not uncommon. In the case of <jats:italic>M. hyorhinis<\/jats:italic>, several antibiotic-resistance-related single nucleotide polymorphisms (SNPs) are known but assays for their detection have not been described yet. The aims of the present study were to investigate macrolide- and lincomycin-resistance-related SNPs in Hungarian <jats:italic>M. hyorhinis<\/jats:italic> isolates and to develop mismatch amplification mutation assays (MAMA) to detect the identified resistance markers. Minimal inhibitory concentrations (MIC) of different drugs and whole genome sequences of 37 <jats:italic>M. hyorhinis<\/jats:italic> isolates were used to find the resistance-related mutations. One MAMA assay was designed to detect the mutation of the 23S rRNA gene at nucleotide position 2058 (<jats:italic>Escherichia coli<\/jats:italic> numbering). For further evaluation, the assay was challenged with 17 additional isolates with available MIC data and 15 DNA samples from clinical specimens. The genotypes of the samples were in line with the MIC test results. The developed assay supports the practice of targeted antibiotic usage; hence it may indirectly reduce some bacterial resistance-related public health concerns.<\/jats:p>","DOI":"10.1556\/004.2021.00026","type":"journal-article","created":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T15:17:16Z","timestamp":1626707836000},"page":"110-115","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":10,"title":["Development of a molecular biological assay for the detection of markers related to decreased susceptibility to macrolides and lincomycin in Mycoplasma hyorhinis"],"prefix":"10.1556","volume":"69","author":[{"given":"Dorottya","family":"F\u00f6ldi","sequence":"first","affiliation":[{"name":"1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hung\u00e1ria krt. 21, H-1143, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsa","family":"Kreizinger","sequence":"additional","affiliation":[{"name":"1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hung\u00e1ria krt. 21, H-1143, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katinka","family":"Bek\u0151","sequence":"additional","affiliation":[{"name":"1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hung\u00e1ria krt. 21, H-1143, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolett","family":"Belecz","sequence":"additional","affiliation":[{"name":"1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hung\u00e1ria krt. 21, H-1143, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kriszti\u00e1n","family":"B\u00e1nyai","sequence":"additional","affiliation":[{"name":"1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hung\u00e1ria krt. 21, H-1143, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kriszti\u00e1n","family":"Kiss","sequence":"additional","affiliation":[{"name":"2SCG Diagnostics Ltd., D\u00e9legyh\u00e1za, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imre","family":"Biksi","sequence":"additional","affiliation":[{"name":"3Department of Pathology, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5698-5713","authenticated-orcid":true,"given":"Mikl\u00f3s","family":"Gyuranecz","sequence":"additional","affiliation":[{"name":"1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hung\u00e1ria krt. 21, H-1143, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref401","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1128\/AAC.41.9.2037","article-title":"Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones","volume":"41","author":"Hannan","year":"1997","journal-title":"Antimicrob. 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Res."},{"key":"ref201","first-page":"285","article-title":"Associations among Haemophilus parasuis, Mycoplasma hyorhinis and porcine reproductive and respiratory syndrome virus infections in pigs with polyserositis","volume":"56","author":"Palzer","year":"2015","journal-title":"Can. Vet. J."},{"key":"ref271","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1128\/AAC.35.2.228","article-title":"Comparison of methods for in vitro testing of susceptibility of porcine Mycoplasma species to antimicrobial agents","volume":"35","author":"Ter Laak","year":"1991","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref451","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1292\/jvms.67.795","article-title":"Macrolides and lincomycin susceptibility of Mycoplasma hyorhinis and variable mutation of domain II and V in 23S ribosomal RNA","volume":"67","author":"Kobayashi","year":"2005","journal-title":"J. Vet. Med. Sci."},{"key":"ref571","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1128\/AAC.35.2.228","article-title":"Comparison of methods for in vitro testing of susceptibility of porcine Mycoplasma species to antimicrobial agents","volume":"35","author":"Ter Laak","year":"1991","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref111","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jcpa.2010.01.012","article-title":"An investigation of the pathology and pathogens associated with porcine respiratory disease complex in Denmark","volume":"143","author":"Hansen","year":"2010","journal-title":"J. Comp. Pathol."},{"key":"ref481","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.vetimm.2016.06.008","article-title":"Mycoplasma hyorhinis is a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus","volume":"177","author":"Lee","year":"2016","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref211","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.tvjl.2014.02.028","article-title":"Macrolides and lincosamides in cattle and pigs: Use and development of antimicrobial resistance","volume":"200","author":"Py\u00f6r\u00e4l\u00e4","year":"2014","journal-title":"Vet. J."},{"key":"ref91","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1051\/vetres:2000100","article-title":"Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary Mycoplasma species","volume":"31","author":"Hannan","year":"2000","journal-title":"Vet. 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Sci."},{"key":"ref551","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AAC.01983-16","article-title":"Mutations associated with decreased susceptibility to seven antimicrobial families in field and laboratory-derived Mycoplasma bovis strains","volume":"61","author":"Sulyok","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref411","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jcpa.2010.01.012","article-title":"An investigation of the pathology and pathogens associated with porcine respiratory disease complex in Denmark","volume":"143","author":"Hansen","year":"2010","journal-title":"J. Comp. Pathol."},{"key":"ref121","doi-asserted-by":"crossref","first-page":"109","DOI":"10.14405\/kjvr.2016.56.2.109","article-title":"In vitro antibiotic susceptibility of field isolates of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis from Korea","volume":"56","author":"Jang","year":"2016","journal-title":"Korean J. Vet. Res."},{"key":"ref541","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetmic.2017.11.026","article-title":"Development of molecular methods for the rapid detection of antibiotic susceptibility of Mycoplasma bovis","volume":"213","author":"Sulyok","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref521","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12917-020-02512-2","article-title":"Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe","volume":"16","author":"Rosales","year":"2020","journal-title":"BMC Vet. Res."},{"key":"ref361","doi-asserted-by":"crossref","first-page":"108697","DOI":"10.1016\/j.vetmic.2020.108697","article-title":"Development of molecular biological tools for the rapid determination of antibiotic susceptibility of Mycoplasma hyopneumoniae isolates","volume":"245","author":"Felde","year":"2020","journal-title":"Vet. 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