{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T10:11:32Z","timestamp":1756894292757},"reference-count":60,"publisher":"Akademiai Kiado Zrt.","issue":"4","license":[{"start":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T00:00:00Z","timestamp":1638403200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVet"],"published-print":{"date-parts":[[2021,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cotyledons (<jats:italic>n<\/jats:italic>= 167) from 30 Hungarian and 5 Slovakian dairy cattle herds were analysed for<jats:italic>Coxiella burnetii<\/jats:italic>by real-time PCR targeting the<jats:italic>IS1111<\/jats:italic>gene. Eighty (88.9%) out of the 90 cotyledons from retained placentas and 31 (40.3%) out of the 77 cotyledons from normally separated placentas tested positive. Seventeen out of the 80 positive samples (21.3%) originating from retained placentas were found to be highly loaded with<jats:italic>C. burnetii<\/jats:italic>with a cycle threshold (Ct) value lower than or equal to 27.08, ranging between 11.92 and 27.08. The rest of the positive samples from retained fetal membranes and from normally separated placentas were moderately loaded with<jats:italic>C. burnetii<\/jats:italic>DNA. Five out of the ten samples showing the strongest positivity (Ct 11.92\u201318.28) from retained placentas were genotyped by multispacer sequence typing based on ten loci, which revealed sequence type (ST) 61, a type that had not been detected in Hungary and Slovakia previously. Retained placenta was more likely in cows with<jats:italic>C. burnetii<\/jats:italic>PCR-positive cotyledons (odds ratio: 12.61,<jats:italic>P<\/jats:italic>= 0.0023). The high<jats:italic>C. burnetii<\/jats:italic>DNA load found in retained fetal membranes may be a potential risk factor for human infection and may also be associated with the retention of fetal membranes.<\/jats:p>","DOI":"10.1556\/004.2021.00047","type":"journal-article","created":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T15:03:48Z","timestamp":1636038228000},"page":"303-307","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":3,"title":["Prevalence of Coxiella burnetii in bovine placentas in Hungary and Slovakia: Detection of a novel sequence type \u2013 Short communication"],"prefix":"10.1556","volume":"69","member":"425","reference":[{"key":"ref301","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1751-0147-55-13","article-title":"Coxiella burnetii associated reproductive disorders in domestic animals \u2013 a critical review","volume":"55","author":"Agerholm","year":"2013","journal-title":"Acta Vet. 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