{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T18:59:01Z","timestamp":1772650741132,"version":"3.50.1"},"reference-count":10,"publisher":"Akademiai Kiado Zrt.","issue":"1","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVET"],"published-print":{"date-parts":[[2026,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>For many years, artificial insemination (AI) has been regarded as one of the most significant assisted reproductive techniques.<\/jats:p>\n                  <jats:p>The objective of our manuscript is to summarise the preliminary results of the program in which AI with fresh and frozen semen was being used to support the reproduction of a foxhound colony with valuable genetic properties. In this manuscript, three AI cases are presented, one with fresh and two with frozen semen.<\/jats:p>\n                  <jats:p>\n                    Artificial insemination with fresh semen was conducted two days after the LH peak, when the progesterone (P4) concentration exceeded 5\u2009ng*mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Transcervical AI (TCI) was repeated 48\u2009h later.\n                  <\/jats:p>\n                  <jats:p>\n                    Artificial insemination with frozen semen were carried out when P4 level was &gt;15\u2009ng*mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    and TCI was repeated on the next day (Day 5 and 6 after LH peak).\n                  <\/jats:p>\n                  <jats:p>Our preliminary results show that an acceptable litter size can be achieved after TCI with freshly collected and cryopreserved semen. Healthy puppies were successfully created from genetically valuable canine parents. We obtained three pregnancies and 18 offspring in a one-year period. Our data indicate that the technology used (timing of AI, semen handling, TCI technique) could have played a major role in achieving these results.<\/jats:p>","DOI":"10.1556\/004.2025.01227","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T11:16:06Z","timestamp":1764069366000},"page":"55-57","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Reproductive support of a valuable canine colony with assisted reproductive technique \u2013 Short communication"],"prefix":"10.1556","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6136-6841","authenticated-orcid":true,"given":"M\u00f3nika","family":"Bacsa","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f3ra","family":"T\u00f6r\u00f6k","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kriszti\u00e1n","family":"Erdei","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter","family":"Szil\u00e1gyi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e1ndor","family":"Cseh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"B1","series-title":"Small Animal Theriogenology. Veterinary Clinic of North America: Small Animal Practice","year":"2018","unstructured":"Christensen, B. W. (ed) (2018): Small Animal Theriogenology. Veterinary Clinic of North America: Small Animal Practice. Elsevier Inc. ISSN 0195 5616. www.vetsmall.theclinics.com."},{"key":"B2","series-title":"Paper Presented at: ESAVS-EVSSAR-ENVN Reproduction in Companion, Exotic and Laboratory Animal","article-title":"Artificial insemination","author":"Linde-Forsberg, C.","year":"2005","unstructured":"Linde-Forsberg, C. (2005): Artificial insemination. Paper Presented at: ESAVS-EVSSAR-ENVN Reproduction in Companion, Exotic and Laboratory Animal. Nantes. https:\/\/www.canirep.com\/wp-content\/uploads\/2021\/05\/Canine-AI-state-of-the-art.pdf."},{"key":"B3","series-title":"Theriogenology","first-page":"844","article-title":"Comparison of endoscopic-assisted transcervical and laparotomy insemination with frozen-thawed dog semen: a retrospective clinical study","volume":"82","author":"Mason, S. J.","year":"2014","unstructured":"Mason, S. J. and Rous, N. R. (2014): Comparison of endoscopic-assisted transcervical and laparotomy insemination with frozen-thawed dog semen: a retrospective clinical study. Theriogenology 82, 844\u2013850. https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0093691X14003094."},{"key":"B4","series-title":"Theriogenology","first-page":"1029","article-title":"Single, transcervical insemination using frozen-thawed semen in Greyhound: a case series study","volume":"65","author":"Pretzer, S. D.","year":"2006","unstructured":"Pretzer, S. D., Lillich, R. K. and Althouse, G. C. (2006): Single, transcervical insemination using frozen-thawed semen in Greyhound: a case series study. Theriogenology 65, 1029\u20131036. https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0093691X05003092."},{"key":"B5","series-title":"J. Reprod. Fertil. 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Paper Presented at: ESAVS-EVSSAR-ENVN Reproduction in Companion, Exotic and Laboratory Animal. Nantes. https:\/\/www.canirep.com\/wp-content\/uploads\/2021\/05\/Canine-AI-state-of-the-art.pdf."},{"key":"B3","series-title":"Theriogenology","first-page":"844","article-title":"Comparison of endoscopic-assisted transcervical and laparotomy insemination with frozen-thawed dog semen: a retrospective clinical study","volume":"82","author":"Mason, S. J.","year":"2014","unstructured":"Mason, S. J. and Rous, N. R. (2014): Comparison of endoscopic-assisted transcervical and laparotomy insemination with frozen-thawed dog semen: a retrospective clinical study. Theriogenology 82, 844\u2013850. https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0093691X14003094."},{"key":"B4","series-title":"Theriogenology","first-page":"1029","article-title":"Single, transcervical insemination using frozen-thawed semen in Greyhound: a case series study","volume":"65","author":"Pretzer, S. 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