{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:02:51Z","timestamp":1789570971698,"version":"build-2803163510"},"reference-count":40,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["www.akjournals.com"],"crossmark-restriction":true},"short-container-title":["AVET"],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Tilapinevirus tilapiae<\/jats:italic>\n                    (TiLV) (formerly Tilapia Lake Virus) is a highly prevalent pathogen capable of inducing significant mortality rates in Nile tilapia (\n                    <jats:italic>Oreochromis niloticus<\/jats:italic>\n                    ). The alterations induced by Tilapia Lake Virus (TiLV) in the gut microbiota composition, diversity and functional prediction of Nile tilapia have not been thoroughly investigated. This study investigated the gut microbiota of a healthy control group and a TiLV-infected group of Nile tilapia of size 30 \u00b1 2.45g. The alpha diversity of microbiota was hardly affected by TiLV infection, whereas species richness and beta diversity patterns explained the significant differences between control and TiLV-infected groups. The study highlighted a decline in\n                    <jats:italic>Cetobacterium<\/jats:italic>\n                    and an increase in\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    in the TiLV-infected group. The phyla, including Firmicutes, Actinobacteriota and Proteobacteria, exhibited a significant increase in the TiLV-infected\u00a0group, while Bacteroidota and Fusobacteriota decreased substantially. The PICRUSt-based functional gene prediction revealed that TiLV infection had considerably changed the KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathways associated with membrane transport, amino acid metabolism, transcription, carbohydrate metabolism, cellular process and signalling in the gut microbiota of Nile tilapia. The results suggest that the infection by TiLV altered the composition and functional pathways in the gut microbiota of Nile tilapia.\n                  <\/jats:p>","DOI":"10.1556\/004.2026.01216","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:44:54Z","timestamp":1784547894000},"page":"213-225","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Tilapinevirus tilapiae (TiLV) infection on the composition and functional dynamics of\u00a0gut microbiota in Nile Tilapia (Oreochromis niloticus) (Linnaeus, 1758)"],"prefix":"10.1556","volume":"74","author":[{"given":"Asha","family":"Gopan","sequence":"first","affiliation":[{"name":"Kerala University of Fisheries and Ocean Studies Panangad, Kochi 682506, INDIA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8451-0742","authenticated-orcid":true,"given":"Riji John","family":"Kollanoor","sequence":"additional","affiliation":[{"name":"Kerala University of Fisheries and Ocean Studies Panangad, Kochi 682506, INDIA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"B1","series-title":"The\u00a0Scientific World J.","first-page":"931","article-title":"The bacterial microflora of fish, revised","volume":"6","author":"Austin, B.","year":"2006","unstructured":"Austin, B. (2006): The bacterial microflora of fish, revised. The\u00a0Scientific World J. 6, 931\u2013945."},{"issue":"2","key":"B2","series-title":"mBio","first-page":"10","article-title":"Characterization of a novel orthomyxo-like virus causing mass die-offs of tilapia","volume":"7","author":"Bacharach, E.","year":"2016","unstructured":"Bacharach, E., Mishra, N., Briese, T., Zody, M. C., Kembou Tsofack, J. E., Zamostiano, R., Berkowitz, A., Ng, J., Nitido, A., Corvelo, A. and Toussaint, N. C. (2016): Characterization of a novel orthomyxo-like virus causing mass die-offs of tilapia. mBio 7(2), 10\u20131128."},{"issue":"1","key":"B3","series-title":"Cell","first-page":"121","article-title":"Role of the microbiota in immunity and inflammation","volume":"157","author":"Belkaid, Y.","year":"2014","unstructured":"Belkaid, Y. and Hand, T. W. (2014): Role of the microbiota in immunity and inflammation. Cell 157(1), 121\u2013141."},{"issue":"3","key":"B4","series-title":"Biology","first-page":"180","article-title":"The impact of sampling season and catching site (wild and aquaculture) on gut microbiota composition and diversity of Nile tilapia (Oreochromis niloticus)","volume":"10","author":"Bereded, N. K.","year":"2021","unstructured":"Bereded, N. K., Abebe, G. B., Fanta, S. W., Curto, M., Waidbacher, H., Meimberg, H. and Domig, K. J. (2021): The impact of sampling season and catching site (wild and aquaculture) on gut microbiota composition and diversity of Nile tilapia (Oreochromis niloticus). Biology 10(3), 180."},{"issue":"7","key":"B5","series-title":"Microorganisms","first-page":"1040","article-title":"Metabarcoding analyses of gut microbiota of Nile tilapia (Oreochromis niloticus) from Lake Awassa and Lake Chamo, Ethiopia","volume":"8","author":"Bereded, N. K.","year":"2020","unstructured":"Bereded, N. K., Curto, M., Domig, K. J., Abebe, G. B., Fanta, S. W., Waidbacher, H. and Meimberg, H. (2020): Metabarcoding analyses of gut microbiota of Nile tilapia (Oreochromis niloticus) from Lake Awassa and Lake Chamo, Ethiopia. Microorganisms 8(7), 1040."},{"issue":"1","key":"B6","series-title":"Anim. Microbiome","first-page":"1","article-title":"Salmon gut microbiota correlates with disease infection status: potential for monitoring health in farmed animals","volume":"3","author":"Bozzi, D.","year":"2021","unstructured":"Bozzi, D., Rasmussen, J. A., Car\u00f8e, C., Sveier, H., Nord\u00f8y, K., Gilbert, M. T. P. and Limborg, M. T. (2021): Salmon gut microbiota correlates with disease infection status: potential for monitoring health in farmed animals. Anim. Microbiome 3(1), 1\u201317."},{"key":"B7","series-title":"Front. Endocrinol.","article-title":"Gut microbiota and energy homeostasis in fish","volume":"10","author":"Butt, R. L.","year":"2019","unstructured":"Butt, R. L. and Volkoff, H. (2019): Gut microbiota and energy homeostasis in fish. Front. Endocrinol. 10, 429202."},{"key":"B8","series-title":"Front. Cell. Infect. Microbiol.","article-title":"Cyprinid herpesvirus 2 infection changes microbiota and metabolites in the gibel carp (Carassius auratus gibelio) midgut","volume":"12","author":"Chen, P.","year":"2023","unstructured":"Chen, P., Zhang, M., Zhang, Y., Li, J., Wan, X., Lv, T., Chen, Y., Zhao, Z., Ma, Z., Zhu, Z. and Chen, L., (2023): Cyprinid herpesvirus 2 infection changes microbiota and metabolites in the gibel carp (Carassius auratus gibelio) midgut. Front. Cell. Infect. Microbiol. 12, 1017165."},{"key":"B10","series-title":"Anesthetics in aquaculture","volume":"3900","author":"Coyle, S. D.","year":"2004","unstructured":"Coyle, S. D., Durborow, R. M. and Tidwell, J. H., (2004): Anesthetics in aquaculture. (Vol. 3900). Texas: Southern Regional Aquaculture Center."},{"key":"B11","series-title":"GigaScience","first-page":"1","article-title":"Inferring microbiota functions from taxonomic genes: a review","volume":"11","author":"Djemiel, C.","year":"2022","unstructured":"Djemiel, C., Maron, P. A., Terrat, S., Dequiedt, S., Cottin, A. and Ranjard, L. (2022): Inferring microbiota functions from taxonomic genes: a review, GigaScience 11, 1\u201330."},{"key":"B12","series-title":"Aquaculture","first-page":"111","article-title":"Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection","volume":"476","author":"Dong, H. T.","year":"2017","unstructured":"Dong, H. T., Siriroob, S., Meemetta, W., Santimanawong, W., Gangnonngiw, W., Pirarat, N., Khunrae, P., Rattanarojpong, T., Vanichviriyakit, R. and Senapin, S. (2017): Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection. Aquaculture 476, 111\u2013118."},{"key":"B13","series-title":"J. Clin. Microbiol.","first-page":"4137","article-title":"Identification of a novel RNA virus lethal to tilapia","volume":"52","author":"Eyngor, M.","year":"2014","unstructured":"Eyngor, M., Zamostiano, R., Tsofack, J. E. K., Berkowitz, A., Bercovier, H., Tinman, S., Lev, M., Hurvitz, A., Galeotti, M., Bacharach, E. and Eldar, A. (2014): Identification of a novel RNA virus lethal to tilapia. J. Clin. Microbiol. 52, 4137\u20134146."},{"key":"B14","article-title":"Cultured Aquatic Species Information Programme Oreochromis niloticus (Linnaeus, 1758)","author":"FAO","year":"2019","unstructured":"FAO. (2019): Cultured Aquatic Species Information Programme Oreochromis niloticus (Linnaeus, 1758). FAO, Rome. http:\/\/www.fao.org\/fishery\/culturedspecies\/Oreochromis_niloticus\/en."},{"key":"B15","article-title":"The state of world fisheries and aquaculture 2024: Blue transformation in action","author":"FAO","year":"2024","unstructured":"FAO. (2024): The state of world fisheries and aquaculture 2024: Blue transformation in action. FAO, Rome. https:\/\/openknowledge.fao.org\/items\/8ab20ccf-1e9d-4ae6-836c-ca770d16da01."},{"key":"B16","series-title":"Signal Transduction Targeted Ther.","first-page":"135","article-title":"Microbiota in health and diseases","volume":"7","author":"Hou, K.","year":"2022","unstructured":"Hou, K., Wu, Z. X., Chen, X. Y., et\u00a0al. (2022): Microbiota in health and diseases. Signal Transduction Targeted Ther., 7, 135."},{"issue":"10","key":"B17","series-title":"Aquaculture Res.","first-page":"4574","article-title":"A primer on metagenomics and next\u2010generation sequencing in fish gut microbiome research","volume":"52","author":"Johny, T. 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J. Gen. Virol. 104(10), 001903."},{"issue":"9","key":"B20","series-title":"Nat. Biotechnol.","first-page":"814","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille, M. G.","year":"2013","unstructured":"Langille, M. G., Zaneveld, J., Caporaso, J. G., McDonald, D., Knights, D., Reyes, J. A., Clemente, J. C., Burkepile, D. E., Vega Thurber, R. L., Knight, R. and Beiko, R. G. (2013): Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat. Biotechnol. 31(9), 814\u2013821."},{"key":"B21","series-title":"Microb. Ecol.","first-page":"510","article-title":"Bacterial signatures of \u201cred-operculum\u201d disease in the gut of crucian carp (Carassius auratus)","volume":"74","author":"Li, T.","year":"2017","unstructured":"Li, T., Li, H., Gatesoupe, F. J., She, R., Lin, Q., Yan, X., Li, J. and Li, X. 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(2022): Relationships between pond water and tilapia skin microbiomes in aquaculture ponds in Malawi. Aquaculture 558, 738367."},{"issue":"2","key":"B24","series-title":"Acta Sci. Biol Sci.","first-page":"267","article-title":"Bacterial microflora in the gastrointestinal tract of Nile tilapia, Oreochromis niloticus, cultured in a semi-intensive system","volume":"25","author":"Molinari, L. M.","year":"2003","unstructured":"Molinari, L. M., Scoaris, D. D. O., Pedroso, R. B., Bittencourt, N. L. R., Nakamura, C. V., Nakamura, T. U., Abreu Filho, B. D. and Dias Filho, B. P. (2003): Bacterial microflora in the gastrointestinal tract of Nile tilapia, Oreochromis niloticus, cultured in a semi-intensive system. Acta Sci. Biol Sci. 25(2), 267\u2013271."},{"issue":"2","key":"B25","series-title":"Rev.\u00a0Aquacult.","first-page":"579","article-title":"Fish disease prevention via microbial dysbiosis\u2010associated biomarkers in aquaculture","volume":"15","author":"Mougin, J.","year":"2023","unstructured":"Mougin, J. and Joyce, A. (2023) Fish disease prevention via microbial dysbiosis\u2010associated biomarkers in aquaculture. Rev.\u00a0Aquacult. 15(2), 579\u2013594."},{"key":"B26","article-title":"Published by National fisheries development board (Department of animal Husbandry, diary & fisheries, Ministry of Agriculture and Farmers Welfare, Govt. Of India)- guidelines for responsible farming of tilapia in India","author":"NFDB","year":"2015","unstructured":"NFDB. (2015): Published by National fisheries development board (Department of animal Husbandry, diary & fisheries, Ministry of Agriculture and Farmers Welfare, Govt. Of India)- guidelines for responsible farming of tilapia in India. 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(2024): Tilapia lake virus. https:\/\/www.woah.org\/en\/document\/infection-with-tilapia-lake-virus-tilv\/."},{"key":"B29","series-title":"Aquaculture","first-page":"740","article-title":"Tilapia lake virus infection disrupts the gut microbiota of red hybrid tilapia (Oreochromis spp.)","volume":"586","author":"Paimeeka, S.","year":"2024","unstructured":"Paimeeka, S., Tangsongcharoen, C., Lertwanakarn, T., Setthawong, P., Bunkhean, A., Tangwattanachuleeporn, M. and Surachetpong, W. (2024): Tilapia lake virus infection disrupts the gut microbiota of red hybrid tilapia (Oreochromis spp.). Aquaculture 586, 740-752."},{"issue":"3","key":"B30","series-title":"Plos One","article-title":"Salmonid alphavirus infection causes skin dysbiosis in Atlantic salmon (Salmo salar L.) post-smolts","volume":"12","author":"Reid, K. M.","year":"2017","unstructured":"Reid, K. M., Patel, S., Robinson, A. J., Bu, L., Jarungsriapisit, J., Moore, L. J. and Salinas, I. (2017): Salmonid alphavirus infection causes skin dysbiosis in Atlantic salmon (Salmo salar L.) post-smolts. Plos One 12(3), e0172856."},{"issue":"8","key":"B31","series-title":"Aquaculture Res.","first-page":"3412","article-title":"Comparison between the intestinal microbiome of healthy fish and fish experimentally infected with Streptococcus agalactiae","volume":"51","author":"Silva, B. R. D. S.","year":"2020","unstructured":"Silva, B. R. D. S., Derami, M. S., Paix\u00e3o, D. A., Persinoti, G. F., Dias da Silveira, W. and Maluta, R. P. (2020): Comparison between the intestinal microbiome of healthy fish and fish experimentally infected with Streptococcus agalactiae. Aquaculture Res. 51(8), 3412\u20133420."},{"key":"B32","series-title":"World J. Microbiol. Biotechnol.","first-page":"1","article-title":"Altered gut microbiota associated with intestinal disease in grass carp (Ctenopharyngodon idellus)","volume":"34","author":"Tran, N. T.","year":"2018","unstructured":"Tran, N. 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