{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T21:11:04Z","timestamp":1686172264706},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"4","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Veterinaria Hungarica"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>At abattoirs and farms, 1248 sera were collected from animals representing 121 farms, and examined by complement fixation test using <jats:italic>Mycoplasma mycoides<\/jats:italic> subspecies <jats:italic>mycoides<\/jats:italic> small colony type (MmmSC) antigen. All sera were negative except seven from four farms, giving ++ reactions in the serum dilution of 1:10. On retesting, these sera and additional 30 sera collected repeatedly in both farms gave negative results. In isolation attempts, 953 lung samples collected from slaughtered cattle at the same abattoirs, and 326 nasal swabs collected from 11 herds proved to be negative for the presence of MmmSC, but <jats:italic>M. bovis<\/jats:italic> was isolated frequently. In the small farms 23.95% of the animals had pleurisy and\/or pneumonia while in the large herds 34.69% had lesions. DNA extracted from 50 nasal swabs and 430 lung samples was examined by polymerase chain reaction (PCR) using <jats:italic>M. mycoides<\/jats:italic> cluster-specific primers. DNA from further 325 lung samples was tested by the more specific <jats:italic>M. mycoides<\/jats:italic> subspecies <jats:italic>mycoides<\/jats:italic> small colony\/large colony\/capri specific primers and 196 samples by nested PCR specific for MmmSC. All gave negative results. The detection level of cluster-specific primers and the more specific primers was 33.4 pg of DNA, whereas that of nested PCR was 0.33 pg.<\/jats:p>","DOI":"10.1556\/004.48.2000.4.1","type":"journal-article","created":{"date-parts":[[2015,12,4]],"date-time":"2015-12-04T14:13:10Z","timestamp":1449238390000},"page":"375-385","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Screening of Hungarian Cattle Herds for Mycoplasma mycoides Subspecies Mycoides Small Colony Infection with Negative Results"],"prefix":"10.1556","volume":"48","author":[{"given":"L.","family":"Stipkovits","sequence":"first","affiliation":[{"name":"Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1","family":"D\u00e1n","sequence":"additional","affiliation":[{"name":"Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Varga","sequence":"additional","affiliation":[{"name":"Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"De Santis","sequence":"additional","affiliation":[{"name":"Zooprofilattico Sperimentale dell Abruzzo e del Molise \u2018G. Caporale\u2019,, Teramo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosella","family":"Lelly","sequence":"additional","affiliation":[{"name":"Zooprofilattico Sperimentale dell Abruzzo e del Molise \u2018G. Caporale\u2019,, Teramo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9va","family":"Kaszanyitzky","sequence":"additional","affiliation":[{"name":"Central Veterinary Institute, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ildik\u00f3","family":"Ferenczn\u00e9 Paluska","sequence":"additional","affiliation":[{"name":"Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Tenk","sequence":"additional","affiliation":[{"name":"Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Tekes","sequence":"additional","affiliation":[{"name":"Central Veterinary Institute, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Harrach","sequence":"additional","affiliation":[{"name":"Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","container-title":["Acta Veterinaria Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/48\/4\/article-p375.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/004\/48\/4\/article-p375.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/004\/48\/4\/article-p375.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T20:45:28Z","timestamp":1686170728000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/004\/48\/4\/article-p375.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/004.48.2000.4.1","relation":{},"ISSN":["0236-6290","1588-2705"],"issn-type":[{"value":"0236-6290","type":"print"},{"value":"1588-2705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12]]}}}