{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T20:52:56Z","timestamp":1788209576447,"version":"build-2803163510"},"reference-count":66,"publisher":"Akademiai Kiado Zrt.","issue":"2","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Molecular research of oral diseases"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biofilm formation by oral bacteria on the implant surface is one of the most remarkable factors of peri-implant infections, which may eventually lead to bone resorption and loss of the dental implant. Therefore, the elimination of biofilm is an essential step for the successful therapy of implant-related infections. In this work we created a basic<jats:italic>in vitro<\/jats:italic>model to evaluate the antibacterial effect of three widely used antiseptics.<\/jats:p><jats:p>Commercially pure (CP4) titanium sample discs with sand blasted, acid etched, and polished surface were used. The discs were incubated with mono-cultures of<jats:italic>Streptococcus mitis<\/jats:italic>and<jats:italic>Streptococcus salivarius<\/jats:italic>. The adhered bacterial biofilms were treated with different antiseptics: chlorhexidine-digluconate (CHX), povidone-iodine (PI), and chlorine dioxide (CD) for 5\u2009min and the control discs with ultrapure water. The antibacterial effect of the antiseptics was tested by colorimetric assay.<\/jats:p><jats:p>According to the results, the PI and the CD were statistically the most effective in the elimination of the two test bacteria on both titanium surfaces after 5\u2009min treatment time. The CD showed significant effect only against<jats:italic>S. salivarius<\/jats:italic>.<\/jats:p><jats:p>Based on our results we conclude that PI and CD may be promising antibacterial agents to disinfecting the peri-implant site in the dental practice.<\/jats:p>","DOI":"10.1556\/030.2020.01080","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T07:37:48Z","timestamp":1583998668000},"page":"127-132","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":8,"title":["A simplified in vitro model for investigation of the antimicrobial efficacy of various antiseptic agents to prevent peri-implantitis"],"prefix":"10.1556","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1893-7526","authenticated-orcid":true,"given":"Annam\u00e1ria","family":"Venkei","sequence":"first","affiliation":[{"name":"1Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725, Semmelweis u. 6, Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"E\u00f6rdegh","sequence":"additional","affiliation":[{"name":"2Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, 6720, Tisza Lajos krt. 64, Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinga","family":"Turz\u00f3","sequence":"additional","affiliation":[{"name":"3Dentistry Program, Medical School, University of P\u00e9cs, 7621, Dischka Gy. u. 5, P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edit","family":"Urb\u00e1n","sequence":"additional","affiliation":[{"name":"1Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725, Semmelweis u. 6, Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisztina","family":"Ungv\u00e1ri","sequence":"additional","affiliation":[{"name":"2Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, 6720, Tisza Lajos krt. 64, Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref191","first-page":"140","article-title":"Surgical treatmnet of peri-implantitis","volume":"19","author":"Schou","year":"2004","journal-title":"Int J Oral Maxillofac Implants"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1128\/JB.00345-12","article-title":"Growing unculturable bacteria","volume":"194","author":"Stewart","year":"2012","journal-title":"J Bacteriology"},{"key":"ref471","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1128\/JB.00345-12","article-title":"Growing unculturable 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(Roma)"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"435","DOI":"10.4161\/viru.2.5.16140","article-title":"Bacterial interactions in dental biofilm","volume":"2","author":"Huang","year":"2011","journal-title":"Virulence"},{"key":"ref241","doi-asserted-by":"crossref","DOI":"10.1186\/1745-6215-11-14","article-title":"Effects of a mouthwash with chlorine dioxide on oral malodor and salivary bacteria: a randomized placebo-controlled 7-day trial","volume":"11","author":"Shinada","year":"2010","journal-title":"Trials"},{"key":"ref631","first-page":"153","article-title":"The efficacy of povidone-iodine oral rinse in preventing ventilator-associated pneumonia: a randomized, double blind, placebo-controlled (VAPOR) trial: preliminary report","volume":"33","author":"Chua","year":"2004","journal-title":"Philipp J Microbiol Infect Dis"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3184\/003685001783238998","article-title":"Bacterial biofilms and human 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Biomater"},{"key":"ref01","doi-asserted-by":"crossref","DOI":"10.1034\/j.1600-0501.1998.090202.x","article-title":"Soft tissue response to plaque formation at different implant systems. A comparative study in the dog","volume":"9","author":"Abrahamsson","year":"1998","journal-title":"Clin Oral Implants Res"},{"key":"ref151","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the normal bacterial flora of the oral cavity","volume":"43","author":"Aas","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref271","first-page":"489","article-title":"Toxin effects from chlorhexidine gluconate: case report","volume":"94","author":"Kenrad","year":"1990","journal-title":"Tandlaegebladet"},{"key":"ref181","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0172095","article-title":"Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials","volume":"12","author":"Rath","year":"2017","journal-title":"PLoS One"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1600-051X.2008.01274.x","article-title":"Definition and prevalence of peri-implant diseases","volume":"35","author":"Zitzmann","year":"2008","journal-title":"J Clin Periodontol"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/sj.bdj.4805707","article-title":"Extrinsic tooth discoloration by metals and chlorhexidine. 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Surface protein denaturation or dietary precipitation?","volume":"159","author":"Addy","year":"1985","journal-title":"Br Dent J"},{"key":"ref301","first-page":"153","article-title":"The efficacy of povidone-iodine oral rinse in preventing ventilator-associated pneumonia: a randomized, double blind, placebo-controlled (VAPOR) trial: preliminary report","volume":"33","author":"Chua","year":"2004","journal-title":"Philipp J Microbiol Infect Dis"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1742-481X.2007.00405.x","article-title":"Povidone-iodine: use in hand disinfection, skin preparation and antiseptic irrigation","volume":"5","author":"Durani","year":"2008","journal-title":"Int Wound J"},{"key":"ref621","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/0305-4179(88)90074-5","article-title":"Induction of hyperthyroidism in burn patients treated topically with povidone-iodine","volume":"14","author":"Rath","year":"1988","journal-title":"Burns Incl Therm Inj"},{"key":"ref541","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4103\/0972-124X.107466","article-title":"Biofilm and dental implant: the microbial link","volume":"17","author":"Dhir","year":"2013","journal-title":"J Indian Soc Periodontology"},{"key":"ref641","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1111\/ijcp.12707","article-title":"Practical use of povidone-iodine antiseptic in the maintenance of oral health and in the prevention and treatment of common oropharyngeal infections","volume":"69","author":"Kanagalingam","year":"2015","journal-title":"Int J Clin Pract"},{"key":"ref321","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0079157","article-title":"Chlorine dioxide is a size-selective antimicrobial agent","volume":"8","author":"Noszticzius","year":"2013","journal-title":"PLoS One"},{"key":"ref521","first-page":"140","article-title":"Surgical treatmnet of peri-implantitis","volume":"19","author":"Schou","year":"2004","journal-title":"Int J Oral Maxillofac Implants"},{"key":"ref281","first-page":"35","article-title":"A comparative, randomized, controlled study on clinical efficacy and dental staining reduction of a mouthwash containing chlorhexidine 0.20% and Anti Discoloration System (ADS)","volume":"6","author":"Marrelli","year":"2015","journal-title":"Ann Stomatol (Roma)"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1155\/2014\/487903","article-title":"Treatment alternatives to negotiate peri-implantitis","volume":"2014","author":"Machtei","year":"2014","journal-title":"Advances Med"},{"key":"ref251","first-page":"45","article-title":"Chlorhexidine: the gold standard in chemical plaque control","volume":"1","author":"Mathur","year":"2011","journal-title":"Natl J Physiol Pharm Pharmacol"},{"key":"ref331","doi-asserted-by":"crossref","DOI":"10.1034\/j.1600-0501.1998.090202.x","article-title":"Soft tissue response to plaque formation at different implant systems. A comparative study in the dog","volume":"9","author":"Abrahamsson","year":"1998","journal-title":"Clin Oral Implants Res"},{"key":"ref111","first-page":"67","article-title":"Evidence for the microbicidal activity of a chlorine dioxide-containing oral rinse formulation in vivo","volume":"12","author":"Grootveld","year":"2001","journal-title":"J Clin Dent"},{"key":"ref601","first-page":"489","article-title":"Toxin effects from chlorhexidine gluconate: case report","volume":"94","author":"Kenrad","year":"1990","journal-title":"Tandlaegebladet"},{"key":"ref411","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/jbm.b.31644","article-title":"Effects on titanium implant surfaces of chemical agents used for the treatment of peri-implantitis","volume":"94","author":"Ungvari","year":"2010","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.archoralbio.2011.09.005","article-title":"Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum","volume":"57","author":"Hosaka","year":"2012","journal-title":"Arch Oral Biol"},{"key":"ref511","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0172095","article-title":"Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials","volume":"12","author":"Rath","year":"2017","journal-title":"PLoS One"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1742-481X.2007.00405.x","article-title":"Povidone-iodine: use in hand disinfection, skin preparation and antiseptic irrigation","volume":"5","author":"Durani","year":"2008","journal-title":"Int Wound J"},{"key":"ref591","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/sj.bdj.4805707","article-title":"Extrinsic tooth discoloration by metals and chlorhexidine. 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Surface protein denaturation or dietary precipitation?","volume":"159","author":"Addy","year":"1985","journal-title":"Br Dent J"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1023\/A:1018593403126","article-title":"Failure patterns of four osseointegrated oral implant systems","volume":"8","author":"Esposito","year":"1997","journal-title":"J Mater Sci Mater Med"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"4457","DOI":"10.1016\/j.biomaterials.2003.11.031","article-title":"Evaluation of bacterial adhesion of Streptococcus mutans on dental restorative materials","volume":"24","author":"Montanaro","year":"2004","journal-title":"Biomaterials"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1126\/science.3629258","article-title":"Biomaterial-centered infection: microbial adhesion versus tissue integration","volume":"237","author":"Gristina","year":"1987","journal-title":"Science"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1600-051X.2008.01274.x","article-title":"Definition and prevalence of peri-implant diseases","volume":"35","author":"Zitzmann","year":"2008","journal-title":"J Clin Periodontol"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/0305-4179(88)90074-5","article-title":"Induction of hyperthyroidism in burn patients treated topically with povidone-iodine","volume":"14","author":"Rath","year":"1988","journal-title":"Burns Incl Therm Inj"}],"container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/030.2020.01080","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/67\/2\/article-p127.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/67\/2\/article-p127.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:52:03Z","timestamp":1722559923000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/67\/2\/article-p127.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":66,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/030.2020.01080","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6]]}}}