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In this study, phenotypic and genotypic methods were used to characterize carbapenemase-production and other resistance-determinants (AmpC and ESBL-production, efflux pump-overexpression) in 50 isolates (<jats:italic>Klebsiella<\/jats:italic> spp. <jats:italic>n<\/jats:italic> = 35, <jats:italic>Escherichia coli n<\/jats:italic> = 12 and <jats:italic>Enterobacter cloacae<\/jats:italic> complex <jats:italic>n<\/jats:italic> = 3) collected at the Albert Szent-Gy\u00f6rgyi Clinical Center (University of Szeged) between 2014 and 2017. Minimum inhibitory concentrations of meropenem, sulfamethoxazole\/trimethoprim, tigecycline, amikacin, moxifloxacin, colistin and fosfomycin were also determined. 24% of isolates were AmpC-producers, while 30% carried <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> ESBL-genes. Carbapenemase-genes were detected in 18 (36%) of the tested isolates: in 2 isolates <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>NDM<\/jats:italic><\/jats:sub>, in 6 isolates <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>OXA-48-like<\/jats:italic><\/jats:sub> and in 12 isolates, <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>VIM<\/jats:italic><\/jats:sub> was detected by PCR. The species-distribution for isolates positive for carbapenemase-genes was the following: <jats:italic>Klebsiella pneumoniae n<\/jats:italic> = 11, <jats:italic>Klebsiella oxytoca n<\/jats:italic> = 1, <jats:italic>E. coli n<\/jats:italic> = 5, <jats:italic>E. cloacae<\/jats:italic> complex <jats:italic>n<\/jats:italic> = 1. Efflux pump-overexpression based on the PA\u03b2N-screening agar was shown in <jats:italic>n<\/jats:italic> = 3 of the tested strains. In nine isolates (18%), carbapenemase and ESBL-genes were detected simultaneously. Highest levels of resistance were noted for fosfomycin (74%) and moxifloxacin (70%), while all isolates were susceptible to colistin. Among applied phenotypic tests in this study the modified carbapenem inactivation method (mCIM) proved to be the most accurate one compared to that of PCR results.<\/jats:p>","DOI":"10.1556\/030.2020.01181","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T03:07:07Z","timestamp":1607137627000},"page":"209-215","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":24,"title":["Detection of VIM, NDM and OXA-48 producing carbapenem resistant Enterobacterales among clinical isolates in Southern Hungary"],"prefix":"10.1556","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1270-0365","authenticated-orcid":true,"given":"M\u00e1ri\u00f3","family":"Gajd\u00e1cs","sequence":"first","affiliation":[{"name":"1Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s utca 6., 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"\u00c1br\u00f3k","sequence":"additional","affiliation":[{"name":"2Institute of Clinical Microbiology, Albert Szent-Gy\u00f6rgyi Clinical Center, University of Szeged, Semmelweis utca 6., 6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"L\u00e1z\u00e1r","sequence":"additional","affiliation":[{"name":"2Institute of Clinical Microbiology, Albert Szent-Gy\u00f6rgyi Clinical Center, University of Szeged, Semmelweis utca 6., 6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"J\u00e1nv\u00e1ri","sequence":"additional","affiliation":[{"name":"3Department of Bacteriology, Mycology and Parasitology, National Public Health Centre, Albert Fl\u00f3ri\u00e1n \u00fat 2-6., 1097 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1kos","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"3Department of Bacteriology, Mycology and Parasitology, National Public Health Centre, Albert Fl\u00f3ri\u00e1n \u00fat 2-6., 1097 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Terhes","sequence":"additional","affiliation":[{"name":"3Department of Bacteriology, Mycology and Parasitology, National Public Health Centre, Albert Fl\u00f3ri\u00e1n \u00fat 2-6., 1097 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Buri\u00e1n","sequence":"additional","affiliation":[{"name":"2Institute of Clinical Microbiology, Albert Szent-Gy\u00f6rgyi Clinical Center, University of Szeged, Semmelweis utca 6., 6725 Szeged, Hungary"},{"name":"4Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, D\u00f3m 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