{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T10:47:39Z","timestamp":1770893259704,"version":"3.50.1"},"reference-count":46,"publisher":"Akademiai Kiado Zrt.","issue":"2","funder":[{"name":"Health Sciences University BAP","award":["2019\/021"],"award-info":[{"award-number":["2019\/021"]}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2021,6,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study investigation of plasmid-mediated <jats:italic>mcr 1-5<\/jats:italic> resistance genes was performed among multidrug-resistant (MDR) colistin sensitive and resistant <jats:italic>Klebsiella pneumoniae<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> strains isolated in our laboratory. We aimed to evaluate automated system (Vitek-2), broth microdilution (BMD) reference method and chromogenic media performance. Totally 94 MDR <jats:italic>K. pneumoniae<\/jats:italic> and six <jats:italic>E. coli<\/jats:italic> isolates were included in the study. CHROMID\u00ae Colistin R agar (COLR) (bioMerieux, France) was used to determine the colistin resistance by chromogenic method. Standard PCR amplification was performed using specific primers to screen the plasmid-mediated <jats:italic>mcr 1-5<\/jats:italic> genes. Sixty-one isolates were resistant to colistin and 39 were susceptible with reference BMD. The essential and categorical agreement of Vitek-2 was determined as 100 and 99%. The sensitivity of COLR medium was 100%, the specificity was 97.5%. In our study <jats:italic>mcr-1<\/jats:italic> was detected in eight isolates, while other <jats:italic>mcr<\/jats:italic> genes were not detected. Due to the high sensitivity and specificity of the COLR medium, it can be used in routine diagnostics for the detection of colistin resistance. In our study we detected 8% prevalence of <jats:italic>mcr-1<\/jats:italic> among MDR strains however, two <jats:italic>mcr-1<\/jats:italic> positive isolates were found sensitive to colistin by BMD.<\/jats:p>","DOI":"10.1556\/030.2021.01328","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T09:25:00Z","timestamp":1623662700000},"page":"99-106","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":5,"title":["Detection of colistin resistance among multidrug-resistant Klebsiella pneumoniae and Escherichia coli clinical isolates in Turkey"],"prefix":"10.1556","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1117-3906","authenticated-orcid":true,"given":"Nilg\u00fcn","family":"Kansak","sequence":"first","affiliation":[{"name":"1Medical Microbiology Laboratory, Haydarpa\u015fa Numune Training and Research Hospital, University of Health Sciences, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebahat","family":"Aksaray","sequence":"additional","affiliation":[{"name":"2Department of Medical Microbiology, University of Health Sciences-Hamidiye Medical Faculty, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00fcge","family":"Aslan","sequence":"additional","affiliation":[{"name":"1Medical Microbiology Laboratory, Haydarpa\u015fa Numune Training and Research Hospital, University of Health Sciences, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u0131za","family":"Adaleti","sequence":"additional","affiliation":[{"name":"1Medical Microbiology Laboratory, Haydarpa\u015fa Numune Training and Research Hospital, University of Health Sciences, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nevriye","family":"G\u00f6n\u00fcll\u00fc","sequence":"additional","affiliation":[{"name":"3Department of Medical 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