{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:52:51Z","timestamp":1787169171529,"version":"build-2736575974"},"reference-count":110,"publisher":"Akademiai Kiado Zrt.","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2021,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Infection of the urinary tract ranks as one of the most common infections affecting people worldwide and its treatment is made complicated by the rising incidence of antibiotic resistance. This study aimed to detect extended spectrum beta-lactamase (ESBL) genes and antibiotic resistance profile of uropathogenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ) recovered from patients attending a University Teaching hospital in Nigeria. Uropathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates were obtained from the culture collection of Department of Microbiology and Parasitology of the University Teaching hospital for a period of four months (October 2019\u2013January, 2020). Antibiotic susceptibility testing was done using the disc diffusion method while phenotypic ESBL production was detected using double disc synergy test (DDST). Detection of \u03b2-lactamase genes was done using Real-Time PCR. Forty-nine\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates were recovered from 120 urine samples, with 24 (49%) being ESBL positive. The resistance to antibiotics in the ESBL producers was: ciprofloxacin (100%), cefotaxime (100%), cefpodoxime (100%), tetracycline (95.7%), ceftazidime (56.7%), amoxicillin-clavulanate (50%), gentamicin (33.3%), and imipenem (0%). All the ESBL producers carried\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-1<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-9<\/jats:sub>\n                    , 75% (18\/24) carried\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV<\/jats:sub>\n                    , while\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-2<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-8<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-25<\/jats:sub>\n                    groups were detected in 20.8% (5\/24) of the isolates. There was co-occurrence of CTX-M, SHV and TEM \u03b2-lactamases in 79.2% (19\/24) isolates, while five isolates (20.8%) co-harbored\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    . This study showed a high level of multidrug resistance and ESBL gene carriage in uropathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    obtained in this study, suggesting a likely review of therapeutic options in the treatment of UTI to clamp down on the rising cases of antibiotic resistance.\n                  <\/jats:p>","DOI":"10.1556\/030.2021.01380","type":"journal-article","created":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T11:47:32Z","timestamp":1620388052000},"update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":3,"title":["Molecular characterization of ESBL- producing uropathogenic Escherichia coli recovered from urine samples of patients attending a University Teaching hospital in Nigeria"],"prefix":"10.1556","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2871-1002","authenticated-orcid":true,"given":"Abimbola Olumide","family":"Adekanmbi","sequence":"first","affiliation":[{"name":"1Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3604-1010","authenticated-orcid":true,"given":"Sandra","family":"Usidamen","sequence":"additional","affiliation":[{"name":"2Pathogenic Microbiology Laboratory, Department of Microbiology, University of Ibadan, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Abiodun","family":"Onilude","sequence":"additional","affiliation":[{"name":"3Microbial Physiology and Biochemistry Laboratory, Department of Microbiology, University of Ibadan, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref571","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3126\/kumj.v9i4.6348","article-title":"Microbiological analysis of isolates in 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