{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T05:09:36Z","timestamp":1747199376346,"version":"3.40.5"},"reference-count":70,"publisher":"Akademiai Kiado Zrt.","funder":[{"name":"Ministry of Education, Science and Technological Development, Republic of Serbia","award":["451-03-9\/2021-14\/200015"],"award-info":[{"award-number":["451-03-9\/2021-14\/200015"]}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2021,7,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present study, the effectiveness of six antimicrobial agents have been tested against 24 borrelia strains isolated from <jats:italic>Ixodes ricinus<\/jats:italic> ticks (11 <jats:italic>Borrelia lusitaniae<\/jats:italic>, eight <jats:italic>Borrelia afzelii<\/jats:italic>, three <jats:italic>Borrelia garinii<\/jats:italic> and two <jats:italic>Borrelia valaisiana<\/jats:italic>) and one <jats:italic>B. lusitaniae<\/jats:italic> strain isolated from human skin. The minimum inhibitory concentration range of antimicrobial agents was as follows: amoxicillin, 0.125\u20132 mg\/L; doxycycline, 0.125\u20131 mg\/L, ceftriaxone, 0.016\u20130.063 mg\/L; cefuroxime, 0.063\u20131 mg\/L; azithromycin, 0.0017\u20130.11 mg\/L; amikacin 32\u2013512 mg\/L. Potentially pathogenic <jats:italic>B. lusitaniae<\/jats:italic> and <jats:italic>B. valaisiana<\/jats:italic> species were more susceptible to amoxicillin and azithromycin than pathogenic <jats:italic>B. afzelii<\/jats:italic> and <jats:italic>B. garinii<\/jats:italic> (<jats:italic>P<\/jats:italic> &lt; 0.05); <jats:italic>B. garinii<\/jats:italic>, <jats:italic>B. lusitaniae<\/jats:italic> and <jats:italic>B. valaisiana<\/jats:italic> were more susceptible to doxycycline than <jats:italic>B. afzelii<\/jats:italic> (<jats:italic>P<\/jats:italic> &lt; 0.05) while all species showed same susceptibility to ceftriaxone and cefuroxime (<jats:italic>P<\/jats:italic> &gt; 0.05). This study is the first report on <jats:italic>in vitro<\/jats:italic> susceptibility of isolates from Serbia to antimicrobial agents and the first report on susceptibility of larger number of isolates of potentially pathogenic species <jats:italic>B. lusitaniae<\/jats:italic>. We showed that antimicrobial agents <jats:italic>in vitro<\/jats:italic> inhibit growth of borrelia strains very effectively, indicating the potential of their equally beneficial use in the treatment of Lyme borreliosis.<\/jats:p>","DOI":"10.1556\/030.2021.01441","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T23:46:07Z","timestamp":1625615167000},"update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":0,"title":["In vitro efficacy of antibiotics against different Borrelia isolates"],"prefix":"10.1556","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8606-319X","authenticated-orcid":true,"given":"Gorana","family":"Veinovi\u0107","sequence":"first","affiliation":[{"name":"1Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanja","family":"\u0106aki\u0107","sequence":"additional","affiliation":[{"name":"1Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darko","family":"Mihaljica","sequence":"additional","affiliation":[{"name":"1Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ratko","family":"Sukara","sequence":"additional","affiliation":[{"name":"1Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Ru\u017ei\u0107\u2013Sablji\u0107","sequence":"additional","affiliation":[{"name":"2Laboratory for Diagnostics of Borreliosis and Leptospirosis, Institute for Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sne\u017eana","family":"Tomanovi\u0107","sequence":"additional","affiliation":[{"name":"1Group for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"ref671","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ttbdis.2009.11.004","article-title":"Comparison of in vitro activities of tigecycline, doxycycline, and tetracycline against the spirochete Borrelia 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