{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:41:26Z","timestamp":1783557686738,"version":"3.55.0"},"reference-count":88,"publisher":"Akademiai Kiado Zrt.","funder":[{"name":"European Union's Horizon 2020","award":["874735"],"award-info":[{"award-number":["874735"]}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2021,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) constitutes a constant threat for the public health. Aim of the present study was to analyse the whole genome sequences of two MRSA strains belonging to <jats:italic>Staphylococcus<\/jats:italic> protein A <jats:italic>(spa)<\/jats:italic> type t127 isolated from humans working in two distantly located dairy production farms in Greece.<\/jats:p><jats:p>MRSA strains were isolated from the nasal cavity of a food handler in a milk industry in Epirus, northwestern Greece (E-MRSA), and a person working in a cattle farm in Thrace, northeastern Greece (T-MRSA). Whole genome sequences taken using next generation sequencing were analysed for resistance and virulence genes applying various bioinformatic tools.<\/jats:p><jats:p>Both isolates were assigned to ST1-IVa-t127 type, and they were transferring genes conferring resistance to tetracycline, \u03b2-lactams, and aminoglycosides; T-MRSA was carrying additional genes leading to macrolide, lincosamide and streptogramin B (MLS<jats:sub>B<\/jats:sub>) resistance. Both isolates were carrying three plasmid replicon types, rep5, rep7 and rep16, while T-MRSA harboured also rep10 and rep15. E-MRSA carried <jats:italic>scn<\/jats:italic> and <jats:italic>sak<\/jats:italic> genes which were absent from T-MRSA.<\/jats:p><jats:p>In conclusion, the genetic characterization of two unrelated ST1-IVa-t127 MRSA strains isolated from humans in close contact with livestock in Greece can be used as basis for further epidemiological and evolutionary studies.<\/jats:p>","DOI":"10.1556\/030.2021.01460","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T09:11:02Z","timestamp":1623834662000},"update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":3,"title":["Genetic characterization of two methicillin-resistant Staphylococcus aureus spa type t127 strains isolated from workers in the dairy production chain in Greece"],"prefix":"10.1556","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0928-3193","authenticated-orcid":true,"given":"Theodoros","family":"Karampatakis","sequence":"first","affiliation":[{"name":"1Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"2Laboratory of Food Hygiene-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Tsergouli","sequence":"additional","affiliation":[{"name":"1Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apostolos S.","family":"Angelidis","sequence":"additional","affiliation":[{"name":"3Laboratory of Safety and Quality of Milk and Dairy Products, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Sergelidis","sequence":"additional","affiliation":[{"name":"2Laboratory of Food Hygiene-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Papa","sequence":"additional","affiliation":[{"name":"1Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref221","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia Coli","volume":"52","author":"Joensen","year":"2014","journal-title":"J Clin Microbiol"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1590\/0074-02760170128","article-title":"Complete genome sequence of community-associated methicillin-resistant Staphylococcus aureus (strain USA400-0051), a prototype of the USA400 clone","volume":"112","author":"Cortes","year":"2017","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref781","doi-asserted-by":"crossref","first-page":"a027037","DOI":"10.1101\/cshperspect.a027037","article-title":"Lincosamides, streptogramins, phenicols, and pleuromutilins: Mode of action and mechanisms of resistance","volume":"6","author":"Schwarz","year":"2016","journal-title":"Cold Spring Harb Perspect Med"},{"key":"ref611","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.fm.2017.07.016","article-title":"Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece","volume":"69","author":"Papadopoulos","year":"2018","journal-title":"Food Microbiol"},{"key":"ref791","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1590\/0074-02760170128","article-title":"Complete genome sequence of community-associated methicillin-resistant Staphylococcus aureus (strain USA400-0051), a prototype of the USA400 clone","volume":"112","author":"Cortes","year":"2017","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref121","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/JCM.38.3.1008-1015.2000","article-title":"Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus","volume":"38","author":"Enright","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1469-0691.2010.03227.x","article-title":"Immune evasion cluster-positive bacteriophages are highly prevalent among human Staphylococcus aureus strains, but they are not essential in the first stages of nasal colonization","volume":"17","author":"Verkaik","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"ref771","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.tvjl.2013.06.004","article-title":"Methicillin resistant Staphylococcus aureus (MRSA) carriage in different free-living wild animal species in Spain","volume":"198","author":"Porrero","year":"2013","journal-title":"Vet J"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1128\/JCM.00420-06","article-title":"Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing","volume":"44","author":"Strommenger","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref691","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1186\/1471-2334-13-258","article-title":"Livestock-associated methicillin-resistant Staphylococcus aureus responsible for human colonization and infection in an area of Italy with high density of pig farming","volume":"13","author":"Monaco","year":"2013","journal-title":"BMC Infect Dis"},{"key":"ref421","doi-asserted-by":"crossref","first-page":"e79645","DOI":"10.1371\/journal.pone.0079645","article-title":"Rapid differentiation between livestock-associated and livestock-independent Staphylococcus aureus CC398 clades","volume":"8","author":"Stegger","year":"2013","journal-title":"PLoS One"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"e0137143","DOI":"10.1371\/journal.pone.0137143","article-title":"Livestock-associated methicillin resistant and methicillin susceptible Staphylococcus aureus sequence type (CC) 1 in European farmed animals: high genetic relatedness of isolates from Italian cattle herds and humans","volume":"10","author":"Alba","year":"2015","journal-title":"PLoS One"},{"key":"ref491","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/cid\/cix504","article-title":"Emergence of livestock-associated methicillin-resistant Staphylococcus aureus bloodstream infections in Denmark","volume":"65","author":"Larsen","year":"2017","journal-title":"Clin Infect Dis"},{"key":"ref621","doi-asserted-by":"crossref","first-page":"103249","DOI":"10.1016\/j.fm.2019.103249","article-title":"Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: prevalence, characterization and genetic relatedness","volume":"84","author":"Papadopoulos","year":"2019","journal-title":"Food Microbiol"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/jac\/dki266","article-title":"Surveillance and epidemiology of mrsa bacteraemia in the UK","volume":"56","author":"Johnson","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/jac\/dkr115","article-title":"Molecular characterization of spa type t127, sequence type 1 methicillin-resistant Staphylococcus aureus from pigs","volume":"66","author":"Franco","year":"2011","journal-title":"J Antimicrob Chemother"},{"key":"ref631","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1093\/jac\/dkaa345","article-title":"Resfinder 4.0 for predictions of phenotypes from genotypes","volume":"75","author":"Bortolaia","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"ref701","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/jac\/dkr115","article-title":"Molecular characterization of spa type t127, sequence type 1 methicillin-resistant Staphylococcus aureus from pigs","volume":"66","author":"Franco","year":"2011","journal-title":"J Antimicrob Chemother"},{"key":"ref321","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.vetmic.2013.11.022","article-title":"Identification and characterization of methicillin-resistant Staphylococcus aureus (MRSA) from Austrian companion animals and horses","volume":"168","author":"Loncaric","year":"2014","journal-title":"Vet Microbiol"},{"key":"ref531","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3389\/fmicb.2018.00163","article-title":"Distribution of the most prevalent spa types among clinical isolates of methicillin-resistant and-susceptible Staphylococcus aureus around the world: a review","volume":"9","author":"Asadollahi","year":"2018","journal-title":"Front Microbiol"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.meegid.2019.01.021","article-title":"A novel multidrug-resistant PVL-negative CC1-MRSA-IV clone emerging in Ireland and Germany likely originated in south-eastern Europe","volume":"69","author":"Earls","year":"2019","journal-title":"Infect Genet Evol"},{"key":"ref751","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.meegid.2019.01.021","article-title":"A novel multidrug-resistant PVL-negative CC1-MRSA-IV clone emerging in Ireland and Germany likely originated in south-eastern Europe","volume":"69","author":"Earls","year":"2019","journal-title":"Infect Genet Evol"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.fm.2017.07.016","article-title":"Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece","volume":"69","author":"Papadopoulos","year":"2018","journal-title":"Food Microbiol"},{"key":"ref581","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1089\/mdr.2018.0259","article-title":"MLSb-resistant Staphylococcus aureus in central Greece: Rate of resistance and molecular characterization","volume":"25","author":"Sarrou","year":"2019","journal-title":"Microb Drug Resist"},{"key":"ref151","doi-asserted-by":"crossref","first-page":"e00226","DOI":"10.1128\/mSphere.00226-20","article-title":"Evolution and population dynamics of clonal complex 152 community-associated methicillin-resistant Staphylococcus aureus","volume":"5","author":"Baig","year":"2020","journal-title":"mSphere"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"30236","DOI":"10.2807\/1560-7917.ES.2016.21.21.30236","article-title":"Changing characteristics of livestock-associated meticillin-resistant Staphylococcus aureus isolated from humans \u2013 emergence of a subclade transmitted without livestock exposure, The Netherlands, 2003 to 2014","volume":"21","author":"Bosch","year":"2016","journal-title":"Euro Surveill"},{"key":"ref711","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1089\/mdr.2008.0845","article-title":"Clusters of infections in horses with MRSA ST1, ST254, and ST398 in a veterinary hospital","volume":"14","author":"Cuny","year":"2008","journal-title":"Microb Drug Resist"},{"key":"ref391","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s10295-016-1739-5","article-title":"A novel chimeric prophage vB_LdeS-phiJB from commercial Lactobacillus delbrueckii subsp. bulgaricus","volume":"43","author":"Guo","year":"2016","journal-title":"J Ind Microbiol Biotechnol"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1128\/AAC.46.4.1147-1152.2002","article-title":"Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains","volume":"46","author":"Ma","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"key":"ref431","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.vetmic.2015.02.031","article-title":"Prevalence of the immune evasion gene cluster in Staphylococcus aureus CC398","volume":"177","author":"Cuny","year":"2015","journal-title":"Vet Microbiol"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.scitotenv.2019.03.110","article-title":"Genomic analysis of methicillin-resistant Staphylococcus aureus isolated from poultry and occupational farm workers in umgungundlovu district, South Africa","volume":"670","author":"Amoako","year":"2019","journal-title":"Sci Total Environ"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3389\/fmicb.2018.00163","article-title":"Distribution of the most prevalent spa types among clinical isolates of methicillin-resistant and-susceptible Staphylococcus aureus around the world: a review","volume":"9","author":"Asadollahi","year":"2018","journal-title":"Front Microbiol"},{"key":"ref361","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1128\/JCM.00657-10","article-title":"Virulence genes and genotypic associations in nasal carriage, community-associated methicillin-susceptible and methicillin-resistant USA400 Staphylococcus aureus isolates","volume":"48","author":"Shukla","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref471","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.3201\/eid1112.050428","article-title":"Methicillin-resistant Staphylococcus aureus in pig farming","volume":"11","author":"Voss","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"103249","DOI":"10.1016\/j.fm.2019.103249","article-title":"Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in bulk tank milk, livestock and dairy-farm personnel in north-central and north-eastern Greece: prevalence, characterization and genetic relatedness","volume":"84","author":"Papadopoulos","year":"2019","journal-title":"Food Microbiol"},{"key":"ref811","doi-asserted-by":"crossref","first-page":"e0137143","DOI":"10.1371\/journal.pone.0137143","article-title":"Livestock-associated methicillin resistant and methicillin susceptible Staphylococcus aureus sequence type (CC) 1 in European farmed animals: high genetic relatedness of isolates from Italian cattle herds and humans","volume":"10","author":"Alba","year":"2015","journal-title":"PLoS One"},{"key":"ref111","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00612-17","article-title":"SCCmecFinder, a web-based tool for typing of staphylococcal cassette chromosome mec in Staphylococcus aureus using whole-genome sequence data","volume":"3","author":"Kaya","year":"2018","journal-title":"mSphere"},{"key":"ref511","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1186\/s12879-017-2802-1","article-title":"Longitudinal study of Staphylococcus aureus colonization and infection in a cohort of swine veterinarians in the united states","volume":"17","author":"Sun","year":"2017","journal-title":"BMC Infect Dis"},{"key":"ref641","first-page":"517","article-title":"Antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic Acids Res"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/nar\/gkw387","article-title":"Phaster: a better, faster version of the PHAST PHAge search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"ref271","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1089\/mdr.2008.0845","article-title":"Clusters of infections in horses with MRSA ST1, ST254, and ST398 in a veterinary hospital","volume":"14","author":"Cuny","year":"2008","journal-title":"Microb Drug Resist"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1097\/JOM.0000000000001385","article-title":"Prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) among farm and slaughterhouse workers in Italy","volume":"60","author":"Mascaro","year":"2018","journal-title":"J Occup Environ Med"},{"key":"ref841","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1111\/j.1469-0691.2010.03227.x","article-title":"Immune evasion cluster-positive bacteriophages are highly prevalent among human Staphylococcus aureus strains, but they are not essential in the first stages of nasal colonization","volume":"17","author":"Verkaik","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"ref731","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1128\/AAC.46.4.1147-1152.2002","article-title":"Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains","volume":"46","author":"Ma","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"key":"ref481","doi-asserted-by":"crossref","first-page":"3817","DOI":"10.1128\/AAC.00613-08","article-title":"Antimicrobial resistance of old and recent Staphylococcus aureus isolates from poultry: first detection of livestock-associated methicillin-resistant strain ST398","volume":"52","author":"Nemati","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jhin.2019.01.025","article-title":"Contribution of whole-genome sequencing to understanding of the epidemiology and control of meticillin-resistant Staphylococcus aureus","volume":"102","author":"Humphreys","year":"2019","journal-title":"J Hosp Infect"},{"key":"ref241","doi-asserted-by":"crossref","first-page":"e1000215","DOI":"10.1371\/journal.pmed.1000215","article-title":"Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: a molecular-epidemiological analysis","volume":"7","author":"Grundmann","year":"2010","journal-title":"PLoS Med"},{"key":"ref601","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.3389\/fmicb.2018.01436","article-title":"Molecular typing of ST239-MRSA-III from diverse geographic locations and the evolution of the SCCmec III element during its intercontinental spread","volume":"9","author":"Monecke","year":"2018","journal-title":"Front Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.3201\/eid1112.050428","article-title":"Methicillin-resistant Staphylococcus aureus in pig farming","volume":"11","author":"Voss","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"ref851","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00305-11","article-title":"Staphylococcus aureus cc398: host adaptation and emergence of methicillin resistance in livestock","volume":"3","author":"Price","year":"2012","journal-title":"mBio"},{"key":"ref331","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.tvjl.2013.06.004","article-title":"Methicillin resistant Staphylococcus aureus (MRSA) carriage in different free-living wild animal species in Spain","volume":"198","author":"Porrero","year":"2013","journal-title":"Vet J"},{"key":"ref831","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s10295-016-1739-5","article-title":"A novel chimeric prophage vB_LdeS-phiJB from commercial Lactobacillus delbrueckii subsp. bulgaricus","volume":"43","author":"Guo","year":"2016","journal-title":"J Ind Microbiol Biotechnol"},{"key":"ref451","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1469-0691.2006.01341.x","article-title":"Community-acquired methicillin-resistant Staphylococcus aureus: current perspectives","volume":"12","author":"Kluytmans-Vandenbergh","year":"2006","journal-title":"Clin Microbiol Infect"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"a027037","DOI":"10.1101\/cshperspect.a027037","article-title":"Lincosamides, streptogramins, phenicols, and pleuromutilins: Mode of action and mechanisms of resistance","volume":"6","author":"Schwarz","year":"2016","journal-title":"Cold Spring Harb Perspect Med"},{"key":"ref871","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.vetmic.2015.02.031","article-title":"Prevalence of the immune evasion gene cluster in Staphylococcus aureus CC398","volume":"177","author":"Cuny","year":"2015","journal-title":"Vet Microbiol"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s10096-008-0698-9","article-title":"Comparison of community-associated methicillin-resistant Staphylococcus aureus from teaching hospitals in London and the USA, 2004\u20132006: where is USA300 in the UK?","volume":"28","author":"Otter","year":"2009","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref571","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jhin.2019.01.025","article-title":"Contribution of whole-genome sequencing to understanding of the epidemiology and control of meticillin-resistant Staphylococcus aureus","volume":"102","author":"Humphreys","year":"2019","journal-title":"J Hosp Infect"},{"key":"ref741","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3390\/antibiotics4040521","article-title":"Livestock-associated MRSA: the impact on humans","volume":"4","author":"Cuny","year":"2015","journal-title":"Antibiotics (Basel)"},{"key":"ref441","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/jac\/dki266","article-title":"Surveillance and epidemiology of mrsa bacteraemia in the UK","volume":"56","author":"Johnson","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"ref541","doi-asserted-by":"crossref","first-page":"30236","DOI":"10.2807\/1560-7917.ES.2016.21.21.30236","article-title":"Changing characteristics of livestock-associated meticillin-resistant Staphylococcus aureus isolated from humans \u2013 emergence of a subclade transmitted without livestock exposure, The Netherlands, 2003 to 2014","volume":"21","author":"Bosch","year":"2016","journal-title":"Euro Surveill"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1093\/jac\/dkaa345","article-title":"Resfinder 4.0 for predictions of phenotypes from genotypes","volume":"75","author":"Bortolaia","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"ref551","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00612-17","article-title":"SCCmecFinder, a web-based tool for typing of staphylococcal cassette chromosome mec in Staphylococcus aureus using whole-genome sequence data","volume":"3","author":"Kaya","year":"2018","journal-title":"mSphere"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/cid\/cix504","article-title":"Emergence of livestock-associated methicillin-resistant Staphylococcus aureus bloodstream infections in Denmark","volume":"65","author":"Larsen","year":"2017","journal-title":"Clin Infect Dis"},{"key":"ref591","doi-asserted-by":"crossref","first-page":"e00226","DOI":"10.1128\/mSphere.00226-20","article-title":"Evolution and population dynamics of clonal complex 152 community-associated methicillin-resistant Staphylococcus aureus","volume":"5","author":"Baig","year":"2020","journal-title":"mSphere"},{"key":"ref411","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00305-11","article-title":"Staphylococcus aureus cc398: host adaptation and emergence of methicillin resistance in livestock","volume":"3","author":"Price","year":"2012","journal-title":"mBio"},{"key":"ref561","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/JCM.38.3.1008-1015.2000","article-title":"Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus","volume":"38","author":"Enright","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1186\/s12879-017-2802-1","article-title":"Longitudinal study of Staphylococcus aureus colonization and infection in a cohort of swine veterinarians in the united states","volume":"17","author":"Sun","year":"2017","journal-title":"BMC Infect Dis"},{"key":"ref661","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia Coli","volume":"52","author":"Joensen","year":"2014","journal-title":"J Clin Microbiol"},{"key":"ref301","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3390\/antibiotics4040521","article-title":"Livestock-associated MRSA: the impact on humans","volume":"4","author":"Cuny","year":"2015","journal-title":"Antibiotics (Basel)"},{"key":"ref861","doi-asserted-by":"crossref","first-page":"e79645","DOI":"10.1371\/journal.pone.0079645","article-title":"Rapid differentiation between livestock-associated and livestock-independent Staphylococcus aureus CC398 clades","volume":"8","author":"Stegger","year":"2013","journal-title":"PLoS One"},{"key":"ref521","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1128\/JCM.00420-06","article-title":"Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing","volume":"44","author":"Strommenger","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref821","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.scitotenv.2019.03.110","article-title":"Genomic analysis of methicillin-resistant Staphylococcus aureus isolated from poultry and occupational farm workers in umgungundlovu district, South Africa","volume":"670","author":"Amoako","year":"2019","journal-title":"Sci Total Environ"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1089\/mdr.2018.0259","article-title":"MLSb-resistant Staphylococcus aureus in central Greece: Rate of resistance and molecular characterization","volume":"25","author":"Sarrou","year":"2019","journal-title":"Microb Drug Resist"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1097\/JOM.0000000000001385","article-title":"Prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) among farm and slaughterhouse workers in Italy","volume":"60","author":"Mascaro","year":"2018","journal-title":"J Occup Environ Med"},{"key":"ref801","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1128\/JCM.00657-10","article-title":"Virulence genes and genotypic associations in nasal carriage, community-associated methicillin-susceptible and methicillin-resistant USA400 Staphylococcus aureus isolates","volume":"48","author":"Shukla","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"3817","DOI":"10.1128\/AAC.00613-08","article-title":"Antimicrobial resistance of old and recent Staphylococcus aureus isolates from poultry: first detection of livestock-associated methicillin-resistant strain ST398","volume":"52","author":"Nemati","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"ref161","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.3389\/fmicb.2018.01436","article-title":"Molecular typing of ST239-MRSA-III from diverse geographic locations and the evolution of the SCCmec III element during its intercontinental spread","volume":"9","author":"Monecke","year":"2018","journal-title":"Front Microbiol"},{"key":"ref681","doi-asserted-by":"crossref","first-page":"e1000215","DOI":"10.1371\/journal.pmed.1000215","article-title":"Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: a molecular-epidemiological analysis","volume":"7","author":"Grundmann","year":"2010","journal-title":"PLoS Med"},{"key":"ref721","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s10096-008-0698-9","article-title":"Comparison of community-associated methicillin-resistant Staphylococcus aureus from teaching hospitals in London and the USA, 2004\u20132006: where is USA300 in the UK?","volume":"28","author":"Otter","year":"2009","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref201","first-page":"517","article-title":"Antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic Acids Res"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1469-0691.2006.01341.x","article-title":"Community-acquired methicillin-resistant Staphylococcus aureus: current perspectives","volume":"12","author":"Kluytmans-Vandenbergh","year":"2006","journal-title":"Clin Microbiol Infect"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1289\/EHP35","article-title":"The prevalence of antibiotic-resistant Staphylococcus aureus nasal carriage among industrial hog operation workers, community residents, and children living in their households: North Carolina, USA","volume":"125","author":"Hatcher","year":"2017","journal-title":"Environ Health Perspect"},{"key":"ref251","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1186\/1471-2334-13-258","article-title":"Livestock-associated methicillin-resistant Staphylococcus aureus responsible for human colonization and infection in an area of Italy with high density of pig farming","volume":"13","author":"Monaco","year":"2013","journal-title":"BMC Infect Dis"},{"key":"ref671","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/nar\/gkw387","article-title":"Phaster: a better, faster version of the PHAST PHAge search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"ref761","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.vetmic.2013.11.022","article-title":"Identification and characterization of methicillin-resistant Staphylococcus aureus (MRSA) from Austrian companion animals and horses","volume":"168","author":"Loncaric","year":"2014","journal-title":"Vet Microbiol"},{"key":"ref501","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1289\/EHP35","article-title":"The prevalence of antibiotic-resistant Staphylococcus aureus nasal carriage among industrial hog operation workers, community residents, and children living in their households: North Carolina, USA","volume":"125","author":"Hatcher","year":"2017","journal-title":"Environ Health Perspect"},{"key":"ref211","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"ref651","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/aop\/article-10.1556-030.2021.01460\/article-10.1556-030.2021.01460.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/aop\/article-10.1556-030.2021.01460\/article-10.1556-030.2021.01460.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T23:47:49Z","timestamp":1651535269000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/aop\/article-10.1556-030.2021.01460\/article-10.1556-030.2021.01460.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,15]]},"references-count":88,"URL":"https:\/\/doi.org\/10.1556\/030.2021.01460","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,15]]}}}