{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:21:40Z","timestamp":1770330100965,"version":"3.49.0"},"reference-count":86,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2023,9,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Irreversible pulpitis is an inflammation of the tooth pulp caused by an opportunity-driven invasion of the pulp space by oral microbiota typically prevalent in the oral cavity. Microbial organisms are extensively recognised to be the fundamental cause of endodontic infections and treatment failures. Previously, bacterial species responsible for these infections were largely recognised using conventional microbial culture techniques, lending credence to the widely held belief that anaerobic Gram-negative bacteria frequently enter the pulp space and trigger endodontic infections. The advent of novel technologies grants the advantage of detecting and studying microbial populations via an amalgamation of the modern \u201cOmics\u201d techniques and meticulous bioinformatics analysis, additionally detecting the metatranscriptome, metaproteome and metabolome along with the metagenome. Amongst these analytical strategies, metagenomic analyses are essentially pragmatic for investigating the oral microbiome. Metagenomics favor not only assessment of microbial composition in diseased conditions, but also contributes to detection of novel, potentially pathogenic species inclusive of non-viable bacteria. The present review describes current knowledge of root canal microbiome, including its composition and functional attributes, the novel strategies available for detection of microbiome as well as challenges associated and provides some crucial pointers for areas of future research.<\/jats:p>","DOI":"10.1556\/030.2023.02082","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T12:15:48Z","timestamp":1690546548000},"page":"177-186","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":3,"title":["Role of oral microbiota in irreversible pulpitis \u2013 Current strategies and future perspectives"],"prefix":"10.1556","volume":"70","author":[{"given":"Preethesh","family":"Shetty","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangalore \u2013 575018, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4777-9942","authenticated-orcid":true,"given":"Shishir","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangalore \u2013 575018, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praveen","family":"Rai","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases & Microbial Genomics, Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Mangalore \u2013 575018, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ballamoole Krishna","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases & Microbial Genomics, Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Mangalore \u2013 575018, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raksha","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangalore \u2013 575018, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"issue":"7","key":"B1","series-title":"Microbes Infect","first-page":"505","article-title":"Beyond microbial community composition: functional activities of the oral microbiome in health and disease","volume":"17","author":"Duran-Pinedo AE","year":"2015","unstructured":"Duran-Pinedo AE, Frias-Lopez J. 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