{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:53:55Z","timestamp":1790009635490,"version":"4.0.1"},"reference-count":52,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2024,7,2]]},"abstract":"Abstract<\/jats:title>The present study aimed to explore the genomic characteristics of eight New Delhi metallo-\u03b2-lactamase-1 (NDM-1)-producing carbapenem-resistant Pseudomonas aeruginosa<\/jats:italic> (CRPA) isolates from a Bulgarian tertiary hospital (2021\u20132023) in comparison to bla<\/jats:italic>NDM-1<\/jats:sub>-positive strains originating from the Balkans. Antimicrobial susceptibility testing, phenotypic assays for carbapenemase activity, PCR screening, whole-genome sequencing (WGS), and phylogenomic analysis were performed. Seven of the CRPA isolates investigated (Minimum inhibitory concentration values of imipenem and meropenem >32\u2009mg L\u22121<\/jats:sup>) were also resistant to piperacillin-tazobactam, ceftazidime, ceftazidime-avibactam, cefepime, ceftolozane-tazobactam, amikacin, tobramycin, ciprofloxacin, and levofloxacin, but were susceptible to colistin (0.5\u20132\u2009mg L\u22121<\/jats:sup>) and cefiderocol (0.25\u20131\u2009mg L\u22121<\/jats:sup>). The P. aeruginosa<\/jats:italic> Pae57 isolate (designated Pae57) remained susceptible to aminoglycosides as well. WGS uncovered the co-existence of bla<\/jats:italic>NDM-1<\/jats:sub> and bla<\/jats:italic>GES-1<\/jats:sub>. The isolates belonged to the ST654 high-risk clone, except for Pae57 (ST611). Alignment against reference sequences revealed the presence of a Tn21<\/jats:italic> transposon harboring ble<\/jats:italic>MBL<\/jats:sub>\u2013bla<\/jats:italic>NDM-1<\/jats:sub>\u2013ISAba125<\/jats:italic>. It was similar to that found in the P. aeruginosa<\/jats:italic> ST654 NDM1_1 strain (GCA_020404785.1) from Serbia. Phylogenomic analysis of our isolates indicated that seven of them (ST654) differed from each other in no more than 44 single-nucleotide polymorphisms (SNPs). Pae57 (ST611) was strikingly different (>21,700 SNPs) compared to all Balkan strains. In conclusion, to our knowledge this is the first report of bla<\/jats:italic>NDM-1<\/jats:sub>-positive P. aeruginosa<\/jats:italic> ST611 isolation, which indicates the transmission dynamics of this determinant between high-risk and potentially high-risk P. aeruginosa<\/jats:italic> clones. Obtained results unveil the dissemination of clonally related NDM-1-producing P. aeruginosa<\/jats:italic> strains in the monitored hospital for approximately a 2-year period.<\/jats:p>","DOI":"10.1556\/030.2024.02309","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T09:27:46Z","timestamp":1718011666000},"page":"99-109","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":4,"title":["Genomic insights into NDM-1-producing Pseudomonas aeruginosa: Current status in a Bulgarian tertiary hospital and on the Balkans"],"prefix":"10.1556","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5197-1849","authenticated-orcid":true,"given":"Tanya","family":"Strateva","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology \u201cCorr. Mem. Prof. Ivan Mitov, MD, DMSc\u201d, Faculty of Medicine, Medical University of Sofia, Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Keuleyan","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Virology, University Hospital Lozenetz, Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Slavil","family":"Peykov","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology \u201cCorr. Mem. Prof. Ivan Mitov, MD, DMSc\u201d, Faculty of Medicine, Medical University of Sofia, Sofia, Bulgaria"},{"name":"Department of Genetics, Faculty of Biology, Sofia University \u201cSt. Kliment Ohridski\u201d, Sofia, Bulgaria"},{"name":"BioInfoTech Laboratory, Sofia Tech Park, Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"issue":"3","key":"B1","series-title":"Clin Microbiol Rev","first-page":"e00181","article-title":"Antimicrobial resistance in ESKAPE pathogens","volume":"33","author":"De Oliveira DMP","year":"2020","unstructured":"De Oliveira DMP, Forde BM, Kidd TJ, Harris PNA, Schembri MA, Beatson SA, et\u00a0al. Antimicrobial resistance in ESKAPE pathogens. 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