{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T02:52:28Z","timestamp":1776653548682,"version":"3.51.2"},"reference-count":25,"publisher":"Akademiai Kiado Zrt.","issue":"3","funder":[{"name":"Wuxi Health Commission Research Project","award":["Z202314"],"award-info":[{"award-number":["Z202314"]}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2024,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Klebsiella pneumoniae<\/jats:italic> is an opportunistic pathogen and it can cause human mucosal lesions through the intestine, leading to bacteremia and abscess formation in liver and spleen. Previous studies have shown that <jats:italic>K. pneumoniae<\/jats:italic> can enter or cross cells through the intestinal epithelium, but the mechanism is unknown. In this study, we treated the intestinal epithelial cell line Caco-2 with KP1195, a clinically isolated strain with high adhesion and invasion of intestinal epithelial cells. The results showed that the\u00a0treatment of <jats:italic>K. pneumoniae<\/jats:italic> could increase the expression of integrin gene and further disrupt the changes of cytoskeleton. Treating Caco-2 with cytoskeletal inhibitor cytorelaxin D can significantly increase the efficiency of <jats:italic>K. pneumoniae<\/jats:italic> invading Caco-2 cells. These data suggest that disruption of the cytoskeleton through integrins may be one of the mechanisms by which <jats:italic>K. pneumoniae<\/jats:italic> increases intracellular invasion. This study provides a theoretical basis for further understanding of the mechanism of <jats:italic>K. pneumoniae<\/jats:italic> entering intestinal epithelial cells.<\/jats:p>","DOI":"10.1556\/030.2024.02326","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T13:11:22Z","timestamp":1722345082000},"page":"237-241","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":2,"title":["Klebsiella pneumoniae increases invasion in\u00a0intestinal epithelial cells by disrupting the\u00a0cytoskeleton"],"prefix":"10.1556","volume":"71","author":[{"given":"Xu","family":"Wang","sequence":"first","affiliation":[{"name":"Suzhou Medical College of Soochow University, Suzhou, People's Republic of China"},{"name":"Department of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi 214005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hong","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis Nursing, Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi 214005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Long","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology Surgery, Affiliated Children's Hospital of Jiangnan University, Wuxi Children's Hospital, Wuxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi 214005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hong","family":"Rui","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi 214005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5540-9020","authenticated-orcid":true,"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi 214005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1211-8546","authenticated-orcid":true,"given":"Ping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Fifth People's Hospital of Suzhou, The Affiliated Infectious Diseases Hospital, Suzhou Medical College of Soochow University, Suzhou, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"B1","series-title":"Front Cell Infect Microbiol","article-title":"Klebsiella pneumoniae biofilms and their role in disease pathogenesis","volume":"12","author":"Guerra MES","year":"2022","unstructured":"Guerra MES, Destro G, Vieira B, Lima AS, Ferraz LFC, Hakansson AP, et\u00a0al. Klebsiella pneumoniae biofilms and their role in disease pathogenesis. Front Cell Infect Microbiol 2022; 12: 877995."},{"issue":"3","key":"B2","series-title":"J Intern Med","first-page":"283","article-title":"Hypervirulent Klebsiella pneumoniae - clinical and molecular perspectives","volume":"287","author":"Choby JE","year":"2020","unstructured":"Choby JE, Howard-Anderson J, Weiss DS. Hypervirulent Klebsiella pneumoniae - clinical and molecular perspectives. J Intern Med 2020; 287(3): 283\u2013300."},{"issue":"6","key":"B3","series-title":"Nat Rev Microbiol","first-page":"344","article-title":"Population genomics of Klebsiella pneumoniae","volume":"18","author":"Wyres KL","year":"2020","unstructured":"Wyres KL, Lam MMC, Holt KE. Population genomics of Klebsiella pneumoniae. Nat Rev Microbiol 2020; 18(6): 344\u2013359."},{"issue":"3","key":"B4","series-title":"Clin Microbiol Rev","first-page":"e00001","article-title":"Hypervirulent Klebsiella pneumoniae","volume":"32","author":"Russo TA","year":"2019","unstructured":"Russo TA, Marr CM. Hypervirulent Klebsiella pneumoniae. Clin Microbiol Rev 2019; 32(3): e00001\u201300019."},{"issue":"2","key":"B5","series-title":"Comp Med","first-page":"183","article-title":"Clinical and pathologic features of Spontaneous Klebsiella pneumoniae infection in 9 Rhesus Macaques (Macaca mulatta)","volume":"70","author":"Keesler RI","year":"2020","unstructured":"Keesler RI, Colagross-Schouten A, Reader JR. Clinical and pathologic features of Spontaneous Klebsiella pneumoniae infection in 9 Rhesus Macaques (Macaca mulatta). Comp Med 2020; 70(2): 183\u2013189."},{"issue":"17","key":"B6","series-title":"Int J Environ Res Public Health","first-page":"6278","article-title":"The characteristic of virulence, biofilm and antibiotic resistance of Klebsiella pneumoniae","volume":"17","author":"Wang G","year":"2020","unstructured":"Wang G, Zhao G, Chao X, Xie L, Wang H. The characteristic of virulence, biofilm and antibiotic resistance of Klebsiella pneumoniae. Int J Environ Res Public Health 2020; 17(17): 6278."},{"issue":"2","key":"B7","series-title":"Antibiotics (Basel)","first-page":"234","article-title":"Carbapenem-Resistant Klebsiella pneumoniae: virulence factors, molecular epidemiology and latest updates in treatment options","volume":"12","author":"Karampatakis T","year":"2023","unstructured":"Karampatakis T, Tsergouli K, Behzadi P. Carbapenem-Resistant Klebsiella pneumoniae: virulence factors, molecular epidemiology and latest updates in treatment options. Antibiotics (Basel) 2023; 12(2): 234."},{"issue":"4","key":"B8","series-title":"Acta Biochim Pol","first-page":"867","article-title":"Klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors","volume":"62","author":"Candan ED","year":"2015","unstructured":"Candan ED, Aks\u00f6z N. Klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors. Acta Biochim Pol 2015; 62(4): 867\u2013874."},{"key":"B9","series-title":"Front Microbiol","first-page":"2436","article-title":"FNR-dependent RmpA and RmpA2 regulation of capsule polysaccharide biosynthesis in Klebsiella pneumoniae","volume":"10","author":"Lin T-H","year":"2019","unstructured":"Lin T-H, Wu C-C, Kuo J-T, Chu H-F, Lee D-Y, Lin C-T. FNR-dependent RmpA and RmpA2 regulation of capsule polysaccharide biosynthesis in Klebsiella pneumoniae. Front Microbiol 2019; 10: 2436."},{"issue":"1","key":"B10","series-title":"Infect Immun","first-page":"54","article-title":"A\u00a0gene, uge, is essential for Klebsiella pneumoniae virulence","volume":"72","author":"Regu\u00e9 M","year":"2004","unstructured":"Regu\u00e9 M, Hita B, Piqu\u00e9 N, Izquierdo L, Merino S, Fresno S, et\u00a0al. A\u00a0gene, uge, is essential for Klebsiella pneumoniae virulence. Infect Immun 2004; 72(1): 54\u201361."},{"issue":"24","key":"B11","series-title":"J Bacteriol","first-page":"7213","article-title":"The Klebsiella pneumoniae wabG gene: role in biosynthesis of the core lipopolysaccharide and virulence","volume":"185","author":"Izquierdo L","year":"2003","unstructured":"Izquierdo L, Coderch N, Piqu\u00e9 N, Bedini E, Corsaro MM, Merino S, et\u00a0al. The Klebsiella pneumoniae wabG gene: role in biosynthesis of the core lipopolysaccharide and virulence. J Bacteriol 2003; 185(24): 7213\u20137221."},{"issue":"3","key":"B12","series-title":"J Bacteriol","first-page":"1262","article-title":"Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae","volume":"171","author":"Gerlach GF","year":"1989","unstructured":"Gerlach GF, Clegg S, Allen BL. Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae. J Bacteriol 1989; 171(3): 1262\u20131270."},{"issue":"2","key":"B13","series-title":"mSphere","first-page":"e00125","article-title":"Iron acquisition and Siderophore release by Carbapenem-resistant sequence type 258 Klebsiella pneumoniae","volume":"3","author":"Holden VI","year":"2018","unstructured":"Holden VI, Wright MS, Houle S, Collingwood A, Dozois CM, Adams MD, et\u00a0al. Iron acquisition and Siderophore release by Carbapenem-resistant sequence type 258 Klebsiella pneumoniae. mSphere 2018; 3(2): e00125\u201300118."},{"issue":"1","key":"B14","series-title":"Nat Commun","first-page":"78","article-title":"Gut colonisation with multidrug-resistant Klebsiella pneumoniae worsens Pseudomonas aeruginosa lung infection","volume":"14","author":"Guern RL","year":"2023","unstructured":"Guern RL, Grandjean T, Stabler S, Bauduin M, Gosset P, Kipnis \u00c9, et\u00a0al. Gut colonisation with multidrug-resistant Klebsiella pneumoniae worsens Pseudomonas aeruginosa lung infection. Nat Commun 2023; 14(1): 78."},{"key":"B15","series-title":"Microb Pathog","first-page":"369","article-title":"Regulation of NF-\u03baB\/MAPK signaling pathway attenuates the acute lung inflammation in Klebsiella pneumonia rats by mollugin treatment","volume":"132","author":"Zhang Y","year":"2019","unstructured":"Zhang Y, Zhou S, Zhou J, Wang D, Zhou T. Regulation of NF-\u03baB\/MAPK signaling pathway attenuates the acute lung inflammation in Klebsiella pneumonia rats by mollugin treatment. Microb Pathog 2019; 132: 369\u2013373."},{"issue":"6","key":"B16","series-title":"Int J Med Microbiol","article-title":"Klebsiella pneumoniae activates the TGF-\u03b2 signaling pathway to adhere to and invade intestinal epithelial cells via enhancing TLL1 expression","volume":"312","author":"Liu J","year":"2022","unstructured":"Liu J, Zhang S, Pei H, Tu F, Liu B, Yan J, et\u00a0al. Klebsiella pneumoniae activates the TGF-\u03b2 signaling pathway to adhere to and invade intestinal epithelial cells via enhancing TLL1 expression. Int J Med Microbiol 2022; 312(6): 151561."},{"issue":"9","key":"B17","series-title":"Infect Immun","first-page":"3307","article-title":"Campylobacter jejuni translocation across intestinal epithelial cells is facilitated by ganglioside-like lipooligosaccharide structures","volume":"80","author":"Louwen R","year":"2012","unstructured":"Louwen R, Nieuwenhuis EES, Marrewijk Lv, Horst-Kreft D, Ruiter Ld, Heikema AP, et\u00a0al. Campylobacter jejuni translocation across intestinal epithelial cells is facilitated by ganglioside-like lipooligosaccharide structures. Infect Immun 2012; 80(9): 3307\u20133318."},{"issue":"12","key":"B18","series-title":"Infect Immun","first-page":"6744","article-title":"Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae","volume":"68","author":"Sahly H","year":"2000","unstructured":"Sahly H, Podschun R, Oelschlaeger TA, Greiwe M, Parolis H, Hasty D, et\u00a0al. Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae. Infect Immun 2000; 68(12): 6744\u20136749."},{"issue":"2","key":"B19","series-title":"Infect Immun","first-page":"769","article-title":"Klebsiella pneumoniae translocates across the intestinal epithelium via Rho GTPase- and phosphatidylinositol 3-kinase\/Akt-dependent cell invasion","volume":"83","author":"Hsu C-R","year":"2015","unstructured":"Hsu C-R, Pan Y-J, Liu J-Y, Chen C-T, Lin T-L, Wang J-T. Klebsiella pneumoniae translocates across the intestinal epithelium via Rho GTPase- and phosphatidylinositol 3-kinase\/Akt-dependent cell invasion. Infect Immun 2015; 83(2): 769\u2013779."},{"issue":"1","key":"B20","series-title":"Infect Immun","first-page":"44","article-title":"R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections","volume":"60","author":"Darfeuille-Michaud A","year":"1992","unstructured":"Darfeuille-Michaud A, Jallat C, Aubel D, Sirot D, Rich C, Sirot J, et\u00a0al. R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections. Infect Immun 1992; 60(1): 44\u201355."},{"issue":"3","key":"B21","series-title":"Cold Spring Harb Perspect Biol","first-page":"a004994","article-title":"Integrin structure, activation, and interactions","volume":"3","author":"Campbell ID","year":"2011","unstructured":"Campbell ID, Humphries MJ. Integrin structure, activation, and interactions. Cold Spring Harb Perspect Biol 2011; 3(3): a004994."},{"issue":"Pt 20","key":"B22","series-title":"J Cell Sci","first-page":"3563","article-title":"Integrin cytoplasmic domain-binding proteins","volume":"113","author":"Liu S","year":"2000","unstructured":"Liu S, Calderwood DA, Ginsberg MH. Integrin cytoplasmic domain-binding proteins. J Cell Sci 2000; 113(Pt 20): 3563\u20133571."},{"issue":"15","key":"B23","series-title":"Blood Adv","first-page":"4003","article-title":"Matrix stiffness controls megakaryocyte adhesion, fibronectin fibrillogenesis, and proplatelet formation through Itg\u03b23","volume":"7","author":"Guinard I","year":"2023","unstructured":"Guinard I, Nguyen T, Brassard-Jollive N, Weber J, Ruch L, Reininger L, et\u00a0al. Matrix stiffness controls megakaryocyte adhesion, fibronectin fibrillogenesis, and proplatelet formation through Itg\u03b23. Blood Adv 2023; 7(15): 4003\u20134018."},{"issue":"1","key":"B24","series-title":"Curr Opin Cell Biol","first-page":"63","article-title":"Functional cooperation between the microtubule and actin cytoskeletons","volume":"12","author":"Goode BL","year":"2000","unstructured":"Goode BL, Drubin DG, Barnes G. Functional cooperation between the microtubule and actin cytoskeletons. Curr Opin Cell Biol 2000; 12(1): 63\u201371."},{"issue":"2","key":"B25","series-title":"Nat Rev Mol Cell Biol","first-page":"142","article-title":"Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions","volume":"24","author":"Kanchanawong P","year":"2023","unstructured":"Kanchanawong P, Calderwood DA. Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions. Nat Rev Mol Cell Biol 2023; 24(2): 142\u2013161."}],"container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/71\/3\/article-p237.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/71\/3\/article-p237.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/71\/3\/article-p237.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T07:11:42Z","timestamp":1726643502000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/71\/3\/article-p237.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,18]]},"references-count":25,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/030.2024.02326","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,18]]}}}