{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:13:57Z","timestamp":1787987637262,"version":"build-2784847793"},"reference-count":18,"publisher":"Akademiai Kiado Zrt.","issue":"1","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2025,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The spread of NDM-1-harboring <jats:italic>Klebsiella pneumoniae<\/jats:italic> is a worldwide concern. In this study the whole-genome sequence (WGS) of a carbapenem- and colistin-resistant <jats:italic>K. pneumoniae<\/jats:italic> 838Gr strain is presented. This strain was isolated from a urine sample of a patient in the Intensive Care Unit (ICU) at Volos Hospital, Greece. The initial assembly produced 224 contigs with a combined genome size of 5,561,803 bp and a GC content of 57.21%. The <jats:italic>K. pneumoniae<\/jats:italic> strain carried IncR, IncFIA, IncC, and repB (R1701) replicons. Multilocus sequence typing (MLST) analysis revealed that the isolate belonged to the sequence type 11 (ST11) and serogroup KL24 and O2a. The WGS analysis identified several beta-lactamase genes (<jats:italic>bla<\/jats:italic><jats:sub>TEM-1B<\/jats:sub><jats:italic>, bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub><jats:italic>, bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub><jats:italic>, bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub><jats:italic>, bla<\/jats:italic><jats:sub>VEB-1<\/jats:sub><jats:italic>, bla<\/jats:italic><jats:sub>OXA-10<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>SHV-11<\/jats:sub>) alongside resistance genes for other antibiotic classes, including <jats:italic>floR2, cmlA1, cmlA5, catB3, arr-3, aph(6)-Id, aadA2<\/jats:italic>. Colistin resistance was attributed to specific point mutations in <jats:italic>pmrB<\/jats:italic> (R256G, T140P). This is the first report of a carbapenem- and colistin-resistant <jats:italic>K. pneumoniae<\/jats:italic> ST11 strain in Greece. The findings of this study highlight the urgent need for increased surveillance and stringent infection control.<\/jats:p>","DOI":"10.1556\/030.2025.02499","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T09:55:24Z","timestamp":1737626124000},"page":"16-22","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":7,"title":["Identification of NDM-1 producing and colistin resistant Klebsiella pneumoniae ST11: A highly drug-resistant strain detected in intensive care unit of a Greek tertiary care hospital"],"prefix":"10.1556","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2101-4546","authenticated-orcid":true,"given":"Maria","family":"Chatzidimitriou","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, International Hellenic University, 57400 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pandora","family":"Tsolakidou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital of Volos, 38222 Volos, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Anna","family":"Kyriazidi","sequence":"additional","affiliation":[{"name":"Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fani","family":"Chatzopoulou","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, International Hellenic University, 57400 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sotiris","family":"Varlamis","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, International Hellenic University, 57400 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Mavridou","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, International Hellenic University, 57400 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kallirhoe","family":"Kalinderi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, International Hellenic University, 57400 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriazis Athanasios","family":"Kyriazidis","sequence":"additional","affiliation":[{"name":"Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella","family":"Mitka","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, International Hellenic University, 57400 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"issue":"12","key":"B1","series-title":"Antimicrob Agents Chemother","first-page":"5046","article-title":"Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India","volume":"53","author":"Yong D","year":"2009 Dec","unstructured":"Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et\u00a0al. 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