{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T04:06:58Z","timestamp":1743134818690,"version":"3.40.3"},"reference-count":15,"publisher":"Akademiai Kiado Zrt.","issue":"1","funder":[{"DOI":"10.13039\/100022833","name":"Rajiv Gandhi Centre for Biotechnology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100022833","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2025,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infections caused by colistin resistant <jats:italic>Klebsiella pneumoniae<\/jats:italic> are a major global health challenge linked to high mortality rates worldwide. Increased incidence of hypervirulent and drug-resistant <jats:italic>Klebsiella<\/jats:italic> causing life-threatening infections in young healthy individuals and asymptomatic carriage in the community has been largely reported in the Asian-Pacific Rim. This study conducted a molecular analysis of two morphologically distinct variants of <jats:italic>K. pneumoniae<\/jats:italic> that caused bacteremia and sepsis in a patient. Colony morphology of the isolates was characterized in various growth media, and the morphological variants differed in their mucoviscosity. The isolates were found to be serotype K2 (highly associated with hypervirulent <jats:italic>Klebsiella<\/jats:italic>) by molecular serotyping using specific PCR primers. The multidrug-resistant nature of the colony variants was evaluated by antibiotic susceptibility testing and it was found to have a similar antibiogram pattern in <jats:italic>in vitro<\/jats:italic>. An increased minimum inhibitory concentration (MIC) of colistin (&gt;64\u2009\u03bcg mL<jats:sup>\u22121<\/jats:sup>) was detected in both isolates using broth microdilution, and they were found to be highly resistant to colistin. Molecular analysis revealed that the isolates possessed a chromosomal mutation in <jats:italic>mgrB<\/jats:italic>, which causes colistin resistance. The increased incidence of infection caused by colistin-resistant <jats:italic>K. pneumoniae<\/jats:italic> requires continuous monitoring, and appropriate measures are necessary to control its adaptive evolution in healthcare settings.<\/jats:p>","DOI":"10.1556\/030.2025.02515","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T14:01:37Z","timestamp":1742479297000},"page":"39-42","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Detection of morphological variants of colistin-resistant Klebsiella pneumoniae associated with sepsis in Kerala, India"],"prefix":"10.1556","volume":"72","author":[{"given":"Merin","family":"Paul","sequence":"first","affiliation":[{"name":"Pathogen Biology Group, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695014, Kerala, India"},{"name":"Research Centre, University of Kerala, Thiruvananthapuram 695034, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7634-8930","authenticated-orcid":true,"given":"Sabu","family":"Thomas","sequence":"additional","affiliation":[{"name":"Pathogen Biology Group, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695014, Kerala, India"},{"name":"Centre of Excellence in Microbiome -KSCSTE, KINFRA Film and Video Park, Kazhakkootam, Thiruvananthapuram 695585, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"issue":"3","key":"B1","series-title":"Lancet Infect Dis","first-page":"318","article-title":"WHO Pathogens Priority List Working Group. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli E","year":"2018","unstructured":"Tacconelli E, Carrara E, Savoldi A, Harbarth S, Mendelson M, Monnet DL, et al. WHO Pathogens Priority List Working Group. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis 2018; 18(3): 318\u2013327. https:\/\/doi.org\/10.1016\/S1473-3099(17)30753-3."},{"issue":"6","key":"B2","series-title":"Lancet Infect Dis","first-page":"761","article-title":"Global evolution and geographic diversity of hypervirulent carbapenem-resistant Klebsiella pneumoniae","volume":"22","author":"Wu Y","year":"2022","unstructured":"Wu Y, Wu C, Bao D, Jia H, Draz MS, He F, et al. Global evolution and geographic diversity of hypervirulent carbapenem-resistant Klebsiella pneumoniae. Lancet Infect Dis 2022; 22(6): 761\u2013762. https:\/\/doi.org\/10.1016\/S1473-3099(22)00275-4."},{"key":"B3","series-title":"Front Microbiol","article-title":"Colistin resistance among multiple sequence types of Klebsiella pneumoniae is associated with diverse resistance mechanisms: a report from India","volume":"12","author":"Azam M","year":"2021","unstructured":"Azam M, Gaind R, Yadav G, Sharma A, Upmanyu K, Jain M, et al. Colistin resistance among multiple sequence types of Klebsiella pneumoniae is associated with diverse resistance mechanisms: a report from India. Front Microbiol 2021; 12: 609840. https:\/\/doi.org\/10.3389\/fmicb.2021.609840."},{"issue":"6","key":"B4","series-title":"J Infect Dev Ctries","first-page":"606","article-title":"Clinical and microbiological characteristics of hypervirulent Klebsiella pneumoniae (hvKp) in a hospital from North China","volume":"14","author":"Yang Y","year":"2020","unstructured":"Yang Y, Liu JH, Hu XX, Zhang W, Nie TY, Yang XY, et al. 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Antimicrob Agents Chemother 2016; 60(10): 6115\u20136120. https:\/\/doi.org\/10.1128\/AAC.01127-16."},{"issue":"2","key":"B6","series-title":"Lancet Infect Dis","first-page":"161","article-title":"Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study","volume":"16","author":"Liu YY","year":"2016","unstructured":"Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016; 16(2): 161\u2013168. https:\/\/doi.org\/10.1016\/S1473-3099(15)00424-7."},{"key":"B7","series-title":"Front Microbiol","first-page":"2470","article-title":"MgrB alterations mediate Colistin resistance in Klebsiella pneumoniae isolates from Iran","volume":"8","author":"Haeili M","year":"2017","unstructured":"Haeili M, Javani A, Moradi J, Jafari Z, Feizabadi MM, Babaei E. MgrB alterations mediate Colistin resistance in Klebsiella pneumoniae isolates from Iran. Front Microbiol 2017; 8: 2470. https:\/\/doi.org\/10.3389\/fmicb.2017.02470."},{"issue":"4","key":"B8","series-title":"Indian J Med Microbiol","first-page":"593","article-title":"Emerging atypical non-lactose-fermenting phenotypic variants of Klebsiella pneumoniae and Escherichia coli in admitted patients of a trauma centre","volume":"37","author":"Bajpai V","year":"2019","unstructured":"Bajpai V, Mathur P. Emerging atypical non-lactose-fermenting phenotypic variants of Klebsiella pneumoniae and Escherichia coli in admitted patients of a trauma centre. Indian J Med Microbiol 2019; 37(4): 593\u2013594. https:\/\/doi.org\/10.4103\/ijmm.IJMM_20_61."},{"issue":"3","key":"B9","series-title":"Int J Mol Sci","first-page":"1944","article-title":"Hypervirulent capsular serotypes K1 and K2 Klebsiella pneumoniae strains demonstrate resistance to serum bactericidal activity and Galleria mellonella lethality","volume":"25","author":"Al-Busaidi B","year":"2024","unstructured":"Al-Busaidi B, Al-Muzahmi M, Al-Shabibi Z, Rizvi M, Al-Rashdi A, Al-Jardani A, et al. Hypervirulent capsular serotypes K1 and K2 Klebsiella pneumoniae strains demonstrate resistance to serum bactericidal activity and Galleria mellonella lethality. Int J Mol Sci 2024; 25(3): 1944. https:\/\/doi.org\/10.3390\/ijms25031944."},{"issue":"5","key":"B10","series-title":"Mol Microbiol","first-page":"883","article-title":"Deciphering the role of the capsule of Klebsiella pneumoniae during pathogenesis: a cautionary tale","volume":"113","author":"Rendueles O.","year":"2020","unstructured":"Rendueles O. Deciphering the role of the capsule of Klebsiella pneumoniae during pathogenesis: a cautionary tale. Mol Microbiol 2020; 113(5): 883\u2013888. https:\/\/doi.org\/10.1111\/mmi.14474."},{"issue":"5","key":"B11","series-title":"Mol Microbiol","first-page":"889","article-title":"Cell envelope defects of different capsule-null mutants in K1 hypervirulent Klebsiella pneumoniae can affect bacterial pathogenesis","volume":"113","author":"Tan YH","year":"2020","unstructured":"Tan YH, Chen Y, Chu WHW, Sham LT, Gan YH. Cell envelope defects of different capsule-null mutants in K1 hypervirulent Klebsiella pneumoniae can affect bacterial pathogenesis. Mol Microbiol 2020; 113(5): 889\u2013905. https:\/\/doi.org\/10.1111\/mmi.14447."},{"key":"B12","series-title":"J Glob Antimicrob Resist","first-page":"187","article-title":"Genome sequence of a multidrug-resistant Klebsiella pneumoniae ST78 with high colistin resistance isolated from a patient in India","volume":"17","author":"Paul M","year":"2019","unstructured":"Paul M, Narendrakumar L, R Vasanthakumary A, Joseph I, Thomas S. Genome sequence of a multidrug-resistant Klebsiella pneumoniae ST78 with high colistin resistance isolated from a patient in India. 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Pharmaceuticals (Basel) 2023; 16(11): 1615. https:\/\/doi.org\/10.3390\/ph16111615."}],"container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/72\/1\/article-p39.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/72\/1\/article-p39.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/72\/1\/article-p39.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T16:23:55Z","timestamp":1743092635000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/72\/1\/article-p39.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,27]]},"references-count":15,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1556\/030.2025.02515","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"type":"print","value":"1217-8950"},{"type":"electronic","value":"1588-2640"}],"subject":[],"published":{"date-parts":[[2025,3,27]]}}}