{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T04:06:54Z","timestamp":1743134814544,"version":"3.40.3"},"reference-count":38,"publisher":"Akademiai Kiado Zrt.","issue":"1","funder":[{"name":"Kocaeli University Scientific Research Projects Coordination Unit"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2025,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic> is a significant nosocomial pathogen recognized for its multidrug-resistance (MDR) and capacity to endure in hospital settings. This study aims to investigate the clonal relationships of <jats:italic>A. baumannii<\/jats:italic> isolates from diverse clinical samples, identify the sequence types of MDR\u00a0isolates, and examine biofilm formation activity and biofilm-associated genes that contribute to persistence in hospital settings. A total of 90 <jats:italic>A. baumannii<\/jats:italic> isolates were analyzed. Bacterial identification and antibiotic susceptibility testing were conducted with MALDI-TOF MS and Vitek-2. REP-PCR was utilized to evaluate clonal connections, MLST was employed for specific isolates. Biofilm formation activity was assessed using the XTT reduction assay, and biofilm-associated genes were identified by PCR. REP-PCR revealed 29 genotypes, with Genotype A being identified as the endemic clone in 59% of isolates. Two isolates representing this genotype were found to belong to the ST2 clone. The majority of <jats:italic>A. baumannii<\/jats:italic> isolates possess biofilm-related genes and exhibit strong biofilm activity. In MDR isolates, <jats:italic>ompA<\/jats:italic> and <jats:italic>csuE<\/jats:italic> positivity were significantly higher than those non-MDR isolates (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003, <jats:italic>P<\/jats:italic> = 0.001). The c<jats:italic>suE<\/jats:italic> positive isolates were found to have significantly stronger biofilm activity than negative ones (<jats:italic>P<\/jats:italic> = 0.009). This study emphasizes the prevalence of a hospital-endemic, MDR <jats:italic>A. baumannii<\/jats:italic> genotype A, ST2 clone, and the genetic variability across isolates. No direct correlation was noted between MDR status and biofilm formation; however, some biofilm-related genes, notably <jats:italic>csuE<\/jats:italic>, were linked to stronger biofilm activity. These findings underscore the necessity for\u00a0ongoing molecular surveillance and infection control measures to avert the dissemination of MDR <jats:italic>A. baumannii<\/jats:italic> in healthcare environments.<\/jats:p>","DOI":"10.1556\/030.2025.02531","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T08:21:26Z","timestamp":1742804486000},"page":"33-38","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Genotypic analysis and biofilm formation of\u00a0Acinetobacter baumannii clinical isolates"],"prefix":"10.1556","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7433-7285","authenticated-orcid":true,"given":"Fatma Zehra","family":"Duymaz","sequence":"first","affiliation":[{"name":"Necip Faz\u0131l City Hospital, Medical Microbiology, Kahramanmaras, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8439-3881","authenticated-orcid":true,"given":"Fatma","family":"Budak","sequence":"additional","affiliation":[{"name":"Medical Microbiology Department, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2191-6651","authenticated-orcid":true,"given":"Elif","family":"Okumu\u015f","sequence":"additional","affiliation":[{"name":"Van Training and Research Hospital, Medical Microbiology, Van, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"B1","series-title":"APMIS","first-page":"330","article-title":"Acinetobacter baumannii: insights towards a comprehensive approach for the prevention of outbreaks in health\u2010care facilities","volume":"130","author":"Duarte DFB","year":"2022","unstructured":"Duarte DFB, Rodrigues G. 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Int J Infect Dis 2016; 52: 29\u201336."},{"key":"B38","series-title":"Antimicrob Agents Chemother","first-page":"3602","article-title":"High prevalence of bla(NDM-1) carbapenemase-encoding gene and 16S rRNA armA methyltransferase gene among Acinetobacter baumannii clinical Isolates in Egypt","volume":"59","author":"El-Sayed-Ahmed MAE-G","year":"2015","unstructured":"El-Sayed-Ahmed MAE-G, Amin MA, Tawakol WM, Loucif L, Bakour S, Rolain J-M. High prevalence of bla(NDM-1) carbapenemase-encoding gene and 16S rRNA armA methyltransferase gene among Acinetobacter baumannii clinical Isolates in Egypt. 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