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During the two-year study period, 42 VISA were obtained from 520\u2009<jats:italic>S.\u00a0aureus<\/jats:italic> isolates collected from various clinical samples, corresponding to a prevalence of 8.1%, as determined by the broth microdilution method. These VISA isolates were further characterized using biofilm formation, antimicrobial susceptibility tests, <jats:italic>SCCmec<\/jats:italic> typing, <jats:italic>spa<\/jats:italic> typing, multilocus sequence typing (MLST), and PCR analysis for detecting resistance (<jats:italic>erm(B), tet(M), mecC, msr(B), mecA, mupA, vanA, aac(6\u2032)-Ie\/aph(2\u02dd), mupB, msr(A), erm(C), erm(A), vanB, ant(4\u2032)-Ia, and aph(3\u2032)-IIIa<\/jats:italic>), biofilm (<jats:italic>clfA, clfB, fnbA, fnbB, ebp, cna<\/jats:italic> and <jats:italic>bap<\/jats:italic>) and virulence <jats:italic>(eta<\/jats:italic>, <jats:italic>etb, pvl<\/jats:italic>, and <jats:italic>tst<\/jats:italic>) genes. Our results indicated that the 42 VISA isolates belonged to three clonal complexes, including CC8 (78.6%), CC22 (11.9%), and CC5 (9.5%). The vast majority of <jats:italic>S. aureus<\/jats:italic> isolates belonged to CC8\/ST239-SCC<jats:italic>mec<\/jats:italic> III\/t037 (42.9%). Our result revealed that PVL-positive strains belonged to CC\/ST5-SCC<jats:italic>mec<\/jats:italic> IV\/t002 (9.5%), CC\/ST8-SCC<jats:italic>mec<\/jats:italic> IV\/t008 (19%), and CC\/ST22-SCC<jats:italic>mec<\/jats:italic> IV\/t790 (7.1%) while TST-positive isolates belonged to CC8\/ST239-SCC<jats:italic>mec<\/jats:italic> III\/t030 (9.5%) and CC8\/ST239-SCC<jats:italic>mec<\/jats:italic> III\/t037 (35.7%). The majority of HLMUPR isolates belonged to CC8\/ST239-SCC<jats:italic>mec<\/jats:italic> III\/t037 (14.3%), followed by CC\/ST8-SCC<jats:italic>mec<\/jats:italic> IV\/t008 (7.1%), CC8\/ST239-SCC<jats:italic>mec<\/jats:italic> III\/t030 (4.8%), and CC\/ST5-SCC<jats:italic>mec<\/jats:italic> IV\/t002 (2.4%) lineages carrying <jats:italic>mupA<\/jats:italic>. The highest frequency of VISA strain with iMLSB phenotype belonged to the CC8\/ST239-SCC<jats:italic>mec<\/jats:italic> III\/t037 (11.9%) clonal lineage. The study highlights that genetic diversity and characteristics of the VISA strains should be closely and continuously monitored. Besides that, importance of measures to prevent the transmission of VISA to treat such infection were urgently needed.<\/jats:p>","DOI":"10.1556\/030.2025.02639","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:15:37Z","timestamp":1757589337000},"page":"237-247","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Genetic characteristics of vancomycin intermediate Staphylococcus aureus isolated from patients in Tehran, Iran: CC\/ST8 is\u00a0a\u00a0serious threat"],"prefix":"10.1556","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6720-9341","authenticated-orcid":true,"given":"Mehdi","family":"Goudarzi","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mozhgan","family":"Raigani","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Salehi","sequence":"additional","affiliation":[{"name":"Department of Mycology, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masoumeh","family":"Navidinia","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Javad","family":"Nasiri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Goudarzi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"B1","series-title":"Rev Res Med Microbiol","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1097\/MRM.0000000000000067","article-title":"A review of virulence factors, pathogenesis, and antibiotic resistance in Staphylococcus aureus","volume":"27","author":"Al-Mebairik NF","year":"2016","unstructured":"Al-Mebairik NF, El-Kersh TA, Al-Sheikh YA, Marie MAM. 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