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The objective of this study was to identify the mutations responsible for conferring resistance to rifampicin (RIF) and isoniazid (INH) in\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    . A total of 84 drug-resistant\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    strains including 41 multidrug-resistant (MDR) strains, 37 INH-resistant, RIF-susceptible strains, and 6 RIF-resistant, INH-susceptible strains were analyzed. The 86\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    strains were isolated from clinical samples, between 2022 and 2023 in Ankara, Turkey. PCR amplification and sequencing of\n                    <jats:italic>rpoB<\/jats:italic>\n                    ,\n                    <jats:italic>katG<\/jats:italic>\n                    and\n                    <jats:italic>inhA<\/jats:italic>\n                    genes were performed to detect mutations. In the 47 RIF-resistant strains, the predominant mutation in\n                    <jats:italic>rpoB<\/jats:italic>\n                    was S450L observed in 40 of 47 strains (85%), followed by H445Y detected in two strains (4.3%). The Q432K\/P, M434I, D435Y, T444I, H445G, S450W, and S450F mutations were identified in one strain each. The S315T mutation in the\n                    <jats:italic>katG<\/jats:italic>\n                    gene was identified in 60 of the 78 INH-resistant strains (76.9%). The rate of mutation \u221215C&gt;T in\n                    <jats:italic>inhA<\/jats:italic>\n                    was 29.5% (23\/78). Both S315Y and \u221215C&gt;T mutations were detected in six strains (7.7%). This study provided comprehensive information regarding the genetic background of drug-resistant tuberculosis in terms of prevalent mutations responsible for RIF and INH resistance. These findings contribute to develop more sensitive qPCR tests that target mutations for the rapid detection of drug-resistant TB.\n                  <\/jats:p>","DOI":"10.1556\/030.2026.02818","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T07:55:59Z","timestamp":1768809359000},"page":"95-100","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":0,"title":["Mutations related to rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains in Ankara, Turkey"],"prefix":"10.1556","volume":"73","author":[{"given":"Oguz","family":"Ari","sequence":"first","affiliation":[{"name":"Ankara Yildirim Beyazit University, Central Research and Application Center, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6561-778X","authenticated-orcid":true,"given":"Riza","family":"Durmaz","sequence":"additional","affiliation":[{"name":"Ankara Yildirim Beyazit University, Faculty of Medicine, Department of Clinical Microbiology, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Arslanturk","sequence":"additional","affiliation":[{"name":"Ministry of Health, General Directorate of Public Health, Microbiology Reference Laboratories and Biological Products Department, National Tuberculosis Reference Laboratory, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sedat","family":"Vezir","sequence":"additional","affiliation":[{"name":"Ankara Atat\u00fcrk Sanatory Education and Research Hospital, Department of Clinical Microbiology, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","reference":[{"key":"B1","series-title":"Cureus","article-title":"Treatment and toxicity considerations in tuberculosis: a narrative review","volume":"16","author":"Jones NT","year":"2024","unstructured":"Jones NT, Abadie R, Keller CL, Jones K, Ledet Iii LF, Fox JE, et al. 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