{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T10:21:49Z","timestamp":1789813309775,"version":"4.0.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Microbiologica et Immunologica Hungarica"],"published-print":{"date-parts":[[2015,9]]},"abstract":"We investigated the molecular epidemiology of extended spectrum \u03b2-lactamase (ESBL) producing Klebsiella pneumoniae<\/jats:italic> isolates derived from the teaching hospitals of University of P\u00e9cs, P\u00e9cs, Hungary in the time period 2004\u20132008. Molecular typing, antimicrobial susceptibility testing, detection of common \u03b2-lactamase genes (bla<\/jats:italic>CTX-M<\/jats:sub>, bla<\/jats:italic>TEM<\/jats:sub> and bla<\/jats:italic>SHV<\/jats:sub>) and virulence associated traits (hypermucoviscosity, magA<\/jats:italic>, k2a<\/jats:italic>, rmpA<\/jats:italic>, siderophores, type 1 and 3 fimbria, biofilm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofloxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identified, and were shown to harbour the following \u03b2-lactamase genes: six clones carried bla<\/jats:italic>CTX-M<\/jats:sub>, four clones harboured bla<\/jats:italic>SHV-5<\/jats:sub> and one clone possessed both bla<\/jats:italic>CTX-M<\/jats:sub> and ESBL type bla<\/jats:italic>SHV<\/jats:sub>. Among the SHV-5 producing K. pneumoniae<\/jats:italic> clones a novel sequence type was found, namely ST1193, which harboured a unique infB<\/jats:italic> allele. Different virulence factor content and peculiar antimicrobial susceptibility profile were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofloxacin, minor clone isolates displayed significantly lower MIC values for ciprofloxacin suggesting a role for fluoroquinolones in the dissemination of the major K. pneumoniae<\/jats:italic> clones. This is the first description of the CTX-M-15 producing K. pneumoniae<\/jats:italic> clone ST101 in Hungary.<\/jats:p>","DOI":"10.1556\/030.62.2015.3.2","type":"journal-article","created":{"date-parts":[[2015,9,24]],"date-time":"2015-09-24T04:20:30Z","timestamp":1443068430000},"page":"233-245","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":15,"title":["Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital"],"prefix":"10.1556","volume":"62","author":[{"given":"Szilvia","family":"Melegh","sequence":"first","affiliation":[{"name":"1Department of Medical Microbiology and Immunology, Clinical Centre, University of P\u00e9cs, P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6rgy","family":"Schneider","sequence":"additional","affiliation":[{"name":"1Department of Medical Microbiology and Immunology, Clinical Centre, University of P\u00e9cs, P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"1Department of Medical Microbiology and Immunology, Clinical Centre, University of P\u00e9cs, P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferenc","family":"Jakab","sequence":"additional","affiliation":[{"name":"2Virological Research Group, Szent\u00e1gothai Research Centre, University of P\u00e9cs, P\u00e9cs, Hungary"},{"name":"3Institute of Biology, Faculty of Sciences, University of P\u00e9cs, P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levente","family":"Em\u0151dy","sequence":"additional","affiliation":[{"name":"1Department of Medical Microbiology and Immunology, Clinical Centre, University of P\u00e9cs, P\u00e9cs, Hungary"},{"name":"4Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Tigyi","sequence":"additional","affiliation":[{"name":"1Department of Medical Microbiology and Immunology, Clinical Centre, University of P\u00e9cs, P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/62\/3\/article-p233.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/62\/3\/article-p233.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:51:11Z","timestamp":1614599471000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/62\/3\/article-p233.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/030.62.2015.3.2","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]}}}