{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T13:07:08Z","timestamp":1781269628468,"version":"3.54.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"4","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Microbiologica et Immunologica Hungarica"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Brucellosis is a zoonosis of economic importance that reduces productivity in livestock enterprises as it induces abortion in infected animals. A study was designed aimed at detecting <jats:italic>Brucella<\/jats:italic> in blood and lymph node specimens from camels by the use of real-time PCR in Iran. Sample collection and DNA extraction were done on blood (n = 135) and lymph node (n = 135) samples collected from 135 camels (abattoir survey) from both sexes at various ages in different seasons. The real-time PCR for species differentiation was based on unique genetic loci of <jats:italic>B. melitensis<\/jats:italic> and <jats:italic>B. abortus<\/jats:italic>. The regions were chosen for the construction of primers and TaqMan\u00ae probes for species differentiation: BMEII0466 gene for <jats:italic>B. melitensis<\/jats:italic> and Bru-Ab2_0168 gene for <jats:italic>B. abortus. Brucella<\/jats:italic> spp. were identified in 18 (13.33%) blood samples and 4 (2.97%) lymph node samples. This method showed to be effective in detecting <jats:italic>B. abortus<\/jats:italic> and <jats:italic>B. melitensis<\/jats:italic> in blood and lymph samples respectively. <jats:italic>Brucella abortus<\/jats:italic> was detected in 3 (2.22%) blood samples but was however, not detected in the lymph node samples. <jats:italic>Brucella melitensis<\/jats:italic> was only observed in 4 (2.97%) lymph node samples. Significant differences were observed on the blood prevalence of unknown <jats:italic>Brucella<\/jats:italic> spp. in different age groups and seasons (P &lt; 0.05). However, there were no significant differences observed on the prevalence of <jats:italic>B. abortus<\/jats:italic>, <jats:italic>B. melitensis<\/jats:italic>, unknown <jats:italic>Brucella<\/jats:italic> spp. in different age groups, sex and seasons (P &gt; 0.05). Therefore, <jats:italic>Brucella<\/jats:italic> was detected in apparent healthy camels slaughtered at an abattoir in Iran and this recommends the significance of the detection of <jats:italic>Brucella<\/jats:italic> in camels, since the infected camels appear to be healthy.<\/jats:p>","DOI":"10.1556\/030.62.2015.4.6","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T18:51:06Z","timestamp":1450723866000},"page":"409-421","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":8,"title":["Molecular study of Brucellosis in camels by the use of TaqMan\u00ae real-time PCR"],"prefix":"10.1556","volume":"62","author":[{"given":"Faham","family":"Khamesipour","sequence":"first","affiliation":[{"name":"1Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Doosti","sequence":"additional","affiliation":[{"name":"2Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebrahim","family":"Rahimi","sequence":"additional","affiliation":[{"name":"3Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/62\/4\/article-p409.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/62\/4\/article-p409.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:51:34Z","timestamp":1614599494000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/62\/4\/article-p409.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/030.62.2015.4.6","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12]]}}}