{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T18:19:58Z","timestamp":1784657998523,"version":"3.55.0"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Microbiologica et Immunologica Hungarica"],"published-print":{"date-parts":[[2016,8,18]]},"abstract":"<jats:p>The purpose of this study was to determine prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes [<jats:italic>qnrA, qnrB, qnrC, qnrD, qnrS, aac(6\u2032)-Ib-cr, qepA,<\/jats:italic> and <jats:italic>oqxAB<\/jats:italic>] among extended-spectrum beta-lactamase (ESBL)-producing <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Klebsiella<\/jats:italic> spp. isolates from bloodcultures in Hungary. A total of 103 isolates were tested for quinolone susceptibility by microdilution method and PMQR genes were detected by polymerase chain reaction. About 40 ESBL-producing <jats:italic>E. coli<\/jats:italic> (39%) and 50 ESBL-producing <jats:italic>Klebsiella<\/jats:italic> spp. strains (48%) were resistant to ciprofloxacin; 40 ESBL-producing <jats:italic>E. coli<\/jats:italic> (39%) and 47 ESBL-producing <jats:italic>Klebsiella<\/jats:italic> spp. strains (45%) were resistant to levofloxacin; and 88 strains including 40 ESBL-producing <jats:italic>E. coli<\/jats:italic> (39%) and 48 (47%) ESBL-producing <jats:italic>Klebsiella<\/jats:italic> spp. were resistant to moxifloxacin. Among the 103 ESBL-producing isolates, 77 (75%) isolates (30 <jats:italic>E. coli<\/jats:italic> and 47 <jats:italic>Klebsiella<\/jats:italic> spp.) harbored PMQR genes. The most commonly detected gene was <jats:italic>aac(6\u2032)-Ib-cr<\/jats:italic> (65%). The occurrence of <jats:italic>qnrS<\/jats:italic> gene was 6%. Interestingly, <jats:italic>qnrA, qnrB, qnrC, qnrD<\/jats:italic>, and <jats:italic>qepA<\/jats:italic> were not found in any isolates. Among 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried two and three different PMQR genes, respectively. Only <jats:italic>Klebsiella<\/jats:italic> spp. harbored more than one PMQR genes. Observing prevalence of PMQR genes in the last 8 years, the increasing incidence of <jats:italic>aac(6\u2032)-Ib-cr<\/jats:italic> and <jats:italic>oqxAB<\/jats:italic> can be seen. Our results highlight high frequency of PMQR genes among ESBL-producing <jats:italic>Klebsiella pneumoniae<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> isolates with an increasing dynamics in Hungary.<\/jats:p>","DOI":"10.1556\/030.63.2016.002","type":"journal-article","created":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T08:01:11Z","timestamp":1471593671000},"page":"313-323","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":14,"title":["Plasmid-mediated quinolone resistance among extended spectrum beta lactase producing Enterobacteriaceae from bloodstream infections"],"prefix":"10.1556","volume":"63","author":[{"given":"Judit","family":"Domokos","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Krist\u00f3f","sequence":"additional","affiliation":[{"name":"Labor Medicine Institute, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00f3ra","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/63\/3\/article-p313.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/63\/3\/article-p313.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/63\/3\/article-p313.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T15:38:53Z","timestamp":1704296333000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/63\/3\/article-p313.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,18]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/030.63.2016.002","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,18]]}}}