{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:34:18Z","timestamp":1792802058588,"version":"4.5.0"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Microbiologica et Immunologica Hungarica"],"published-print":{"date-parts":[[2016,12,15]]},"abstract":"<jats:p>\n          <jats:italic>Acinetobacter baumannii<\/jats:italic> is a major opportunistic pathogen in healthcare settings worldwide. In Iran, there are only few reports on the prevalence of aminoglycoside resistance genes among <jats:italic>A. baumannii<\/jats:italic> isolates. The aim of this study was to investigate the existence of aminoglycoside-modifying enzyme (AME) genes from <jats:italic>A. baumannii<\/jats:italic> strains collected at a university teaching hospital in Iran. One hundred <jats:italic>A. baumannii<\/jats:italic> strains were collected between 2014 and 2015 from hospitalized patients at Loghman Hakim Hospital, Tehran, Iran. Antimicrobial susceptibility was determined by disk diffusion method according to the Clinical and Laboratory Standards Institute recommendations. The DNA was extracted using a kit obtained from Bioneer Co. (Korea) and was used as a template for polymerase chain reaction. The most active antimicrobial agent against these strains was colistin. The rate of extended-spectrum cephalosporin resistance was 97%. The <jats:italic>aadA1<\/jats:italic>, <jats:italic>aadB<\/jats:italic>, <jats:italic>aac(6\u2032)-Ib<\/jats:italic>, and <jats:italic>aac(3)-IIa<\/jats:italic> genes were found in 85%, 77%, 72%, and 68% of <jats:italic>A. baumannii<\/jats:italic> isolates, respectively. This study showed a high prevalence rate of AME genes in <jats:italic>A. baumannii.<\/jats:italic> This prevalence rate has explained that further aminoglycoside resistance genes may have role in the resistance of clinical isolates of <jats:italic>A. baumannii<\/jats:italic>. Therefore, control and treatment of serious infections caused by this opportunistic pathogen should be given more consideration.<\/jats:p>","DOI":"10.1556\/030.63.2016.022","type":"journal-article","created":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T12:29:14Z","timestamp":1481891354000},"page":"143-150","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":9,"title":["Molecular detection of aminoglycoside-modifying enzyme genes in Acinetobacter baumannii clinical isolates"],"prefix":"10.1556","volume":"64","author":[{"given":"Mohsen","family":"Heidary","sequence":"first","affiliation":[{"name":"1 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Salimi Chirani","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Khoshnood","sequence":"additional","affiliation":[{"name":"3 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gita","family":"Eslami","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyyed Mohammad","family":"Atyabi","sequence":"additional","affiliation":[{"name":"4 Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habibollah","family":"Nazem","sequence":"additional","affiliation":[{"name":"5 Department of Biology, Payame Noor University, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Fazilati","sequence":"additional","affiliation":[{"name":"5 Department of Biology, Payame Noor University, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hashemi","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleh","family":"Soleimani","sequence":"additional","affiliation":[{"name":"5 Department of Biology, Payame Noor University, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/030.63.2016.022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/64\/2\/article-p143.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/64\/2\/article-p143.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:51:41Z","timestamp":1614599501000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/64\/2\/article-p143.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,15]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/030.63.2016.022","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,15]]}}}