{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:05:35Z","timestamp":1790687135339,"version":"4.1.0"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Microbiologica et Immunologica Hungarica"],"published-print":{"date-parts":[[2017,4,17]]},"abstract":"<jats:p>Resistance to \u03b2-lactams in <jats:italic>Enterobacteriaceae<\/jats:italic> has been increasing worldwide. This study aimed to determine the frequency of \u03b2-lactamase genes and antibiotic resistance rates of 140 extended-spectrum \u03b2-lactamase (ESBL)-producing <jats:italic>Escherichia coli<\/jats:italic> isolates obtained from urinary tract infection in Ordu Province, Turkey. Isolates were identified by classic methods and by automated system. ESBL production was confirmed by double disk synergy test and antimicrobial susceptibility was investigated by disk diffusion method. All isolates were screened for \u03b2-lactamase coding genes from three groups (A, B, and D) by polymerase chain reaction. The highest rate of susceptible isolates was observed for imipenem (IPM, 99.3%) and ertapenem (ETP, 97.9%), and the highest rate of resistant isolates was observed for cefuroxime (97.9%), ceftriaxone (97.2%), and cefazolin (90.7%). In our study, <jats:italic>bla<\/jats:italic><jats:sub>CTX-M1-like<\/jats:sub> group was the most prevalent \u03b2-lactamase (<jats:italic>n<\/jats:italic>\u2009=\u2009109), followed by <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u200968), <jats:italic>bla<\/jats:italic><jats:sub>CTX-M2 <\/jats:sub>(<jats:italic>n<\/jats:italic>\u2009=\u200922), and <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20092). By contrast to low resistance rate to IPM and ETP, we determined <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub> in 31 isolates (22.1%). In co-prevalence of <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> and ESBL-coding genes, a low carbapenem resistance was determined. We can confirm that <jats:italic>bla<\/jats:italic><jats:sub>CTX-M1-types<\/jats:sub> are still the most frequent \u03b2-lactamase coding gene in Turkey. Our study showed the highest prevalence of <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> metallo-\u03b2-lactamase coding gene in ESBL-producing <jats:italic>E. coli<\/jats:italic>.<\/jats:p>","DOI":"10.1556\/030.63.2016.027","type":"journal-article","created":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T07:39:10Z","timestamp":1492587550000},"page":"131-141","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":5,"title":["High prevalence of NDM metallo-\u03b2-lactamase among ESBL-producing Escherichia coli \u0130solates"],"prefix":"10.1556","volume":"64","author":[{"given":"Yeliz","family":"\u00c7etinkol","sequence":"first","affiliation":[{"name":"1 Department of Medical Microbiology, Faculty of Medicine, Ordu University, Ordu, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cemal","family":"Sandalli","sequence":"additional","affiliation":[{"name":"2 Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdo\u011fan University, Rize, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Kerem","family":"\u00c7algin","sequence":"additional","affiliation":[{"name":"1 Department of Medical Microbiology, Faculty of Medicine, Ordu University, Ordu, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arzu Altun\u00e7eki\u00e7","family":"Yildirim","sequence":"additional","affiliation":[{"name":"3 Department of Infectious Diseases, Faculty of Medicine, Ordu University, Ordu, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esma","family":"Akyildiz","sequence":"additional","affiliation":[{"name":"2 Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdo\u011fan University, Rize, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esin","family":"Karaman","sequence":"additional","affiliation":[{"name":"2 Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdo\u011fan University, Rize, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ay\u015feg\u00fcl \u00c7opur","family":"\u00c7i\u00e7ek","sequence":"additional","affiliation":[{"name":"4 Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdo\u011fan University, Rize, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/030.63.2016.027","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/64\/2\/article-p131.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/64\/2\/article-p131.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:51:45Z","timestamp":1614599505000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/64\/2\/article-p131.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,17]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/030.63.2016.027","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,17]]}}}