{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:51:23Z","timestamp":1780570283848,"version":"3.54.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Microbiologica et Immunologica Hungarica"],"published-print":{"date-parts":[[2017,5,30]]},"abstract":"<jats:p>The modulation of efflux pump functions under fluoroquinolone (FQ) exposure is of great concern as it could result in occurrence of multidrug-resistant (MDR) bacterial strains. In this study, MDR mechanism in <jats:italic>Pseudomonas aeruginosa<\/jats:italic> induced via moxifloxacin (MOX) pressure was investigated. After serial MOX [concentration of 0.5\u2009\u00d7\u2009the minimum inhibitory concentration (MIC)] exposure, the fully susceptible <jats:italic>P. aeruginosa<\/jats:italic> ATCC 9027 strain has increased its MIC not only toward MOX (1\u2192128\u00a0mg\/L) but also to other antibiotics. Furthermore, this MOX-exposed strain did not revert to antibiotic-sensitive phenotype when being cultured in antibiotic-free medium for 12 days. No mutation was observed for FQ-target (<jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic>) or most investigated efflux regulatory genes (<jats:italic>mexT<\/jats:italic>, <jats:italic>mexR<\/jats:italic>, and <jats:italic>nalC<\/jats:italic>) except <jats:italic>nfxB<\/jats:italic> in which a 100-bp deletion was found. This associated with the elevated expression of multidrug efflux pump operon (<jats:italic>mexCD-oprJ<\/jats:italic>) which could directly result in MDR phenotype.<\/jats:p>","DOI":"10.1556\/030.64.2017.012","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T10:01:56Z","timestamp":1496224916000},"page":"245-253","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":5,"title":["A deletion mutation in nfxB of in vitro-induced moxifloxacin-resistant Pseudomonas aeruginosa confers multidrug resistance"],"prefix":"10.1556","volume":"64","author":[{"given":"Van Chi","family":"Thai","sequence":"first","affiliation":[{"name":"1 School of Biotechnology, International University, VNU-HCMC, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang Vy","family":"Pham","sequence":"additional","affiliation":[{"name":"1 School of Biotechnology, International University, VNU-HCMC, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc Nhat Minh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"1 School of Biotechnology, International University, VNU-HCMC, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lambert","sequence":"additional","affiliation":[{"name":"2 School of Life and Health Sciences, Aston University, Birmingham, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Thu Hoai","family":"Nguyen","sequence":"additional","affiliation":[{"name":"1 School of Biotechnology, International University, VNU-HCMC, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/030.64.2017.012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/64\/3\/article-p245.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/64\/3\/article-p245.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:52:02Z","timestamp":1614599522000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/64\/3\/article-p245.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,30]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/030.64.2017.012","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,30]]}}}