{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:26:21Z","timestamp":1780964781146,"version":"3.54.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2017,11,14]]},"abstract":"<jats:p>Urinary tract infection (UTI) is a common type of infectious disease globally. The aim of this study was to detect the frequency of <jats:italic>fosA3<\/jats:italic> and <jats:italic>fosC2<\/jats:italic> genes in extended-spectrum \u03b2-lactamases (ESBL) and <jats:italic>bla<\/jats:italic><jats:sub>DHA<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CMY-2<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>CMY-42<\/jats:sub> genes in AmpC \u03b2-lactamases-producing isolates of <jats:italic>Escherichia coli<\/jats:italic>. In total, 120 isolates of <jats:italic>E. coli<\/jats:italic> were collected from three teaching hospitals between March 2014 and February 2015. Antibiotic susceptibility tests were carried out by disk diffusion method. The presence of <jats:italic>bla<\/jats:italic><jats:sub>CMY-2<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CMY-42<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>DHA<\/jats:sub>, <jats:italic>fosA3<\/jats:italic>, and <jats:italic>fosC2<\/jats:italic> genes was detected by polymerase chain reaction (PCR) and sequencing. Of the 120 strains, 92 (76.6%) were identified as ESBL producers, 30 (25%) were determined as AmpC \u03b2-lactamase producers, and 24 (20%) had both ESBL and AmpC \u03b2-lactamase enzymes. Imipenem, fosfomycin, and nitrofurantoin had the best effect against isolates of <jats:italic>E. coli<\/jats:italic>. PCR assay demonstrated that the frequency of <jats:italic>bla<\/jats:italic><jats:sub>CMY-2<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CMY-42<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>DHA<\/jats:sub> genes among AmpC \u03b2-lactamases-producing strains were 39%, 1%, and 17.5%, respectively. This study reports the first detection of fosfomycin resistance in Iran. This study indicated the increasing prevalence of UTI isolates of <jats:italic>E. coli<\/jats:italic>-harboring ESBL and AmpC \u03b2-lactamases genes in Iran. Therefore, due to the high rate of <jats:italic>bla<\/jats:italic><jats:sub>DHA<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>CMY<\/jats:sub> genes and emergence of fosfomycin-resistant <jats:italic>E. coli<\/jats:italic> isolates, we recommend continuous monitoring of antibiotic resistance as well as attention to guidelines of infection controls.<\/jats:p>","DOI":"10.1556\/030.64.2017.030","type":"journal-article","created":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T09:06:38Z","timestamp":1510736798000},"page":"15-25","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":9,"title":["Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC \u03b2-lactamases"],"prefix":"10.1556","volume":"65","author":[{"given":"Aghil","family":"Bahramian","sequence":"first","affiliation":[{"name":"1 Urology and Nephrology Research Center, Shahid Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gita","family":"Eslami","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hashemi","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Tabibi","sequence":"additional","affiliation":[{"name":"1 Urology and Nephrology Research Center, Shahid Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Heidary","sequence":"additional","affiliation":[{"name":"3 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/030.64.2017.030","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/65\/1\/article-p15.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/65\/1\/article-p15.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:45:23Z","timestamp":1614599123000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/65\/1\/article-p15.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,14]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1556\/030.64.2017.030","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,14]]}}}