{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:51:16Z","timestamp":1770972676902,"version":"3.50.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2018,2,23]]},"abstract":"<jats:p>Plasmid-mediated quinolone resistance (PMQR) determinants including, <jats:italic>qnrA, qnrB, qnrC, qnrD, qnrS, aac(6\u2032)-Ib-cr, oqxAB<\/jats:italic>, and <jats:italic>qepA<\/jats:italic>, were investigated in 214 Enterobacteriaceae strains from urine clinical samples. Antimicrobial susceptibility testing for ciprofloxacin, ceftriaxone, and imipenem was performed by broth microdilution method. All strains were screened for PMQR genes by PCR. Virulence determinants, namely <jats:italic>afa, pap, pil, sfa\/foc<\/jats:italic>, and <jats:italic>kpsMT<\/jats:italic> of eight <jats:italic>Escherichia coli<\/jats:italic> strains proven positive for at least one <jats:italic>qnr<\/jats:italic> gene, were investigated by PCR. All of the eight investigated strains carried the <jats:italic>pil<\/jats:italic> gene, showing that P fimbria is a common virulence determinant among <jats:italic>qnr<\/jats:italic> positive <jats:italic>E. coli<\/jats:italic>. Out of 214 tested strains, 38 yielded any PMQR determinant, altogether 45 genes were detected namely, 6 <jats:italic>qnrA<\/jats:italic>, 1 <jats:italic>qnrB<\/jats:italic>, 2 <jats:italic>qnrD<\/jats:italic> and 8 <jats:italic>qnrS<\/jats:italic>, 9 <jats:italic>aac(6\u2032)-Ib-cr<\/jats:italic>, and 19 <jats:italic>oqxAB<\/jats:italic>; however, neither <jats:italic>qepA<\/jats:italic> nor <jats:italic>qnrC<\/jats:italic> were detected. Notably, 18 <jats:italic>Klebsiella<\/jats:italic> spp., harbored <jats:italic>oqxAB<\/jats:italic>, nine <jats:italic>E. coli<\/jats:italic> were positive for <jats:italic>qnrS<\/jats:italic> and two <jats:italic>Morganella morganii<\/jats:italic> yielded <jats:italic>qnrD<\/jats:italic> resistance determinant. In this study, we demonstrated 17.7% prevalence of PMQR-positive Enterobacteriaceae and first reported <jats:italic>qnrD<\/jats:italic>-resistance determinant in Hungary. Altogether, 25 PMQR-positive strains were susceptible or low-level resistant to ciprofloxacin with minimum inhibitory concentration (MIC) between 0.06 and 1 mg\/L, suggesting that prevalence of PMQR determinants is underestimated and screening among clinical isolates exhibiting reduced susceptibility is necessary. Fluoroquinolone resistance breakpoints of Enterobacteriaceae were revised in 2017 by European Committee of Antimicrobial Susceptibility Testing indicating ciprofloxacin susceptibility only until 0.25 mg\/L MIC value.<\/jats:p>","DOI":"10.1556\/030.65.2018.012","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T08:26:51Z","timestamp":1519374411000},"page":"255-265","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":15,"title":["Plasmid-mediated quinolone resistance determinants in Enterobacteriaceae from urine clinical samples"],"prefix":"10.1556","volume":"65","author":[{"given":"Orsolya","family":"Szab\u00f3","sequence":"first","affiliation":[{"name":"1 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e1niel","family":"Guly\u00e1s","sequence":"additional","affiliation":[{"name":"1 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolett","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"1 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Krist\u00f3f","sequence":"additional","affiliation":[{"name":"2 Clinical Microbiology Laboratory, Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9la","family":"Kocsis","sequence":"additional","affiliation":[{"name":"1 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f3ra","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"1 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","container-title":["Acta Microbiologica et Immunologica Hungarica"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/030.65.2018.012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/65\/3\/article-p255.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/030\/65\/3\/article-p255.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T11:45:10Z","timestamp":1614599110000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/030\/65\/3\/article-p255.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,23]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1556\/030.65.2018.012","relation":{},"ISSN":["1217-8950","1588-2640"],"issn-type":[{"value":"1217-8950","type":"print"},{"value":"1588-2640","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,23]]}}}