{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T19:11:52Z","timestamp":1770837112272,"version":"3.50.1"},"reference-count":26,"publisher":"Akademiai Kiado Zrt.","issue":"4","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2018,7,14]]},"abstract":"<jats:p>The typing of non-tuberculous mycobacteria (NTM) is important from a clinical and epidemiological perspective. The polymerase chain reaction-restriction enzyme analysis (PRA) method and DNA sequence analysis method were utilized to target a gene region that codes the 65-kDa heat-shock protein for typing 150 suspected NTM samples isolated from the respiratory tract. <jats:italic>Mycobacterium abscessus, Mycobacterium xenopi, Mycobacterium fortuitum<\/jats:italic>, and <jats:italic>Mycobacterium peregrinum<\/jats:italic> were most frequently found by both methods. Six isolates that could not be defined by the PRA method were defined as <jats:italic>Nocardia cyriacigeorgica, Nocardia abscessus<\/jats:italic>, and <jats:italic>Mycobacterium intracellulare<\/jats:italic> by DNA sequence analysis. Discordance between the results of the two methods was observed for only one isolate. The isolate that was defined as <jats:italic>Mycobacterium gordonae<\/jats:italic> type 6 by the PRA method was defined as <jats:italic>Mycobacterium senegalense<\/jats:italic> by sequence analysis. The PRA method is simple and gives rapid results. Compared with DNA sequence analysis, it gives consistent and reliable results up to a ratio of 90%. DNA sequence analysis is the gold standard method in which all strains can be defined. However, given our laboratory conditions, its disadvantage is that it takes longer to reach a diagnosis than through the PRA method.<\/jats:p>","DOI":"10.1556\/030.65.2018.027","type":"journal-article","created":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T13:28:55Z","timestamp":1531747735000},"page":"515-527","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of polymerase chain reaction-restriction enzyme analysis method and DNA sequence analysis results in the identification of non-tuberculous mycobacteria"],"prefix":"10.1556","volume":"65","author":[{"given":"\u00d6zg\u00fcr","family":"Appak","sequence":"first","affiliation":[{"name":"1 Department of Medical Microbiology, Dokuz Eylul University, \u0130zmir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sel\u00e7uk","family":"T\u00fcrkel","sequence":"additional","affiliation":[{"name":"2 Department of 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