{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:42Z","timestamp":1790686842059,"version":"4.1.0"},"reference-count":44,"publisher":"Akademiai Kiado Zrt.","issue":"1","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2018,11,7]]},"abstract":"Thirty-three Pseudomonas aeruginosa<\/jats:italic> isolates, resistant to one or more \u03b2-lactams, were included in this study. Identification of tested strains was confirmed using MALDI-TOF\/MS. Phenotypic and genotypic \u03b2-lactamase patterns were investigated. Most of the isolates were resistant to carbapenems (32 out of 33) and to the extended-spectrum cephalosporins (ESC) (30 out of 33). Phenotypically, the production of extended-spectrum beta-lactamase (ESBL), metallo-\u03b2-lactamases (MBL), and carbapenemases was detected in 10, 23, and 9 isolates, respectively. However, AmpC hyperproduction was not phenotypically detected among all isolates. Genotypically, ESBL and MBL encoding genes were detected in 23 and 27 isolates, respectively. Altogether 27 strains were detected as bla<\/jats:italic>VIM<\/jats:sub> positive and 16 strains carried bla<\/jats:italic>OXA-10<\/jats:sub> gene. To the best of our knowledge, this is the first report of P. aeruginosa<\/jats:italic> clinical isolates harboring bla<\/jats:italic>VEB<\/jats:sub> together with bla<\/jats:italic>GES<\/jats:sub> in Egypt, where 5 of our 30 ESC-resistant isolates showed this genotype. Our results confirmed that resistance of P. aeruginosa<\/jats:italic> isolates to \u03b2-lactam antibiotics is mediated via multiple \u03b2-lactamases belonging to different molecular classes. To the best of our knowledge, this is the first report of bla<\/jats:italic>VEB<\/jats:sub> among P. aeruginosa<\/jats:italic> clinical isolates from Egypt. Ten isolates harbored bla<\/jats:italic>VEB<\/jats:sub> and five of them co-harbored bla<\/jats:italic>VEB<\/jats:sub> together with bla<\/jats:italic>GES<\/jats:sub>, bla<\/jats:italic>VIM<\/jats:sub>, and bla<\/jats:italic>OXA-10.<\/jats:sub><\/jats:p>","DOI":"10.1556\/030.65.2018.044","type":"journal-article","created":{"date-parts":[[2018,11,7]],"date-time":"2018-11-07T14:35:46Z","timestamp":1541601346000},"page":"131-142","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":12,"title":["Emergence of blaVEB and blaGES among VIM-producing Pseudomonas aeruginosa clinical isolates in Alexandria, Egypt"],"prefix":"10.1556","volume":"66","author":[{"given":"Ahmed","family":"Gaballah","sequence":"first","affiliation":[{"name":"1 Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Elbaradei","sequence":"additional","affiliation":[{"name":"2 Faculty of Pharmacy and Drug Manufacturing, Department of Microbiology and Immunology, Pharos University in Alexandria, Alexandria, Egypt"},{"name":"3 Alexandria University Hospital, Alexandria University, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel","family":"Elsheredy","sequence":"additional","affiliation":[{"name":"1 Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola","family":"Kader","sequence":"additional","affiliation":[{"name":"1 Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, 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