{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T15:51:19Z","timestamp":1785167479318,"version":"3.55.0"},"reference-count":112,"publisher":"Akademiai Kiado Zrt.","issue":"2","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2018,11,22]]},"abstract":"<jats:p>Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them,<jats:italic>Enterococcus faecalis<\/jats:italic>is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53<jats:italic>E. faecalis<\/jats:italic>isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible to at least one agent in \u22653 antimicrobial categories were defined as multidrug-resistant (MDR). Detection of antimicrobial resistance genes was performed using standard procedures. According to MDR definition, all of the isolates were MDR (100%). High-level gentamicin resistance was observed among 50.9% of them (MIC\u2009\u2265\u2009500\u2009\u03bcg\/ml). The distributions of<jats:italic>aac(6\u2032)-Ie-aph(2\u2032\u2032)-Ia<\/jats:italic>and<jats:italic>aph(3\u2032)-IIIa<\/jats:italic>genes were 47.2% and 69.8%, respectively. The<jats:italic>aph(2\u2032\u2032)-Ib, aph(2\u2032\u2032)-Ic, aph(2\u2032\u2032)-Id<\/jats:italic>, and<jats:italic>ant(4\u2032)-Ia<\/jats:italic>genes were not detected. Vancomycin resistance was found in 45.3% of strains. The<jats:italic>vanA<\/jats:italic>gene was detected in 37.7% of isolates, whereas<jats:italic>vanB<\/jats:italic>and<jats:italic>vanC<jats:sub>1<\/jats:sub><\/jats:italic>genes were not observed in any strain. Erythromycin resistance rate was 79.2% and the frequencies of<jats:italic>ermB<\/jats:italic>and<jats:italic>ermC<\/jats:italic>genes were 88.6% and 69.8%, respectively. The<jats:italic>ermA<\/jats:italic>and<jats:italic>msrA<\/jats:italic>genes were not present in any of the isolates. Our data indicate a high rate of MDR<jats:italic>E. faecalis<\/jats:italic>strains. All of high-level gentamicin-resistant isolates carried at least one of<jats:italic>aac(6\u2032)-Ie-aph(2\u2032\u2032)-Ia<\/jats:italic>or<jats:italic>aph(3\u2032)-IIIa<\/jats:italic>genes. Distribution of<jats:italic>vanA<\/jats:italic>was notable among the isolates. In addition,<jats:italic>ermB<\/jats:italic>and<jats:italic>ermC<\/jats:italic>were accountable for resistance to erythromycin.<\/jats:p>","DOI":"10.1556\/030.65.2018.046","type":"journal-article","created":{"date-parts":[[2018,11,22]],"date-time":"2018-11-22T11:04:55Z","timestamp":1542884695000},"page":"203-217","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":15,"title":["High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates"],"prefix":"10.1556","volume":"66","author":[{"given":"Asieh","family":"Taji","sequence":"first","affiliation":[{"name":"1 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Heidari","sequence":"additional","affiliation":[{"name":"1 Department of Bacteriology and Virology, School of Medicine, Shiraz 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