{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:03:21Z","timestamp":1790420601942,"version":"4.1.0"},"reference-count":48,"publisher":"Akademiai Kiado Zrt.","issue":"3","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["AMicr"],"published-print":{"date-parts":[[2019,2,21]]},"abstract":"<jats:p>The emergence of 16S rRNA methylase genes encoded on plasmids confers high-level aminoglycoside resistance (HLAR). This study aimed to investigate the prevalence of 16S rRNA methylases among <jats:italic>Enterobacter cloacae<\/jats:italic> strains isolated from an Ahvaz teaching hospital, Iran. A total of 68 <jats:italic>E. cloacae<\/jats:italic> clinical strains were collected between November 2017 and September 2018. The MICs of aminoglycosides were assessed using the agar dilution method. The presence of 16S rRNA methylase genes, including <jats:italic>armA, rmtA<\/jats:italic> to <jats:italic>rmtH<\/jats:italic>, and <jats:italic>nmpA<\/jats:italic> was evaluated by PCR. The transferability of 16S rRNA methylase-harboring plasmids was evaluated by conjugation assay. The genetic diversity of all isolates was evaluated by ERIC-PCR. The <jats:italic>armA<\/jats:italic> and <jats:italic>rmtB<\/jats:italic> genes were the only 16S rRNA methylase genes detected in this study (29 out of 68 isolates; 42.64%). The transferability by conjugation was observed in 23 <jats:italic>rmtB<\/jats:italic> or\/and <jats:italic>armA<\/jats:italic> positive donors. HLAR phenotype was in 33 of 68 strains. Ten clonal types were obtained by ERIC-PCR and significant associations (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) were between the clone types and aminoglycoside susceptibility, as well as with profile of the 16S rRNA methylase genes. In conclusion, both horizontal transfer and clonal spread are responsible for dissemination of the <jats:italic>rmtB<\/jats:italic> and <jats:italic>armA<\/jats:italic> genes among <jats:italic>E. cloacae<\/jats:italic> strains.<\/jats:p>","DOI":"10.1556\/030.66.2019.009","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T08:38:31Z","timestamp":1550738311000},"page":"337-348","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.1","source":"Crossref","is-referenced-by-count":6,"title":["High distribution of 16S rRNA methylase genes rmtB and armA among Enterobacter cloacae strains isolated from an Ahvaz teaching hospital, Iran"],"prefix":"10.1556","volume":"66","author":[{"given":"Mansour","family":"Amin","sequence":"first","affiliation":[{"name":"1 Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"},{"name":"2 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Golshan","family":"Mehdipour","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahereh","family":"Navidifar","sequence":"additional","affiliation":[{"name":"2 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref121","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.ijantimicag.2018.03.016","article-title":"High prevalence of 16S rRNA methyltransferases among carbapenemase-producing Enterobacteriaceae in the UK and Ireland","volume":"52","author":"Taylor","year":"2018","journal-title":"Int J Antimicrob 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