{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T05:29:09Z","timestamp":1761197349577},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"4","content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["Acta Physiologica Hungarica"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>This study was carried out to evaluate the effects of noscapine, a benzylisoquinoline alkaloid from opium poppy, on oligodendrocyte during ischemia\/reperfusion-induced excitotoxic injury. Changes in intracellular calcium levels due to chemical ischemia and nitric oxide (NO) production during ischemia\/reperfusion were evaluated as the hallmarks of ischemia-derived excitotoxic event. OLN-93 cell line (a permanent immature rat oligodendrocyte) was used as a model of oligodendrocyte. 30- or 60-minute-oxygen\u2014glucose deprivation\/24 hours reperfusion were used to induce excitotoxicity. MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay was used to evaluate cell viability. Ratiometric fluorescence microscopy using Ca<jats:sup>2+<\/jats:sup>-sensitive indicator Fura-2\/AM was utilized to assess intracellular calcium levels. NO production was evaluated by Griess method. Noscapine (4 \u03bcM) significantly attenuated intracellular Ca<jats:sup>2+<\/jats:sup> elevation (<jats:italic>P<\/jats:italic> &lt; 0.001). Also, noscapine significantly decreased NO production during a 30-minute oxygen\u2014glucose deprivation\/reperfusion (<jats:italic>P<\/jats:italic> &lt; 0.01). The inhibitory effect of noscapine (4 \u03bcM) on intracellular Ca<jats:sup>2+<\/jats:sup> was greater than ionotropic glutamate receptors antagonists. Noscapine is protective against ischemia\/reperfusion-induced excitotoxic injury in OLN-93 oligodendrocyte. This protective effect seems to be related to attenuation of intracellular Ca<jats:sup>2+<\/jats:sup> overload and NO production.<\/jats:p>","DOI":"10.1556\/036.102.2015.4.2","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T18:50:01Z","timestamp":1450723801000},"page":"351-362","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":7,"title":["Noscapine protects OLN-93 oligodendrocytes from ischemia-reperfusion damage: Calcium and nitric oxide involvement"],"prefix":"10.1556","volume":"102","author":[{"given":"S","family":"Nadjafi","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S-A","family":"Ebrahimi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Rahbar-Roshandel","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","container-title":["Acta Physiologica Hungarica"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/036\/102\/4\/article-p351.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/036\/102\/4\/article-p351.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/036\/102\/4\/article-p351.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T15:50:51Z","timestamp":1704297051000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/036\/102\/4\/article-p351.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/036.102.2015.4.2","relation":{},"ISSN":["0231-424X","1588-2683"],"issn-type":[{"value":"0231-424X","type":"print"},{"value":"1588-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12]]}}}