{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T01:58:43Z","timestamp":1762999123128},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"4","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>\n          <jats:italic>HSP90B1<\/jats:italic> is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. <jats:italic>Toxoplasma gondii<\/jats:italic> causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of <jats:italic>Gallus domesticus<\/jats:italic> embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with <jats:italic>HSP90B1<\/jats:italic> gene expression. Embryos\u2019 eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the <jats:italic>HSP90B1<\/jats:italic> gene upon experimental infection. This is a promising model to better understand <jats:italic>T. gondii<\/jats:italic> etiopathogeny.<\/jats:p>","DOI":"10.1556\/1886.2015.00024","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T07:09:53Z","timestamp":1447398593000},"page":"316-320","update-policy":"http:\/\/dx.doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":3,"title":["Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: A promising ocular toxoplasmosis model"],"prefix":"10.1556","volume":"5","author":[{"given":"Alex M.","family":"Nasar\u00e9","sequence":"first","affiliation":[{"name":"Morphology and Genetics Department, Universidade Federal de S\u00e3o Paulo, Botucatu St., 740, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto C.","family":"Tedesco","sequence":"additional","affiliation":[{"name":"Morphology and Genetics Department, Universidade Federal de S\u00e3o Paulo, Botucatu St., 740, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila C.","family":"Cristovam","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, Instituto de Medicina Tropical de S\u00e3o Paulo, Botucatu st., 822, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos A.","family":"Cenedese","sequence":"additional","affiliation":[{"name":"Nephrology Discipline, Universidade Federal de S\u00e3o Paulo, Botucatu St., 740, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s J.","family":"Galisteo Jr.","sequence":"additional","affiliation":[{"name":"Protozoology Department, Instituto de Medicina Tropical de S\u00e3o Paulo, Dr. En\u00e9as de Carvalho Aguiar Av., 470, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heitor F.","family":"Andrade Jr.","sequence":"additional","affiliation":[{"name":"Protozoology Department, Instituto de Medicina Tropical de S\u00e3o Paulo, Dr. En\u00e9as de Carvalho Aguiar Av., 470, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 \u00c1lvaro P.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, Instituto de Medicina Tropical de S\u00e3o Paulo, Botucatu st., 822, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9rik V.","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Structural biology laboratory, Instituto Oswaldo Cruz, Brasil Av, 4365, Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helene S.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Structural biology laboratory, Instituto Oswaldo Cruz, Brasil Av, 4365, Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis G.","family":"Alonso","sequence":"additional","affiliation":[{"name":"Morphology and Genetics Department, Universidade Federal de S\u00e3o Paulo, Botucatu St., 740, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/5\/4\/article-p316.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/5\/4\/article-p316.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/5\/4\/article-p316.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:32:36Z","timestamp":1704299556000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/5\/4\/article-p316.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/1886.2015.00024","relation":{},"ISSN":["2062-509X"],"issn-type":[{"value":"2062-509X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]}}}