{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T04:14:32Z","timestamp":1775276072139,"version":"3.50.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"4","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>ESBL (extended-spectrum-\u03b2-lactamase)-positive Enterobacteriaceae, which colonized European soldiers in tropical Western African Mali, were subjected to a molecular assessment of their resistance determinants. By doing so, a better insight into the locally endemic pattern of ESBL-associated \u03b2-lactamase genes was aspired.<\/jats:p>\n        <jats:p>From a previous study on diarrhea in European soldiers on deployment in tropical Mali, 15 ESBL-positive <jats:italic>Escherichia coli<\/jats:italic> with demonstrated high clonal diversity and one positive <jats:italic>Klebsiella pneumoniae<\/jats:italic> were assessed. Polymerase chain reactions (PCRs) for <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>TEM<\/jats:italic><\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>SHV<\/jats:italic><\/jats:sub> \u03b2-lactamase genes with subsequent sequencing for the discrimination of ESBL- and non-ESBL variants were performed, followed by four group-specific PCRs for <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CTX-M<\/jats:italic><\/jats:sub> genes.<\/jats:p>\n        <jats:p>Non-ESBL-associated <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>TEM-1<\/jats:italic><\/jats:sub> was identified in six out of 15 (40%) E. coli strains, while 100% of the assessed strains were positive for group I <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CTX-M<\/jats:italic><\/jats:sub>.<\/jats:p>\n        <jats:p>Considering the known clonal diversity of the assessed strains, the striking restriction to one group of <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CTX-M<\/jats:italic><\/jats:sub> genes accounting for the ESBL phenotypes of the isolates suggests little genetic exchange in the local setting. Under such circumstances of restricted numbers of locally endemic target genes, PCR-based screening approaches for ESBL colonization might be promising.<\/jats:p>","DOI":"10.1556\/1886.2015.00037","type":"journal-article","created":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T06:33:59Z","timestamp":1445495639000},"page":"281-284","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":10,"title":["\u03b2-lactamases encoded by blaCTX-M group I genes as determinants of resistance of ESBL-positive enterobacteriaceae in European soldiers in tropical Mali"],"prefix":"10.1556","volume":"5","author":[{"given":"Ralf Matthias","family":"Hagen","sequence":"first","affiliation":[{"name":"Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hagen","family":"Hinz","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Berlin, Germany"},{"name":"Institute for Microbiology and Immunology, University Medicine Rostock, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/5\/4\/article-p281.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/5\/4\/article-p281.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/5\/4\/article-p281.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:32:24Z","timestamp":1704299544000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/5\/4\/article-p281.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/1886.2015.00037","relation":{},"ISSN":["2062-509X"],"issn-type":[{"value":"2062-509X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]}}}