{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:53:13Z","timestamp":1790506393807,"version":"4.1.0"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"4","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>\n          <jats:italic>Borrelia burgdorferi<\/jats:italic>, the causative agent of Lyme disease, is capable of forming biofilm in vivo and in vitro, a structure well known for its resistance to antimicrobial agents. For the formation of biofilm, signaling processes are required to communicate with the surrounding environment such as it was shown for the RpoN\u2014RpoS alternative sigma factor and for the LuxS quorum-sensing pathways. Therefore, in this study, the wild-type <jats:italic>B. burgdorferi<\/jats:italic> and different mutant strains lacking RpoN, RpoS, and LuxS genes were studied for their growth characteristic and development of biofilm structures and markers as well as for their antibiotic sensitivity. Our results showed that all three mutants formed small, loosely formed aggregates, which expressed previously identified <jats:italic>Borrelia<\/jats:italic> biofilm markers such as alginate, extracellular DNA, and calcium. All three mutants had significantly different sensitivity to doxycyline in the early log phase spirochete cultures; however, in the biofilm rich stationary cultures, only LuxS mutant showed increased sensitivity to doxycyline compared to the wild-type strain. Our findings indicate that all three mutants have some effect on <jats:italic>Borrelia<\/jats:italic> biofilm, but the most dramatic effect was found with LuxS mutant, suggesting that the quorum-sensing pathway plays an important role of <jats:italic>Borrelia<\/jats:italic> biofilm formation and antibiotic sensitivity.<\/jats:p>","DOI":"10.1556\/1886.2016.00026","type":"journal-article","created":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T13:25:16Z","timestamp":1478179516000},"page":"272-286","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":30,"title":["Effect of RpoN, RpoS and LuxS pathways on the biofilm formation and antibiotIc sensitivity of Borrelia burgdorferi"],"prefix":"10.1556","volume":"6","author":[{"given":"Eva","family":"Sapi","sequence":"first","affiliation":[{"name":"Department of Biology and Environmental Science, University of New Haven, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka A. S.","family":"Theophilus","sequence":"additional","affiliation":[{"name":"Department of Biology and Environmental Science, University of New Haven, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Truc V.","family":"Pham","sequence":"additional","affiliation":[{"name":"Department of Biology and Environmental Science, University of New Haven, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Burugu","sequence":"additional","affiliation":[{"name":"Department of Biology and Environmental Science, University of New Haven, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David F.","family":"Luecke","sequence":"additional","affiliation":[{"name":"Department of Biology and Environmental Science, University of New Haven, West Haven, CT 06516, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/6\/4\/article-p272.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/6\/4\/article-p272.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/6\/4\/article-p272.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:33:50Z","timestamp":1704299630000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/6\/4\/article-p272.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/1886.2016.00026","relation":{},"ISSN":["2062-509X"],"issn-type":[{"value":"2062-509X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,12]]}}}