{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T09:46:55Z","timestamp":1771667215030,"version":"3.50.1"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"1","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>Reliable identification of pathogenic <jats:italic>Burkholderia<\/jats:italic> spp. like <jats:italic>Burkholderia mallei<\/jats:italic> and <jats:italic>Burkholderia pseudomallei<\/jats:italic> in clinical samples is desirable. Three different selective media were assessed for reliability and selectivity with various <jats:italic>Burkholderia<\/jats:italic> spp. and nontarget organisms.<\/jats:p>\n        <jats:p>\n          <jats:italic>Mast Burkholderia<\/jats:italic> cepacia agar, Ashdown + gentamicin agar, and <jats:italic>B. pseudomallei<\/jats:italic> selective agar were compared. A panel of 116 reference strains and well-characterized clinical isolates, comprising 30 <jats:italic>B. pseudomallei<\/jats:italic>, 20 <jats:italic>B. mallei<\/jats:italic>, 18 other <jats:italic>Burkholderia<\/jats:italic> spp., and 48 nontarget organisms, was used for this assessment.<\/jats:p>\n        <jats:p>While all <jats:italic>B. pseudomallei<\/jats:italic> strains grew on all three tested selective agars, the other <jats:italic>Burkholderia<\/jats:italic> spp. showed a diverse growth pattern. Nontarget organisms, i.e., nonfermentative rod-shaped bacteria, other species, and yeasts, grew on all selective agars. Colony morphology did not allow unambiguous discrimination.<\/jats:p>\n        <jats:p>While the assessed selective media reliably allowed the growth of a wide range of <jats:italic>B. pseudomallei<\/jats:italic> strains, growth of other <jats:italic>Burkholderia<\/jats:italic> spp. is only partially ensured. Growth of various nontarget organisms has to be considered. Therefore, the assessed media can only be used in combination with other confirmative tests in the diagnostic procedure for the screening for melioidosis or glanders.<\/jats:p>","DOI":"10.1556\/1886.2016.00037","type":"journal-article","created":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T02:01:29Z","timestamp":1484013689000},"page":"15-36","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of mast Burkholderia cepacia, ashdown + gentamicin, and Burkholderia pseudomallei selective agar for the selective growth of Burkholderia spp."],"prefix":"10.1556","volume":"7","author":[{"given":"Carola","family":"Edler","sequence":"first","affiliation":[{"name":"Department of Dermatology, German Armed Forces Hospital of Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri","family":"Derschum","sequence":"additional","affiliation":[{"name":"CBRN Defence, Safety and Environmental Protection School, Science Division"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirko","family":"K\u00f6hler","sequence":"additional","affiliation":[{"name":"Bundeswehr Medical Academy, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Neubauer","sequence":"additional","affiliation":[{"name":"Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany"},{"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf Matthias","family":"Hagen","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, Bundeswehr Medical Academy, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"425","container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/7\/1\/article-p15.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/7\/1\/article-p15.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/7\/1\/article-p15.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:34:12Z","timestamp":1704299652000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/7\/1\/article-p15.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1556\/1886.2016.00037","relation":{},"ISSN":["2062-509X"],"issn-type":[{"value":"2062-509X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,3]]}}}