{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:17:55Z","timestamp":1790248675073,"version":"4.1.0"},"reference-count":0,"publisher":"Akademiai Kiado Zrt.","issue":"2","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Bacterial antimicrobial resistance mediated by the production of extended-spectrum \u03b2-lactamases (ESBLs) is considered a major threat for treatment of <jats:italic>Salmonella<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> infections. This study aimed to investigate antibiotic resistance patterns of <jats:italic>Salmonella<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> spp. and presence of CTX-M from three teaching hospitals in Iran. In the present study, 58 clinical <jats:italic>Shigella<\/jats:italic> and 91 <jats:italic>Salmonella<\/jats:italic> isolates were recovered between 2009 and 2013 from 3 teaching hospitals in Iran. After culture and antimicrobial susceptibility testing, ESBL-positive isolates were subjected to further investigations. These included polymerase chain reaction (PCR) amplification and DNA sequencing of <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> encoding plasmid. In both genera, high sensitivity to gentamicin and amikacin, but high resistance to ampicillin, tetracycline, and trimethoprim\u2014sulfamethoxazole, was found. Molecular investigation showed that 31.8% isolates of <jats:italic>Salmonella<\/jats:italic> spp. and 34.48% isolates of <jats:italic>Shigella<\/jats:italic> spp. were CTX-M positive and all of them were also positive for ISEcpI. Protein translation, comparing with reference sequences, showed that all CTX-M isolates belong to CTX-M-15. The present study suggests that the resistance of ESBLs-producing <jats:italic>Salmonella<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> spp. in Iran hospitals is very serious. Therefore, strategies to minimize the spread of ESBL-producing isolates should be implemented.<\/jats:p>","DOI":"10.1556\/1886.2017.00004","type":"journal-article","created":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T09:56:23Z","timestamp":1493200583000},"page":"133-137","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":15,"title":["The prevalence of CTX-M-15 extended-spectrum \u03b2-lactamases among Salmonella spp. and Shigella spp. isolated from three Iranian hospitals"],"prefix":"10.1556","volume":"7","author":[{"given":"Abed Zahedi","family":"Bialvaei","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tala","family":"Pourlak","sequence":"additional","affiliation":[{"name":"Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Aghamali","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Asgharzadeh","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pourya","family":"Gholizadeh","sequence":"additional","affiliation":[{"name":"Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein Samadi","family":"Kafil","sequence":"additional","affiliation":[{"name":"Drug Applied Research Center, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/7\/2\/article-p133.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/7\/2\/article-p133.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/7\/2\/article-p133.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:34:38Z","timestamp":1704299678000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/7\/2\/article-p133.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1556\/1886.2017.00004","relation":{},"ISSN":["2062-509X"],"issn-type":[{"value":"2062-509X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6]]}}}