{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:38:28Z","timestamp":1791887908252,"version":"4.3.4"},"reference-count":22,"publisher":"Akademiai Kiado Zrt.","issue":"1","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2018,3]]},"abstract":"\u03b2-Lactam antibiotics are widely used to treat urinary tract infections in Nigeria. This study aimed to determine the presence and characteristics of extended spectrum \u03b2-lactamases in commonly isolated uropathogenic Gram-negative bacteria (GNB) in Nigeria.<\/jats:p>\n Fifty non-duplicate GNB isolates consisting of Escherichia coli<\/jats:italic>, 19; Klebsiella pneumoniae<\/jats:italic>, 21; and Pseudomonas aeruginosa<\/jats:italic>, 10 were obtained from three tertiary hospitals in Nigeria. The antibiotic susceptibility testing of all isolates to a panel of antibiotics including minimum inhibitory concentrations (MICs) and extended spectrum \u03b2-lactamases was determined. Polymerase chain reactions and sequencing were used to detect \u03b2-lactam genes.<\/jats:p>\n Polymerase chain reactions and sequencing identified varying extended spectrum \u03b2-lactamases (ESBLs) encoding genes for 24 isolates (48.0%). Cefotaximase-Munich (CTX-M) 15 was the dominant gene with 20\/24 of the isolates positive at 83.3%; multiple genes (2 to 6 ESBL genes) were found in 20 of the isolates. The isolates encoded other genes such as CTX-M-14, 33.3%; sulfhydryl variable (SHV) variants, 58.3%; oxacillinase (OXA) variants, 70.8%; OXA-10, 29.2%; and Vietnamese extended \u03b2-lactamase (VEB) 1, 25.0%. There was no difference between the MIC50<\/jats:sub> and MIC90<\/jats:sub> of all the isolates.<\/jats:p>\n The high-level multidrug resistance of uropathogens to third generation cephalosporins including other antibiotics used in this study is strongly associated with carriage of ESBLs, predominantly CTX-M-15, as well as CTX-X-M-14, OXA-10, and VEB-1.<\/jats:p>","DOI":"10.1556\/1886.2017.00012","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T08:32:07Z","timestamp":1520411527000},"page":"20-24","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":41,"title":["CTX-M-15 is established in most multidrug-resistant uropathogenic Enterobacteriaceae and Pseudomonaceae from hospitals in Nigeria"],"prefix":"10.1556","volume":"8","author":[{"given":"David Olusoga","family":"Ogbolu","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria"},{"name":"Antimicrobials Research Group, Immunity and Infection, Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. A.","family":"Terry Alli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark Alexander","family":"Webber","sequence":"additional","affiliation":[{"name":"Antimicrobials Research Group, Immunity and Infection, Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeolu Sunday","family":"Oluremi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omoboriowo Moses","family":"Oloyede","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Osogbo Campus, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref1","series-title":"Crit Care Res Pract","first-page":"625170","article-title":"ESBL: A clear and present danger?","volume":"2012","author":"Dhillon\n RHP","year":"2012","unstructured":"Dhillon\n RHP\n \n \n Clark\n J\n \n . ESBL: A clear and present danger?Crit Care Res Pract. 2012;2012:625170."},{"key":"ref2","series-title":"Ann Rev Microbiol","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1146\/annurev-micro-090110-102911","article-title":"Epidemiological expansion, structural studies and clinical challenges of new beta-lactamases from gram-negative bacteria","volume":"65","author":"Bush\n K","year":"2011","unstructured":"Bush\n K\n \n \n Fisher\n JF\n \n . Epidemiological expansion, structural studies and clinical challenges of new beta-lactamases from gram-negative bacteria. Ann Rev Microbiol. 2011;65:455\u201378.10.1146\/annurev-micro-090110-102911"},{"key":"ref3","series-title":"Lancet Infect Dis","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S1473-3099(08)70041-0","article-title":"Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern","volume":"8","author":"Pitout\n JD","year":"2008","unstructured":"Pitout\n JD\n \n \n Laupland\n KB\n \n . Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis. 2008;8:159\u201366.10.1016\/S1473-3099(08)70041-0"},{"key":"ref4","series-title":"Antimicrob Agents Chemother","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1128\/AAC.01707-08","article-title":"Resistance plasmid families in Enterobacteriaceae","volume":"53","author":"Carattoli\n A","year":"2009","unstructured":"Carattoli\n A\n \n . Resistance plasmid families in Enterobacteriaceae. Antimicrob Agents Chemother. 2009;53:2227\u201338.10.1128\/AAC.01707-08"},{"key":"ref5","series-title":"Curr Opin Investig Drugs","first-page":"172","article-title":"Addressing the challenge of extended-spectrum beta-lactamases","volume":"10","author":"Zahar\n JR","year":"2009","unstructured":"Zahar\n JR\n \n \n Lortholary\n O\n \n \n Martin\n C\n \n \n Potel\n G\n \n \n Plesiat\n P\n \n \n Nordmann\n P\n \n . Addressing the challenge of extended-spectrum beta-lactamases. Curr Opin Investig Drugs. 2009;10:172\u201380."},{"key":"ref6","series-title":"Int J Antimicrob Agents","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1016\/j.ijantimicag.2014.01.014","article-title":"High-level and novel mechanisms of carbapenem resistance in Gram-negative bacteria from tertiary hospitals in Nigeria","volume":"43","author":"Ogbolu\n DO","year":"2014","unstructured":"Ogbolu\n DO\n \n \n Webber\n MA\n \n . 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J Antimicrob Chemother. 2001;48;5\u201316.10.1093\/jac\/48.suppl_1.5"},{"key":"ref9","series-title":"Antimicrob Agents Chemother","doi-asserted-by":"publisher","first-page":"3214","DOI":"10.1128\/AAC.47.10.3214-3221.2003","article-title":"Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149:K91 isolates obtained over a 23-year period from pigs","volume":"47","author":"Maynard\n C","year":"2003","unstructured":"Maynard\n C\n \n \n Fairbrother\n JM\n \n \n Bekal\n S\n \n \n Sanschagrin\n F\n \n \n Levesque\n RC\n \n \n Brousseau\n R\n \n \n \n Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149:K91 isolates obtained over a 23-year period from pigs. 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High level and novel mechanisms of carbapenem resistance in Gram negative bacteria from tertiary hospitals in Nigeria. Int J Antimicrob Agents. 2014;43:412\u20137.10.1016\/j.ijantimicag.2014.01.014"},{"key":"ref14","series-title":"J Infect Dev Ctries","doi-asserted-by":"publisher","first-page":"850","DOI":"10.3855\/jidc.1490","article-title":"Emergence of extended-spectrum beta-lactamase-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco","volume":"5","author":"Bourjilat\n F","year":"2011","unstructured":"Bourjilat\n F\n \n \n Bouchrif\n B\n \n \n Dersi\n N\n \n \n Claude\n JDPG\n \n \n Amarouch\n H\n \n \n Timinouni\n M\n \n . Emergence of extended-spectrum beta-lactamase-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco. 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Antimicrob Agents Chemother. 2012;39:350\u20135.10.1128\/AAC.39.2.350"},{"key":"ref22","series-title":"Antimicrob Agents Chemother","doi-asserted-by":"publisher","first-page":"4178","DOI":"10.1128\/AAC.01301-09","article-title":"An ertapenem-resistant extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae clone carries a novel OmpK36 porin variant","volume":"54","author":"Garcia-Fernandez\n A","year":"2010","unstructured":"Garcia-Fernandez\n A\n \n \n Miriagou\n V\n \n \n Papagiannitsis\n CC\n \n \n Giordano\n A\n \n \n Venditti\n M\n \n \n Mancini\n C\n \n \n Carattoli\n A\n \n . An ertapenem-resistant extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae clone carries a novel OmpK36 porin variant. Antimicrob Agents Chemother2010;54:4178\u201384.10.1128\/AAC.01301-09"}],"container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"http:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/1886.2017.00012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/8\/1\/article-p20.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/8\/1\/article-p20.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/8\/1\/article-p20.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:35:58Z","timestamp":1704299758000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/8\/1\/article-p20.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3]]},"references-count":22,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1556\/1886.2017.00012","relation":{},"ISSN":["2062-8633"],"issn-type":[{"value":"2062-8633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3]]}}}