{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:35:06Z","timestamp":1790613306480,"version":"4.1.0"},"reference-count":65,"publisher":"Akademiai Kiado Zrt.","issue":"4","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["akjournals.com"],"crossmark-restriction":true},"short-container-title":["EuJMI"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>\n          <jats:italic>Background<\/jats:italic>: The objective of this study was to assess an in-house loop-mediated isothermal amplification (LAMP) platform for malaria parasite detection and identification on species level.<\/jats:p>\n        <jats:p>\n          <jats:italic>Methods<\/jats:italic>: LAMP primers specific for the human <jats:italic>Plasmodium<\/jats:italic> spp., namely, <jats:italic>P. falciparum<\/jats:italic>, <jats:italic>P. vivax<\/jats:italic>, <jats:italic>P. ovale<\/jats:italic>, <jats:italic>P. malariae<\/jats:italic>, and <jats:italic>P. knowlesi<\/jats:italic>, as well as genus-specific primers, were tested against a composite gold standard comprising microscopy from thick and thin blood films, commercial genus-specific Meridian <jats:italic>illumigene<\/jats:italic> Malaria LAMP, in-house real-time polymerase chain reaction (PCR), and commercial fast-track diagnostics (FTD) Malaria differentiation PCR.<\/jats:p>\n        <jats:p>\n          <jats:italic>Results<\/jats:italic>: Of the 523 blood samples analyzed, the composite gold standard indicated 243 <jats:italic>Plasmodium<\/jats:italic>-species-DNA-containing samples (46.5%). Sensitivity and specificity of the analyzed genus- and species-specific LAMP primers were 71.0%\u2013100.0% and 90.8%\u2013100.0%, respectively. The influence of parasitemia was best documented for <jats:italic>P. falciparum<\/jats:italic>-specific LAMP with sensitivity values of 35.5% (22\/62) for microscopically negative samples containing <jats:italic>P. falciparum<\/jats:italic> DNA, 50% (19\/38) for parasitemia \u226450\/\u03bcL, 84% (21\/25) for parasitemia \u2264500\/\u03bcL, and 100% (92\/92) for parasitemia &gt;500\/\u03bcL.<\/jats:p>\n        <jats:p>\n          <jats:italic>Conclusions<\/jats:italic>: In our hands, performance characteristics of species-specific in-house LAMP for malaria lack reliability required for diagnostic laboratories. The use of the easy-to-apply technique for surveillance purposes may be considered.<\/jats:p>","DOI":"10.1556\/1886.2018.00020","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T12:16:20Z","timestamp":1538741780000},"page":"112-118","update-policy":"https:\/\/doi.org\/10.1556\/policypage.crossmark.crossref.2","source":"Crossref","is-referenced-by-count":10,"title":["Poor diagnostic performance of a species-specific loop-mediated isothermal amplification (LAMP) platform for malaria"],"prefix":"10.1556","volume":"8","author":[{"given":"Hans","family":"Kollenda","sequence":"first","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Tropical Microbiology and Entomology Unit, Bundeswehr Hospital Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf Matthias","family":"Hagen","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, Bundeswehr Medical Academy, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Hanke","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Rojak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Tropical Microbiology and Entomology Unit, Bundeswehr Hospital Hamburg, Hamburg, Germany"},{"name":"Department of Infectious Diseases and Tropical Medicine, Bundeswehr Hospital Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Hinz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Tropical Microbiology and Entomology Unit, Bundeswehr Hospital Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Wassill","sequence":"additional","affiliation":[{"name":"Amplex Diagnostics GmbH, Gars-Bahnhof, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Poppert","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Basel, Switzerland"},{"name":"Faculty of Medicine, University Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egbert","family":"Tannich","sequence":"additional","affiliation":[{"name":"National Reference Centre for Tropical Pathogens, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagen","family":"Frickmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene, Tropical Microbiology and Entomology Unit, Bundeswehr Hospital Hamburg, Hamburg, Germany"},{"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"425","reference":[{"key":"ref1","series-title":"Clin Chem","first-page":"303","article-title":"Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification","volume":"52","author":"Poon\n              LL","year":"2006","unstructured":"Poon\n              LL\n            , WongBW, MaEH, ChanKH, ChowLM, AbeyewickremeW, et al\n          Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006;52:303\u20136.16339303"},{"key":"ref2","series-title":"Acta Trop","first-page":"61","article-title":"Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)","volume":"113","author":"Chen\n              JH","year":"2010","unstructured":"Chen\n              JH\n            , LuF, LimCS, KimJY, AhnHJ, SuhIB, et al\n          Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP). Acta Trop. 2010;113:61\u20135.19778519"},{"key":"ref3","series-title":"Parasitol Int","first-page":"414","article-title":"Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for clinical detection of Plasmodium falciparum gametocytes","volume":"59","author":"Buates\n              S","year":"2010","unstructured":"Buates\n              S\n            , BantuchaiS, SattabongkotJ, HanET, TsuboiT, UdomsangpetchR, et al\n          Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for clinical detection of Plasmodium falciparum gametocytes. Parasitol Int. 2010;59:414\u201320.20542143"},{"key":"ref4","series-title":"PLoS One","first-page":"e13733","article-title":"Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria","volume":"5","author":"Lucchi\n              NW","year":"2010","unstructured":"Lucchi\n              NW\n            , DemasA, NarayananJ, SumariD, KabanywanyiA, KachurSP, et al\n          Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria. PLoS One. 2010;5:e13733."},{"key":"ref5","series-title":"Acta Trop","first-page":"233","article-title":"Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test?","volume":"122","author":"Abdul-Ghani\n              R","year":"2012","unstructured":"Abdul-Ghani\n              R\n            , Al-MekhlafiAM, KaranisP. Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test?Acta Trop. 2012;122:233\u201340.22366670"},{"key":"ref6","series-title":"Parasitol Res","first-page":"2439","article-title":"Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China","volume":"110","author":"Lu\n              F","year":"2012","unstructured":"Lu\n              F\n            , GaoQ, ZhouH, CaoJ, WangW, LimCS, et al\n          Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China. Parasitol Res. 2012;110:2439\u201344.22200962"},{"key":"ref7","series-title":"Expert Rev Mol Diagn","first-page":"205","article-title":"Loop-mediated isothermal amplification test for the molecular diagnosis of malaria","volume":"13","author":"Han\n              ET","year":"2013","unstructured":"Han\n              ET\n            \n          . Loop-mediated isothermal amplification test for the molecular diagnosis of malaria. Expert Rev Mol Diagn. 2013;13:205\u201318.23477559"},{"key":"ref8","series-title":"PLoS One","first-page":"e54986","article-title":"Real-time loop-mediated isothermal amplification (RealAmp) for the species-specific identification of Plasmodium vivax","volume":"8","author":"Patel\n              JC","year":"2013","unstructured":"Patel\n              JC\n            , OberstallerJ, XayavongM, NarayananJ, DeBarryJD, SrinivasamoorthyG, et al\n          Real-time loop-mediated isothermal amplification (RealAmp) for the species-specific identification of Plasmodium vivax. PLoS One. 2013;8:e54986.23349994"},{"key":"ref9","series-title":"J Infect Dis","first-page":"1167","article-title":"Point of care testing for malaria using LAMP, loop mediated isothermal amplification","volume":"210","author":"Hsiang\n              MS","year":"2014","unstructured":"Hsiang\n              MS\n            , GreenhouseB, RosenthalPJ. Point of care testing for malaria using LAMP, loop mediated isothermal amplification. J Infect Dis. 2014;210:1167\u20139.24795471"},{"key":"ref10","series-title":"Acta Trop","first-page":"52","article-title":"A new visually improved and sensitive loop mediated isothermal amplification (LAMP) for diagnosis of symptomatic falciparum malaria","volume":"134","author":"Mohon\n              AN","year":"2014","unstructured":"Mohon\n              AN\n            , ElahiR, KhanWA, HaqueR, SullivanDJJr, AlamMS. A new visually improved and sensitive loop mediated isothermal amplification (LAMP) for diagnosis of symptomatic falciparum malaria. Acta Trop. 2014;134:52\u20137.24613155"},{"key":"ref11","series-title":"Malar J","first-page":"396","article-title":"Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa","volume":"14","author":"Oriero\n              EC","year":"2015","unstructured":"Oriero\n              EC\n            , OkebeJ, JacobsJ, Van GeertruydenJP, NwakanmaD, D'AlessandroU. Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa. Malar J. 2015;14:396."},{"key":"ref12","series-title":"Clin Microbiol Infect","first-page":"686","article-title":"Validation of an apicoplast genome target for the detection of Plasmodium species using polymerase chain reaction and loop mediated isothermal amplification","volume":"21","author":"Oriero\n              CE","year":"2015","unstructured":"Oriero\n              CE\n            , GeertruydenJPvan, JacobsJ, D'AlessandroU, NwakanmaD. Validation of an apicoplast genome target for the detection of Plasmodium species using polymerase chain reaction and loop mediated isothermal amplification. Clin Microbiol Infect. 2015;21:686.e1-7.25747504"},{"key":"ref13","series-title":"Malar J.","first-page":"454","article-title":"A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification","volume":"13","author":"Port\n              JR","year":"2014","unstructured":"Port\n              JR\n            , NguetseC, AdukpoS, VelavanTP. A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification. Malar J.2014;13:454.25421401"},{"key":"ref14","series-title":"PLoS Negl Trop Dis","first-page":"e0004443","article-title":"Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination","volume":"10","author":"Britton\n              S","year":"2016","unstructured":"Britton\n              S\n            , ChengQ, GriggMJ, PooleCB, PasayC, WilliamT, et al\n          Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination. PLoS Negl Trop Dis. 2016;10:e0004443.26870958"},{"key":"ref15","series-title":"Am J Trop Med Hyg","first-page":"336","article-title":"Loop-Mediated Isothermal Amplification Assay for Identification of Five Human Plasmodium Species in Malaysia","volume":"94","author":"Lau\n              YL","year":"2016","unstructured":"Lau\n              YL\n            , LaiMY, FongMY, JelipJ, MahmudR. Loop-Mediated Isothermal Amplification Assay for Identification of Five Human Plasmodium Species in Malaysia. Am J Trop Med Hyg. 2016;94:336\u20139.26598573"},{"key":"ref16","series-title":"Diagn Microbiol Infect Dis","first-page":"149","article-title":"NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria","volume":"85","author":"Mohon\n              AN","year":"2016","unstructured":"Mohon\n              AN\n            , LeeLD, BayihAG, FolefocA, GueligD, BurtonRA, et al\n          NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria. Diagn Microbiol Infect Dis. 2016;85:149\u201353.27017271"},{"key":"ref17","series-title":"Rev Inst Med Trop Sao Paulo","first-page":"27","article-title":"MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND","volume":"58","author":"Ocker\n              R","year":"2016","unstructured":"Ocker\n              R\n            , PrompunjaiY, ChutipongvivateS, KaranisP. MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND. Rev Inst Med Trop Sao Paulo. 2016;58:27.27074321"},{"key":"ref18","series-title":"PLoS One","first-page":"e0171126","article-title":"Development and clinical performance of high throughput loop-mediated isothermal amplification for detection of malaria","volume":"12","author":"Perera\n              RS","year":"2017","unstructured":"Perera\n              RS\n            , DingXC, TullyF, OliverJ, BrightN, BellD, et al\n          Development and clinical performance of high throughput loop-mediated isothermal amplification for detection of malaria. PLoS One. 2017;12:e0171126.28166235"},{"key":"ref19","series-title":"J Vector Borne Dis","first-page":"54","article-title":"Evaluation of SYBR green I based visual loop-mediated isothermal amplification (LAMP) assay for genus and species-specific diagnosis of malaria in P. vivax and P. falciparum endemic regions","volume":"54","author":"Singh\n              R","year":"2017","unstructured":"Singh\n              R\n            , SinghDP, SavargaonkarD, SinghOP, BhattRM, ValechaN. Evaluation of SYBR green I based visual loop-mediated isothermal amplification (LAMP) assay for genus and species-specific diagnosis of malaria in P. vivax and P. falciparum endemic regions. J Vector Borne Dis. 2017;54:54\u201360."},{"key":"ref20","series-title":"Pathog Glob Health","first-page":"247","article-title":"Development of loop-mediated isothermal amplification with Plasmodium falciparum unique genes for molecular diagnosis of human malaria","volume":"111","author":"Zhang\n              Y","year":"2017","unstructured":"Zhang\n              Y\n            , YaoY, DuW, WuK, XuW, LinM, et al\n          Development of loop-mediated isothermal amplification with Plasmodium falciparum unique genes for molecular diagnosis of human malaria. Pathog Glob Health. 2017;111:247\u201355.28683669"},{"key":"ref21","series-title":"J Antimicrob Chemother","first-page":"2","article-title":"Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination","volume":"70","author":"Oriero\n              EC","year":"2015","unstructured":"Oriero\n              EC\n            , JacobsJ, Van GeertruydenJP, NwakanmaD, D'AlessandroU. Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination. J Antimicrob Chemother. 2015;70:2\u201313.25223973"},{"key":"ref22","series-title":"Crit Rev Clin Lab Sci","first-page":"87","article-title":"Molecular malaria diagnostics: A systematic review and meta-analysis","volume":"53","author":"Roth\n              JM","year":"2016","unstructured":"Roth\n              JM\n            , KorevaarDA, LeeflangMM, MensPF. Molecular malaria diagnostics: A systematic review and meta-analysis. Crit Rev Clin Lab Sci. 2016;53:87\u2013105.26376713"},{"key":"ref23","series-title":"Can J Infect Dis Med Microbiol","first-page":"9286392","article-title":"Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests","volume":"2017","author":"Mukry\n              SN","year":"2017","unstructured":"Mukry\n              SN\n            , SaudM, SufaidaG, ShaikhK, NazA, ShamsiTS. Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests. Can J Infect Dis Med Microbiol. 2017;2017:9286392.28479922"},{"key":"ref24","series-title":"Sci Rep","first-page":"36808","article-title":"Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites","volume":"6","author":"Lucchi\n              NW","year":"2016","unstructured":"Lucchi\n              NW\n            , GayeM, DialloMA, GoldmanIF, LjoljeD, DemeAB, et al\n          Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites. Sci Rep. 2016;6:36808.27827432"},{"key":"ref25","series-title":"Malar J","first-page":"418","article-title":"Diagnostic performance of the loop-mediated isothermal amplification (LAMP) based illumigene(\u00ae) malaria assay in a non-endemic region","volume":"16","author":"De Koninck\n              AS","year":"2017","unstructured":"De Koninck\n              AS\n            , CnopsL, HofmansM, JacobsJ, Van den BosscheD, Philipp\u00e9J. Diagnostic performance of the loop-mediated isothermal amplification (LAMP) based illumigene(\u00ae) malaria assay in a non-endemic region. Malar J. 2017;16:418."},{"key":"ref26","series-title":"J Clin Microbiol","first-page":"3037","article-title":"Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real-Time PCR","volume":"55","author":"Rypien\n              C","year":"2017","unstructured":"Rypien\n              C\n            , ChowB, ChanWW, ChurchDL, PillaiDR. Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real-Time PCR. J Clin Microbiol. 2017;55:3037\u201345.28768730"},{"key":"ref27","series-title":"Parasite","first-page":"53","article-title":"Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting","volume":"24","author":"Ponce\n              C","year":"2017","unstructured":"Ponce\n              C\n            , KaczorowskiF, PerpointT, MiailhesP, SigalA, JavouheyE, et al\n          Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting. Parasite. 2017;24:53."},{"key":"ref28","series-title":"Travel Med Infect Dis","first-page":"25","article-title":"Evaluation of automated loop-mediated amplification (LAMP) for routine malaria detection in blood samples of German travelers\u2014A cross-sectional study","volume":"24","author":"Frickmann\n              H","year":"2018","unstructured":"Frickmann\n              H\n            , HinzR, RojakS, BonowI, RubenS, WegnerC, et al\n          Evaluation of automated loop-mediated amplification (LAMP) for routine malaria detection in blood samples of German travelers\u2014A cross-sectional study. Travel Med Infect Dis. 2018;24:25\u201330.29763668"},{"key":"ref29","unstructured":"No authors listed. Prise en charge et pr\u00e9vention du paludisme d\u2019importation. Mise \u00e0 jour 2017 des RPC 2007. Available from: http:\/\/www.infectiologie.com\/UserFiles\/File\/spilf\/recos\/2017-palu-texte-final-flash.pdf (last accessed 31st July 2018)."},{"key":"ref30","series-title":"J Clin Microbiol","first-page":"2435","article-title":"Real-time PCR for detection and identification of Plasmodium spp","volume":"43","author":"Mangold\n              KA","year":"2005","unstructured":"Mangold\n              KA\n            , MansonRU, KoayES, StephensL, RegnerM, ThomsonRBJr, et al\n          Real-time PCR for detection and identification of Plasmodium spp. J Clin Microbiol. 2005; 43: 2435\u201340.15872277"},{"key":"ref31","series-title":"Eur J Microbiol Immunol (Bp)","first-page":"159","article-title":"Comparison of two real-time PCR assays for the detection of malaria parasites from hemolytic blood samples - Short communication","volume":"5","author":"Hagen\n              RM","year":"2015","unstructured":"Hagen\n              RM\n            , HinzR, TannichE, FrickmannH. Comparison of two real-time PCR assays for the detection of malaria parasites from hemolytic blood samples - Short communication. Eur J Microbiol Immunol (Bp). 2015;5:159\u201363."},{"key":"ref32","series-title":"Travel Med Infect Dis","first-page":"167","article-title":"Diagnostic accuracy of a LAMP kit for diagnosis of imported malaria in Switzerland","volume":"13","author":"Marti\n              H","year":"2015","unstructured":"Marti\n              H\n            , StalderC, Gonz\u00e1lezIJ. Diagnostic accuracy of a LAMP kit for diagnosis of imported malaria in Switzerland. Travel Med Infect Dis. 2015;13:167\u201371.25623390"},{"key":"ref33","series-title":"Travel Med Infect Dis","first-page":"126","article-title":"LAMP in the context of travellers' malaria: a shining light?","volume":"13","author":"Chiodini\n              PL","year":"2015","unstructured":"Chiodini\n              PL\n            \n          . LAMP in the context of travellers' malaria: a shining light?Travel Med Infect Dis. 2015;13:126\u20137.25770358"},{"key":"ref34","series-title":"Parasitol Int","first-page":"777","article-title":"Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax","volume":"63","author":"Yongkiettrakul\n              S","year":"2014","unstructured":"Yongkiettrakul\n              S\n            , JaroenramW, ArunrutN, ChareanchimW, PannengpetchS, SuebsingR, et al\n          Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax. Parasitol Int. 2014;63:777\u201384.25038579"},{"key":"ref35","series-title":"Methods Mol Biol","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/978-1-4939-6911-1_28","article-title":"Loop-Mediated Isothermal Amplification and LFD Combination for Detection of Plasmodium falciparum and Plasmodium vivax","volume":"1572","author":"Kongkasuriyachai\n              D","year":"2017","unstructured":"Kongkasuriyachai\n              D\n            , YongkiettrakulS, KiatpathomchaiW, ArunrutN. Loop-Mediated Isothermal Amplification and LFD Combination for Detection of Plasmodium falciparum and Plasmodium vivax. Methods Mol Biol. 2017;1572:431\u201343.28299704"},{"key":"ref36","series-title":"Am J Trop Med Hyg","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.18-0177","article-title":"Development of Loop-Mediated Isothermal Amplification-Based Lateral Flow Device Method for the Detection of Malaria","author":"Mallepaddi\n              PC","year":"2018","unstructured":"Mallepaddi\n              PC\n            , LaiMY, PodhaS, OoiCH, LiewJW, PolavarapuR, et al\n          Development of Loop-Mediated Isothermal Amplification-Based Lateral Flow Device Method for the Detection of Malaria. Am J Trop Med Hyg. 2018. doi: 10.4269\/ajtmh.18-0177.29914490"},{"key":"ref37","series-title":"Malar J","first-page":"235","article-title":"In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears","volume":"17","author":"Hashimoto\n              M","year":"2018","unstructured":"Hashimoto\n              M\n            , SakamotoH, IdoY, TanakaM, YatsushiroS, KajimotoK, et al\n          \n            In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears. Malar J. 2018;17:235.25105591"},{"key":"ref38","series-title":"PLoS One","first-page":"e103905","article-title":"Loop mediated isothermal amplification (LAMP) accurately detects malaria DNA from filter paper blood samples of low density parasitaemias","volume":"9","author":"Aydin-Schmidt\n              B","year":"2014","unstructured":"Aydin-Schmidt\n              B\n            , XuW, Gonz\u00e1lezIJ, PolleySD, BellD, ShakelyD, et al\n          Loop mediated isothermal amplification (LAMP) accurately detects malaria DNA from filter paper blood samples of low density parasitaemias. PLoS One. 2014;9:e103905.19168954"},{"key":"ref39","series-title":"Jpn J Infect Dis","first-page":"20","article-title":"Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis","volume":"62","author":"Yamamura\n              M","year":"2009","unstructured":"Yamamura\n              M\n            , MakimuraK, OtaY. Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis. Jpn J Infect Dis. 2009;62:20\u20135.23623995"},{"key":"ref40","series-title":"J Infect","first-page":"245","article-title":"Rapid detection of Plasmodium vivax in saliva and blood using loop mediated isothermal amplification (LAMP) assay","volume":"67","author":"Singh\n              R","year":"2013","unstructured":"Singh\n              R\n            , SavargaonkarD, BhattR, ValechaN. Rapid detection of Plasmodium vivax in saliva and blood using loop mediated isothermal amplification (LAMP) assay. J Infect. 2013;67:245\u20137.24721227"},{"key":"ref41","series-title":"Acta Trop","first-page":"44","article-title":"Detection of Plasmodium vivax and Plasmodium falciparum DNA in human saliva and urine: loop-mediated isothermal amplification for malaria diagnosis","volume":"136","author":"Ghayour Najafabadi\n              Z","year":"2014","unstructured":"Ghayour Najafabadi\n              Z\n            , OormazdiH, AkhlaghiL, MeamarAR, NateghpourM, FarivarL, et al\n          Detection of Plasmodium vivax and Plasmodium falciparum DNA in human saliva and urine: loop-mediated isothermal amplification for malaria diagnosis. Acta Trop. 2014;136:44\u20139."},{"key":"ref42","series-title":"Eur J Microbiol Immunol (Bp)","first-page":"94","article-title":"Comparison of an automated nucleic acid extraction system with the column-based procedure","volume":"5","author":"Frickmann\n              H","year":"2015","unstructured":"Frickmann\n              H\n            , HinzR, HagenRM. Comparison of an automated nucleic acid extraction system with the column-based procedure. Eur J Microbiol Immunol (Bp). 2015;5:94\u2013102."},{"key":"ref43","series-title":"Centralblatt f\u00fcr Bakteriologie","first-page":"307","article-title":"Eine Vereinfachung und Vervollkommnung meiner Methylenblau-Eosin-F\u00e4rbemethode zur Erzielung der Romanowsky-Nocht\u2019schen Chromatinf\u00e4rbung","volume":"32","author":"Giemsa\n              G","year":"1904","unstructured":"Giemsa\n              G\n            \n          . Eine Vereinfachung und Vervollkommnung meiner Methylenblau-Eosin-F\u00e4rbemethode zur Erzielung der Romanowsky-Nocht\u2019schen Chromatinf\u00e4rbung. Centralblatt f\u00fcr Bakteriologie. 1904;32:307\u201313."},{"key":"ref44","author":"Hecht\n              LS","unstructured":"Hecht\n              LS\n            , WegnerC, BunteN, TannichE, HessM. A real-time PCR based Malaria-typing assay for the detection and differentiation of all five human pathogenic Plasmodium species. Poster P80. 12. Kongress f\u00fcr Infektionskrankheiten und Tropenmedizin. 2014; Cologne, Germany."},{"key":"ref45","series-title":"J Clin Microbiol","first-page":"2521","article-title":"Detection of 4 Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis","volume":"45","author":"Han\n              ET","year":"2007","unstructured":"Han\n              ET\n            , WatanabeR, SattabongkotJ, KhuntiratB, SirichaisinthopJ, IrikoH, et al\n          Detection of 4 Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol. 2007;45:2521\u20138.20554824"},{"key":"ref46","series-title":"J Clin Microbiol","first-page":"2866","article-title":"Mitochondrial DNA targets increase sensitivity of malaria detection using loop-mediated isothermal amplification","volume":"48","author":"Polley\n              SD","year":"2010","unstructured":"Polley\n              SD\n            , MoriY, WatsonJ, PerkinsMD, Gonz\u00e1lezIJ, NotomiT, ChiodiniPL, SutherlandCJ. Mitochondrial DNA targets increase sensitivity of malaria detection using loop-mediated isothermal amplification. J Clin Microbiol. 2010;48:2866\u201371."},{"key":"ref47","series-title":"Infect Dis (Auckl)","first-page":"1","article-title":"Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification","volume":"9","author":"Modak\n              SS","year":"2016","unstructured":"Modak\n              SS\n            , BarberCA, GevaE, AbramsWR, MalamudD, OngagnaYS. Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification. Infect Dis (Auckl). 2016;9:1\u20139.10492790"},{"key":"ref48","series-title":"Trans R Soc Trop Med Hyg","first-page":"50","article-title":"Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction","volume":"93","author":"F\u00e4rnert\n              A","year":"1999","unstructured":"F\u00e4rnert\n              A\n            , ArezAP, CorreiaAT, Bj\u00f6rkmanA, SnounouG, do Ros\u00e1rioV. Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction. Trans R Soc Trop Med Hyg. 1999;93:50\u20133.28903726"},{"key":"ref49","series-title":"BMC Infect Dis","first-page":"621","article-title":"A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION\u2122 nanopore sequencer","volume":"17","author":"Imai\n              K","year":"2017","unstructured":"Imai\n              K\n            , TarumotoN, MisawaK, RuntuweneLR, SakaiJ, HayashidaK, et al\n          A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION\u2122 nanopore sequencer. BMC Infect Dis. 2017;17:621.20444968"},{"key":"ref50","series-title":"J Clin Microbiol","first-page":"2509","article-title":"Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi","volume":"48","author":"Iseki\n              H","year":"2010","unstructured":"Iseki\n              H\n            , KawaiS, TakahashiN, HiraiM, TanabeK, YokoyamaN, et al\n          Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi. J Clin Microbiol. 2010;48:2509\u201314.21774805"},{"key":"ref51","series-title":"Malar J","first-page":"197","article-title":"Specific, sensitive and rapid detection of human Plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples","volume":"10","author":"Lau\n              YL","year":"2011","unstructured":"Lau\n              YL\n            , FongMY, MahmudR, ChangPY, PalaeyaV, CheongFW, et al\n          Specific, sensitive and rapid detection of human Plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples. Malar J. 2011;10:197.27162264"},{"key":"ref52","series-title":"Am J Trop Med Hyg","first-page":"120","article-title":"A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi","volume":"95","author":"Britton\n              S","year":"2016","unstructured":"Britton\n              S\n            , ChengQ, GriggMJ, WilliamT, AnsteyNM, McCarthyJS. A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi. Am J Trop Med Hyg. 2016;95:120\u20132."},{"key":"ref53","series-title":"Malar J","first-page":"29","article-title":"Detection of Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax using loop-mediated isothermal amplification (LAMP) in a co-endemic area in Malaysia","volume":"16","author":"Piera\n              KA","year":"2017","unstructured":"Piera\n              KA\n            , AzizA, WilliamT, BellD, Gonz\u00e1lezIJ, BarberBE, et al\n          Detection of Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax using loop-mediated isothermal amplification (LAMP) in a co-endemic area in Malaysia. Malar J. 2017;16:29."},{"key":"ref54","series-title":"J Infect Dis","first-page":"1180","article-title":"Field evaluation of a real-time fluorescence loop-mediated isothermal amplification assay, RealAmp, for the diagnosis of malaria in Thailand and India","volume":"210","author":"Patel\n              JC","year":"2014","unstructured":"Patel\n              JC\n            , LucchiNW, SrivastavaP, LinJT, Sug-AramR, AruncharusS, et al\n          Field evaluation of a real-time fluorescence loop-mediated isothermal amplification assay, RealAmp, for the diagnosis of malaria in Thailand and India. J Infect Dis. 2014;210:1180\u20137.25512623"},{"key":"ref55","series-title":"J Infect Dis","first-page":"1686","article-title":"RealAmp Loop-Mediated Isothermal Amplification as a Point-of-Care Test for Diagnosis of Malaria: Neither Too Close nor Too Far","volume":"211","author":"Goyal\n              K","year":"2015","unstructured":"Goyal\n              K\n            , KaurH, SehgalA, SehgalR. RealAmp Loop-Mediated Isothermal Amplification as a Point-of-Care Test for Diagnosis of Malaria: Neither Too Close nor Too Far. J Infect Dis. 2015;211:1686."},{"key":"ref56","series-title":"Conf Proc IEEE Eng Med Biol Soc","first-page":"1097","article-title":"Non-instrumented nucleic acid amplification (NINA): instrument-free molecular malaria diagnostics for low-resource settings","volume":"2010","author":"Labarre\n              P","year":"2010","unstructured":"Labarre\n              P\n            , GerlachJ, WilmothJ, BeddoeA, SingletonJ, WeiglB. Non-instrumented nucleic acid amplification (NINA): instrument-free molecular malaria diagnostics for low-resource settings. Conf Proc IEEE Eng Med Biol Soc. 2010;2010:1097\u20139."},{"key":"ref57","series-title":"Am J Trop Med Hyg","first-page":"594","article-title":"Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting","volume":"85","author":"Sirichaisinthop\n              J","year":"2011","unstructured":"Sirichaisinthop\n              J\n            , BuatesS, WatanabeR, HanET, SuktawonjaroenponW, KrasaesubS, et al\n          Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting. Am J Trop Med Hyg. 2011;85:594\u20136.21693031"},{"key":"ref58","series-title":"Parasit Vectors","first-page":"115","article-title":"Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection","volume":"4","author":"Tao\n              ZY","year":"2011","unstructured":"Tao\n              ZY\n            , ZhouHY, XiaH, XuS, ZhuHW, CulletonRL, et al\n          Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection. Parasit Vectors. 2011;4:115.26315027"},{"key":"ref59","series-title":"Malar J","first-page":"335","article-title":"A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination","volume":"14","author":"Britton\n              S","year":"2015","unstructured":"Britton\n              S\n            , ChengQ, SutherlandCJ, McCarthyJS. A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination. Malar J. 2015;14:335."},{"key":"ref60","series-title":"PLoS Negl Trop Dis","first-page":"e3453","article-title":"Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia","volume":"9","author":"Vallejo\n              AF","year":"2015","unstructured":"Vallejo\n              AF\n            , Mart\u00ednezNL, Gonz\u00e1lezIJ, Ar\u00e9valo-HerreraM, HerreraS. Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia. PLoS Negl Trop Dis. 2015;9:e3453.27824866"},{"key":"ref61","series-title":"PLoS One","first-page":"e0165506","article-title":"A Field-Tailored Reverse Transcription Loop-Mediated Isothermal Assay for High Sensitivity Detection of Plasmodium falciparum Infections","volume":"11","author":"Kemleu\n              S","year":"2016","unstructured":"Kemleu\n              S\n            , GueligD, Eboumbou MoukokoC, EssanguiE, DiesburgS, MouliomA, et al\n          A Field-Tailored Reverse Transcription Loop-Mediated Isothermal Assay for High Sensitivity Detection of Plasmodium falciparum Infections. PLoS One. 2016;11:e0165506.28982155"},{"key":"ref62","series-title":"PLoS One","first-page":"e0185742","article-title":"Loop-mediated isothermal DNA amplification for asymptomatic malaria detection in challenging field settings: Technical performance and pilot implementation in the Peruvian Amazon","volume":"12","author":"Serra-Casas\n              E","year":"2017","unstructured":"Serra-Casas\n              E\n            , ManriqueP, DingXC, Carrasco-EscobarG, AlavaF, GaveA, et al\n          Loop-mediated isothermal DNA amplification for asymptomatic malaria detection in challenging field settings: Technical performance and pilot implementation in the Peruvian Amazon. PLoS One. 2017;12:e0185742.623091"},{"key":"ref63","series-title":"Am J Epidemiol","first-page":"71","article-title":"Estimating prevalence from the results of a screening test","volume":"107","author":"Rogan\n              WJ","year":"1978","unstructured":"Rogan\n              WJ\n            , GladenB. Estimating prevalence from the results of a screening test. Am J Epidemiol. 1978;107:71\u20136.29353522"},{"key":"ref64","series-title":"Expert Rev Mol Diagn","first-page":"195","article-title":"Expanding the malaria molecular diagnostic options: opportunities and challenges for loop-mediated isothermal amplification tests for malaria control and elimination","volume":"18","author":"Lucchi\n              NW","year":"2018","unstructured":"Lucchi\n              NW\n            , NdiayeD, BrittonS, UdhayakumarV. Expanding the malaria molecular diagnostic options: opportunities and challenges for loop-mediated isothermal amplification tests for malaria control and elimination. Expert Rev Mol Diagn. 2018;18:195\u2013203.29986717"},{"key":"ref65","series-title":"Malar J","first-page":"255","article-title":"Evaluation of loop-mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination","volume":"17","author":"Tambo\n              M","year":"2018","unstructured":"Tambo\n              M\n            , AualaJR, SturrockHJ, KleinschmidtI, BockR, SmithJL, et al\n          Evaluation of loop-mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination. Malar J. 2018;17:255.16339303"}],"container-title":["European Journal of Microbiology and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.akademiai.com\/doi\/full-xml\/10.1556\/1886.2018.00020","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/8\/4\/article-p112.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/8\/4\/article-p112.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/akjournals.com\/downloadpdf\/journals\/1886\/8\/4\/article-p112.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T16:37:26Z","timestamp":1704299846000},"score":1,"resource":{"primary":{"URL":"https:\/\/akjournals.com\/view\/journals\/1886\/8\/4\/article-p112.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":65,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1556\/1886.2018.00020","relation":{},"ISSN":["2062-8633"],"issn-type":[{"value":"2062-8633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12]]}}}